| Project/Area Number |
16H06197
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| Research Category |
Grant-in-Aid for Young Scientists (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Forest science
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| Research Institution | Forest Research and Management Organization |
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Principal Investigator |
Worth James 国立研究開発法人森林研究・整備機構, 森林総合研究所, 主任研究員 等 (30770771)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥21,710,000 (Direct Cost: ¥16,700,000、Indirect Cost: ¥5,010,000)
Fiscal Year 2018: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2017: ¥8,840,000 (Direct Cost: ¥6,800,000、Indirect Cost: ¥2,040,000)
Fiscal Year 2016: ¥7,150,000 (Direct Cost: ¥5,500,000、Indirect Cost: ¥1,650,000)
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| Keywords | forest / climate change / genetic diversity / hybridisation / Tsuga diversifolia / Tsuga sieboldii / Fagus crenata / NGS / SSR markers / population genetics / Japanese Tsuga / Range-edge populations / Adaptive genes / adaptation / resilience / cool temperate forests / Climate change / Resilience / Adaptation / Cool temperate forests / Genetic diversity |
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| Outline of Final Research Achievements |
Overall the study designed the first nuclear and chloroplast genetic markers for Japanese Tsuga species, the temperate T. sieboldii and subalpine T. diversifolia. Range-wide population genetic studies of both species found significant genetic structure with the most diverged populations being the southern and northern most occurrences in both species. Genetic markers also were used to discover the southernmost population of T. diversiolia at Mt Ishizuchi, Ehime Prefecture, which consists of a mixed stand of both species. Although not identified using SSRs, RAD-seq data found evidence for low levels of past hybridisation between the two Tsuga species in southern edge populations.
For Fagus crenata, whole chloroplast genome sequencing revealed two strongly diverged clades, one in western Japan that is closest to Chinese Fagus species and the other in eastern Japan with uncertain affinity. RAD-seq data for 354 samples from four elevational clines has been obtained and analysis is ongoing.
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| Academic Significance and Societal Importance of the Research Achievements |
Genetic isolation, hybridisation and competition with the temperate T. sieboldii threaten the long term viability of southern range-edge populations of the major Japanese subalpine tree Tsuga diversifolia.
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