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Development of effective plant-symbiotic microbes system by metagenome and cultivation of non-culturable microbes

Research Project

Project/Area Number 16H06196
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Forest science
Research InstitutionTottori University

Principal Investigator

Taniguchi Takeshi  鳥取大学, 乾燥地研究センター, 准教授 (10524275)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2019: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2016: ¥9,360,000 (Direct Cost: ¥7,200,000、Indirect Cost: ¥2,160,000)
Keywordsメタゲノム / 根圏微生物 / 乾燥地 / 内生菌 / 内生細菌 / 菌根菌 / メタゲノム解析 / 乾燥ストレス / 難培養微生物 / 内部根圏微生物 / トランスクリプトーム解析 / metagenome / metatranscriptome / non-culturable microbes / drought / network analysis / ショットガンメタゲノム / メタトランスクリプトーム解析 / アーバスキュラー菌根菌 / 乾燥 / 根圏 / 複合微生物共生系
Outline of Final Research Achievements

Ecology, functional features, and network analysis of rhizosphere microorganisms were examined to develop effective plant-symbiotic microbes system by using metagenome analysis. As the result, central microbes in the microbial network, Actinomycetes and arbuscular mycorrhizal fungi widely distributed in the research site located in the Colorado desert, California, USA. As endophytic fungi, Ascomycota was dominant and common there compared to Basidiomycota. Under the drought, root endophytic fungal and bacterial communities differed and the changes in rhizosphere microbial function under the drought were indicated by enzymatic activity and functional gene abundances. For establishment of effective plant-symbiotic microbes system, effective microbes were estimated by adaptability to environments and microbial network analysis, but the actual function should be examined in the future.

Academic Significance and Societal Importance of the Research Achievements

本研究は、利用可能な水分や養分が限られた厳しいストレス環境である乾燥地を対象として行われた。地球温暖化による降雨パターンの変動や乾燥化が多くの地域で予想されているため、本研究で得られた乾燥地における微生物の生態や機能に関する知見は、生態系や微生物機能の乾燥応答を予想する上で役立つものである。また、効果的な微生物の分離培養法や分離培養が難しい根圏微生物に関する情報は、利用できる微生物を増やすことに貢献する可能性があり、乾燥地の環境修復や農業への微生物利用を大きく発展させる上でも有用な研究成果である。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (41 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (4 results) Journal Article (16 results) (of which Int'l Joint Research: 13 results,  Peer Reviewed: 15 results,  Open Access: 3 results) Presentation (17 results) (of which Int'l Joint Research: 1 results) Book (1 results) Remarks (3 results)

  • [Int'l Joint Research] カリフォルニア大学リバーサイド校(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] カリフォルニア大学リバーサイド校(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] カリフォルニア大学リバーサイド校/Deep Canyon Desert Research Institute(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] カリフォルニア大学リバーサイド校(米国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Communities of arbuscular mycorrhizal fungi in forest ecosystems in Japan’s temperate region may be primarily constituted by limited fungal taxa.2020

    • Author(s)
      Miyake H., Ishitsuka S., Taniguchi T., Yamato M.
    • Journal Title

      Mycorrhiza

      Volume: ― Issue: 2-3 Pages: 257-268

    • DOI

      10.1007/s00572-020-00945-z

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Soil prokaryotic community structure is determined by a plant-induced soil salinity gradient rather than other environmental parameters associated with plant presence in a saline grassland2020

    • Author(s)
      Tatsumi Chikae、Imada Shogo、Taniguchi Takeshi、Du Sheng、Yamanaka Norikazu、Tateno Ryunosuke
    • Journal Title

      Journal of Arid Environments

      Volume: 176 Pages: 104100-104100

    • DOI

      10.1016/j.jaridenv.2020.104100

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Soil nitrogen cycling is determined by the competition between mycorrhiza and ammonia‐oxidizing prokaryotes2020

    • Author(s)
      Tatsumi Chikae、Taniguchi Takeshi、Du Sheng、Yamanaka Norikazu、Tateno Ryunosuke
    • Journal Title

      Ecology

      Volume: 101 Issue: 3 Pages: 1-10

    • DOI

      10.1002/ecy.2963

    • NAID

      120006810862

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effects of livestock grazing intensity on soil arbuscular mycorrhizal fungi and glomalin-related soil protein in a mountain forest steppe and a desert steppe of Mongolia.2020

    • Author(s)
      Goomaral, A., Yamato, M., Kusakabe, R., Undarmaa, J., Yamanaka, N., Taniguchi, T.
    • Journal Title

      Landscape and Ecological Engineering

      Volume: ― Issue: 3 Pages: 253-265

    • DOI

      10.1007/s11355-019-00399-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effects of Land Use and Topographic Position on Soil Organic Carbon and Total Nitrogen Stocks in Different Agro-Ecosystems of the Upper Blue Nile Basin2020

    • Author(s)
      Abebe Getu、Tsunekawa Atsushi、Haregeweyn Nigussie、Takeshi Taniguchi、Wondie Menale、Adgo Enyew、Masunaga Tsugiyuki、Tsubo Mitsuru、Ebabu Kindiye、Berihun Mulatu Liyew、Tassew Asaminew
    • Journal Title

      Sustainability

      Volume: 12 Issue: 6 Pages: 2425-2425

    • DOI

      10.3390/su12062425

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Does conversion from natural forest to plantation affect fungal and bacterial biodiversity, community structure, and co-occurrence networks in the organic horizon and mineral soil?2019

    • Author(s)
      Nakayama Masataka、Imamura Shihomi、Taniguchi Takeshi、Tateno Ryunosuke
    • Journal Title

      Forest Ecology and Management

      Volume: 446 Pages: 238-250

    • DOI

      10.1016/j.foreco.2019.05.042

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Dominance of limited arbuscular mycorrhizal fungal generalists of Sorghum bicolor in a semi-arid region in Sudan2019

    • Author(s)
      Badi Omer Badi Mohammed、Abdelhalim Tilal Sayed、Eltayeb Mohamed Mutasim、Gorafi Yasir Serag Alnor、Tsujimoto Hisashi、Taniguchi Takeshi
    • Journal Title

      Soil Science and Plant Nutrition

      Volume: 65 Issue: 6 Pages: 570-578

    • DOI

      10.1080/00380768.2019.1680573

    • NAID

      210000157471

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] High salt tolerant plant growth promoting rhizobacteria from the common ice-plant Mesembryanthemum crystallinum L2019

    • Author(s)
      Mahmood A., Amaya R., Turgay O.C., Yaprak A.E., Taniguchi T., Kataoka R.
    • Journal Title

      Rhizosphere

      Volume: 9 Pages: 10-17

    • DOI

      10.1016/j.rhisph.2018.10.004

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Arbuscular mycorrhizal fungal communities under gradients of grazing in Mongolian grasslands of different aridity2018

    • Author(s)
      Kusakabe R., Taniguchi T., Goomaral A., Undarmaa J., Yamanaka N., Yamato M.
    • Journal Title

      Mycorrhiza

      Volume: 28 Issue: 7 Pages: 621-634

    • DOI

      10.1007/s00572-018-0855-7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A pulse of summer precipitation after the dry season triggers changes in ectomycorrhizal formation, diversity, and community composition in a Mediterranean forest in California, USA.2018

    • Author(s)
      Taniguchi T., Kitajima K., Douhan G.W., Yamanaka N., Allen M.F.
    • Journal Title

      Mycorrhiza

      Volume: 28 Issue: 7 Pages: 665-677

    • DOI

      10.1007/s00572-018-0859-3

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Net primary production, nitrogen cycling, biomass allocation, and resource use efficiency along a topographical soil water and nitrogen gradient in a semi-arid forest near an arid boundary.2017

    • Author(s)
      Tateno, R., T. Taniguchi, J. Zhang, W. Shi, J. Zhang, S. Du and N. Yamanaka
    • Journal Title

      Plant and Soil

      Volume: 420 Issue: 1-2 Pages: 209-222

    • DOI

      10.1007/s11104-017-3390-y

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 根圏微生物とその生態系修復や農業生産への利用2017

    • Author(s)
      谷口武士
    • Journal Title

      アグリバイオ

      Volume: 1 Pages: 26-30

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Identification of the key genes involved in the degradation of homocholine by Pseudomonas sp. strain A9 by using suppression subtractive hybridization.2017

    • Author(s)
      2)Mohamed M.E, Habora M.E., Eltayeb A.E., Mori N., Arima J., Taniguchi T., Yamanaka N.
    • Journal Title

      Process Biochemistry

      Volume: 52 Pages: 94-105

    • DOI

      10.1016/j.procbio.2016.10.009

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Phytoremediation of calcareous saline-sodic soils with mesquite (Prosopis glandulosa).2017

    • Author(s)
      3)Abdalla M.A., Taniguchi T., Elkarim A.H.A., Endo T., Yamanaka N.
    • Journal Title

      Acta Agriculturae Scandinavica, Section B - Plant Soil Science

      Volume: 67 Issue: 4 Pages: 352-361

    • DOI

      10.1080/09064710.2017.1281432

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Land-use types and soil chemical properties influence soil microbial communities in the semiarid Loess Plateau region in China2017

    • Author(s)
      Qin Tian, Takeshi Taniguchi, Wei-Yu Shi, Guoqing Li, Norikazu Yamanaka, and Sheng D.
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 45289-45289

    • DOI

      10.1038/srep45289

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Inorganic and Organic Osmolytes Accumulation in Five Halophytes Growing in Saline Habitats around Aiding Lake Area in Turpan Basin, Northwest China2016

    • Author(s)
      Maimaiti, A., F. Iwanaga, T. Taniguchi, N. Hara, N. Matsuo, N. Mori, Q. Yunus, N. Yamanaka
    • Journal Title

      Arid Land Research and Management

      Volume: 30 Issue: 4 Pages: 421-431

    • DOI

      10.1080/15324982.2016.1148799

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 北海道東部の森林における土壌微生物群集と窒素無機化ポテンシャルの関係2020

    • Author(s)
      中山理智, 今村志帆美, 龍見史恵, 谷口武士, 舘野隆之輔
    • Organizer
      第131回日本森林学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Microbial function of a desert plant, Encelia farinosa rhizosphere under drought2020

    • Author(s)
      Eltayeb, M.M., Eltigani, S., Isobe, K., Aronson, E., Taniguchi, T.
    • Organizer
      第67回日本生態学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 家畜による被食が土と根のアーバスキュラー菌根群集に与える影響2020

    • Author(s)
      谷口武士, 大和政秀, 日下部亮太, 赤路康朗, Goomaral Altansukh, Undarmaa Jamsran, 山中典和
    • Organizer
      第67回日本生態学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ヒルギ科二種の根内および根近傍土壌に存在するアーバスキュラー菌根菌群集構造2020

    • Author(s)
      赤路康朗, 井上智美, 谷口武士, 馬場繁幸
    • Organizer
      第67回日本生態学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 乾燥地における植物内部根圏キーストーン微生物の宿主特異性2019

    • Author(s)
      谷口 武士, 磯部 一夫, 今田 省吾, Mohammed M Eltayeb, 赤路 康朗, Michael F Allen, Emma Aronson
    • Organizer
      第66回日本生態学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] コロラド砂漠で生育する乾燥地植物6種の内部根圏微生物とその宿主特異性2018

    • Author(s)
      谷口武士,Mohammed Mutasim Eltayeb Elebeid, 今田省吾,磯部一夫, Michael Allen, Rebecca Hermamdez, Emma Aronson
    • Organizer
      第129回日本森林学会大会プログラム
    • Related Report
      2017 Annual Research Report
  • [Presentation] Effect of drought, microbes and Encelia farinosa on soil extracellular enzymes2018

    • Author(s)
      Mohamed M. Eltayeb, Emma Aronson, Sara Eltigani, Kazuo Isobe, Norikazu Yamanaka, Jiro Arima, Michael Allen, Takeshi Taniguchi
    • Organizer
      第129回日本森林学会大会プログラム
    • Related Report
      2017 Annual Research Report
  • [Presentation] Genotypic variation in mycorrhizal community structure and host specificity of Sudanese sorghums2017

    • Author(s)
      Omer Badi Mohammed Badi, Tilal Sayed Abdelhalim, Yasir S. A. Mohammed, Hisashi Tsujimoto, Takeshi Taniguchi
    • Organizer
      第243回日本作物学会講演会
    • Place of Presentation
      東京大学農学部弥生キャンパス(東京都文京区)
    • Year and Date
      2017-03-29
    • Related Report
      2016 Annual Research Report
  • [Presentation] 植物ホルモン処理がニセアカシアの萌芽発生に及ぼす影響2017

    • Author(s)
      片山卓弥、笹原育美、沖田総一郎、岩永史子、谷口武士、山本福壽、山中典和
    • Organizer
      第128回日本森林学会大会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] 酸欠ストレスがマングローブ樹種の耐塩性に及ぼす影響2017

    • Author(s)
      沖田総一郎、谷口真吾、谷口武士、山本福壽、山中典和
    • Organizer
      第128回日本森林学会大会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] 異なるNaCl処理が根粒菌を接種したマメ科樹種の生存・生育に与える影響2017

    • Author(s)
      笹原育美、谷口武士、山中典和
    • Organizer
      第128回日本森林学会大会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] 津波被害後の海岸クロマツ林における菌根相2017

    • Author(s)
      竹内祐子、谷口武士、片岡良太、平本将久、中村慎崇
    • Organizer
      第128回日本森林学会大会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-26
    • Related Report
      2016 Annual Research Report
  • [Presentation] ブナ実生の資源分配と菌根共生に微環境が与える影響2017

    • Author(s)
      赤路康朗、山田和弘、廣部宗、谷口武士、宮崎祐子、坂本圭児
    • Organizer
      第64回日本生態学会大会
    • Place of Presentation
      早稲田大学早稲田キャンパス(東京都新宿区)
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] DNAバーコーディングを用いたモンゴル乾燥地域における野生草食動物と家畜の食性重複の解明2017

    • Author(s)
      杉本太郎、伊藤健彦、谷口武士、Lhagvasuren Badamjav、Tsendsuren Oyunsuren、坂本有実、山中典和
    • Organizer
      第64回日本生態学会大会
    • Place of Presentation
      早稲田大学早稲田キャンパス(東京都新宿区)
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 半乾燥地の森林土壌における真菌・原核生物機能群組成と窒素動態2017

    • Author(s)
      岩岡史恵、谷口武士、山中典和、杜盛、舘野隆之輔
    • Organizer
      第64回日本生態学会大会
    • Place of Presentation
      早稲田大学早稲田キャンパス(東京都新宿区)
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 降水量と家畜食害強度の違いがモンゴル草原のアーバスキュラー菌根菌群集に与える影響2017

    • Author(s)
      日下部亮太、谷口武士、Undarmaa Jamssran、山中典和、Goomaral Altansukh、大和政秀
    • Organizer
      第64回日本生態学会大会
    • Place of Presentation
      早稲田大学早稲田キャンパス(東京都新宿区)
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] Revegetation of a degraded ecosystem in dry environments: Experiences from China.2016

    • Author(s)
      Takeshi Taniguchi, Norikazu Yamanaka
    • Organizer
      The twelfth international conference on development of drylands
    • Place of Presentation
      Bibliotheca Alexandrina (Alexandria, Egypt)
    • Year and Date
      2016-08-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Book] 持続可能な乾燥地農業のために : 土壌塩類化防止と塩類土壌修復2019

    • Author(s)
      谷口武士・片岡良太
    • Total Pages
      202
    • Publisher
      技報堂出版
    • ISBN
      9784765534734
    • Related Report
      2018 Annual Research Report
  • [Remarks] google scholar

    • URL

      https://scholar.google.co.jp/citations?user=UMKPkHUAAAAJ&hl=ja

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
  • [Remarks] 研究代表者の紹介

    • URL

      http://www.alrc.tottori-u.ac.jp/staff303/biseibutu/lu_li.html

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
  • [Remarks]

    • URL

      http://www.alrc.tottori-u.ac.jp/staff303/biseibutu/lu_li.html

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2022-01-28  

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