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Development and Applications of Chemical Probes for Visualization of Plant Cell Wall Lignification

Research Project

Project/Area Number 16H06198
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Wood science
Research InstitutionKyoto University

Principal Investigator

Tobimatsu Yuki  京都大学, 生存圏研究所, 准教授 (20734221)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥24,570,000 (Direct Cost: ¥18,900,000、Indirect Cost: ¥5,670,000)
Fiscal Year 2019: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2018: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2016: ¥16,640,000 (Direct Cost: ¥12,800,000、Indirect Cost: ¥3,840,000)
Keywordsリグニン / 植物細胞壁 / 木質バイオマス / 生体イメージング / 蛍光プローブ / クリックケミストリー / バイオマス / 蛍光イメージング / 細胞壁
Outline of Final Research Achievements

In this project, we have developed a new tool for visualizing plant cell wall lignification by using synthetic chemical probes. The developed method can be implemented in various research settings aiming to investigate the elusive mechanisms of plant cell wall development and ultimately facilitate the use of plant cell wall materials as sustainable biomass resource. With collaborators, we explore the utility of the developed chemical probes to visualize the dynamics of plant cell wall lignification processes in various plant tissues and obtained several new insights into the molecular mechanisms associated with the development of plant cell walls.

Academic Significance and Societal Importance of the Research Achievements

本研究では、従来困難であった木質細胞壁形成の簡便かつ高感度なイメージング解析を可能とする新手法を開発し、それを活用して木質形成の分子機構に関わる様々な新知見を得た。これらは、陸上植物の進化や環境適応と密接に関わる植物細胞壁の構造・形成・機能の理解に大きく寄与するとともに、持続型社会構築を担う木質バイオマス利用の促進のための基盤構築に貢献する成果である。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (48 results)

All 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (5 results) Journal Article (20 results) (of which Int'l Joint Research: 14 results,  Peer Reviewed: 18 results,  Open Access: 11 results,  Acknowledgement Compliant: 1 results) Presentation (18 results) (of which Int'l Joint Research: 2 results,  Invited: 11 results) Book (4 results) Remarks (1 results)

  • [Int'l Joint Research] ウィスコンシン大学/オクラホマ大学(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] 高麗大学校(韓国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] ウィスコンシン大学(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] University of Wisconsin-Madison(米国)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] University of Wisconsin-Madison(米国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Double knockout of OsWRKY36 and OsWRKY102 boosts lignification with altering culm morphology of rice.2020

    • Author(s)
      Takuji Miyamoto, Rie Takada, Yuki Tobimatsu, Shiro Suzuki, Masaomi Yamamura, Keishi Osakabe, Yuriko Osakabe, Masahiro Sakamoto, Toshiaki Umezawa
    • Journal Title

      Plant Science

      Volume: in press Pages: 110466-110466

    • DOI

      10.1016/j.plantsci.2020.110466

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Convergent recruitment of 5´‐hydroxylase activities by CYP75B flavonoid B‐ring hydroxylases for tricin biosynthesis in Medicago legumes2020

    • Author(s)
      Andy CW Lui, Pui Ying Lam, Chan Kwun-Ho, Lanxiang Wang, Yuki Tobimatsu, Clive Lo
    • Journal Title

      New Phytologist

      Volume: in press Issue: 1 Pages: 269-284

    • DOI

      10.1111/nph.16498

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Fiber‐specific regulation of lignin biosynthesis improves biomass quality in Populus2020

    • Author(s)
      Jinshan Gui, Pui Ying Lam, Yuki Tobimatsu, Jiayan Sun, Cheng Huang, Shumin Cao, Yu Zhong, Toshiaki Umezawa, Laigeng Li
    • Journal Title

      New Phytologist

      Volume: 226 Issue: 4 Pages: 1074-1087

    • DOI

      10.1111/nph.16411

    • NAID

      120006898061

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Lignin-inspired surface modification of nanocellulose by enzyme-catalyzed radical coupling of coniferyl alcohol in Pickering emulsion2020

    • Author(s)
      Kyohei Kanomata, Naoya Fukuda, Takuma Miyata, Pui Ying Lam, Toshiyuki Takano, Yuki Tobimatsu, Takuya Kitaoka
    • Journal Title

      ACS Sustainable Chemistry & Engineering

      Volume: 8 Issue: 2 Pages: 1185-1194

    • DOI

      10.1021/acssuschemeng.9b06291

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Altered lignocellulose chemical structure and molecular assembly in CINNAMYL ALCOHOL DEHYDROGENASE-deficient rice2019

    • Author(s)
      Martin, A.F., Tobimatsu, Y., Kusumi, R., Matsumoto, N., Miyamoto, T., Lam, P.Y., Yamamura, M., Koshiba, T., Sakamoto, M., Umezawa, T.
    • Journal Title

      Science Reports

      Volume: 9 Issue: 1 Pages: 17153-17153

    • DOI

      10.1038/s41598-019-53156-8

    • NAID

      120006893975

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Lignin‐based barrier restricts pathogens to the infection site and confers resistance in plants2019

    • Author(s)
      Myoung‐Hoon Lee, Hwi Seong Jeon, Seu Ha Kim, Joo Hee Chung, Daniele Roppolo, Hye‐Jung Lee, Hong Joo Cho, Yuki Tobimatsu, John Ralph & Ohkmae K Park
    • Journal Title

      The EMBO Journal

      Volume: - Issue: 23

    • DOI

      10.15252/embj.2019101948

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] OsCAldOMT1 is a bifunctional O-methyltransferase involved in the biosynthesis of tricin-lignins in rice cell walls2019

    • Author(s)
      Pui Ying Lam, Yuki Tobimatsu, Naoyuki Matsumoto, Shiro Suzuki, Wu Lan, Yuri Takeda, Masaomi Yamamura, Masahiro Sakamoto, John Ralph, Clive Lo & Toshiaki Umezawa
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 11597-11597

    • DOI

      10.1038/s41598-019-47957-0

    • NAID

      120006822515

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Recruitment of specific flavonoid B-ring hydroxylases for two independent biosynthesis pathways of flavone-derived metabolites in grasses2019

    • Author(s)
      Pui Ying Lam, Andy Lui, Masaomi Yamamura, Lanxiang Wang, Yuri Takeda, Shiro Suzuki, Hongjia Liu, Fu-Yuan Zhu, Mo-Xian Chen, Jian-Hua Zhang, Toshiaki Umezawa, Yuki Tobimatsu, Clive Lo
    • Journal Title

      New Phytologist

      Volume: 印刷中 Issue: 1 Pages: 2014-2019

    • DOI

      10.1111/nph.15795

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Lignin polymerization: how do plants manage the chemistry so well?2019

    • Author(s)
      Tobimatsu Yuki、Schuetz Mathias
    • Journal Title

      Current Opinion in Biotechnology

      Volume: 56 Pages: 75-81

    • DOI

      10.1016/j.copbio.2018.10.001

    • NAID

      120006534615

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] OsMYB108 loss-of-function enriches p-coumaroylated and tricin lignin units in rice cell walls2019

    • Author(s)
      Takuji Miyamoto, Rie Takada, Yuki Tobimatsu, Yuli Takeda, Shiro Suzuki, Masaomi Yamamura, Keishi Osakabe, Yuriko Osakabe, Masahiro Sakamoto, Toshiaki Umezawa
    • Journal Title

      Plant Journal

      Volume: 印刷中 Issue: 6 Pages: 975-987

    • DOI

      10.1111/tpj.14290

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Structural Integrity of Lignin Is Crucial for Resistance against Striga hermonthica Parasitism in Rice2019

    • Author(s)
      Mutuku J. Musembi、Cui Songkui、Hori Chiaki、Takeda Yuri、Tobimatsu Yuki、Nakabayashi Ryo、Mori Tetsuya、Saito Kazuki、Demura Taku、Umezawa Toshiaki、Yoshida Satoko、Shirasu Ken
    • Journal Title

      Plant Physiology

      Volume: 179 Issue: 4 Pages: 1796-1809

    • DOI

      10.1104/pp.18.01133

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Comparative evaluations of lignocellulose reactivity and usability in transgenic rice plants with altered lignin composition2019

    • Author(s)
      Takeda Yuri、Tobimatsu Yuki、Yamamura Masaomi、Takano Toshiyuki、Sakamoto Masahiro、Umezawa Toshiaki
    • Journal Title

      Journal of Wood Science

      Volume: 65 Issue: 1 Pages: 6-6

    • DOI

      10.1186/s10086-019-1784-6

    • NAID

      120006559417

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lignin characterization of rice CONIFERALDEHYDE 5‐HYDROXYLASE loss‐of‐function mutants generated with the CRISPR/Cas9 system.2019

    • Author(s)
      Yuri Takeda, Shiro Suzuki, Yuki Tobimatsu, Keishi Osakabe, Yuriko Osakabe, Safendrri Komara Ragamustari, Masahiro Sakamoto, Toshiaki Umezawa
    • Journal Title

      Plant Journal

      Volume: 97 Issue: 3 Pages: 543-554

    • DOI

      10.1111/tpj.14141

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] リグニンの構造多様性とバイオマス利用に 向けた代謝工学2019

    • Author(s)
      飛松裕基
    • Journal Title

      月刊バイオインダストリー

      Volume: in press

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Downregulation of p‐COUMAROYL ESTER 3‐HYDROXYLASE in rice leads to altered cell wall structures and improves biomass saccharification2018

    • Author(s)
      Yuri Takeda, Yuki Tobimatsu,Steven D. Karlen, Taichi Koshiba, Shiro Suzuki,Masaomi Yamamura, Shinya Murakami, Mai Mukai, Takefumi Hattori, Keishi Osakabe, John Ralph, Masahiro Sakamoto, Toshiaki Umezawa
    • Journal Title

      Plant Journal

      Volume: 95 Issue: 5 Pages: 796-811

    • DOI

      10.1111/tpj.13988

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] NMR studies on lignocellulose deconstructions in the digestive system of the lower termite Coptotermes formosanus Shiraki2018

    • Author(s)
      Tarmadi D, Tobimatsu Y, Yamamura M, Miyamoto T, Miyagawa Y, Umezawa T, Yoshimura T.
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 1290-1290

    • DOI

      10.1038/s41598-018-19562-0

    • NAID

      120006502583

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Host lignin composition affects haustorium induction in the parasitic plants Phtheirospermum japonicum and Striga hermonthica2018

    • Author(s)
      Cui S, Wada S, Tobimatsu Y, Takeda Y, Saucet S, Takano T, Umezawa T, Shirasu K, Yoshida S
    • Journal Title

      New Phytologist

      Volume: 218 Issue: 2 Pages: 710-723

    • DOI

      10.1111/nph.15033

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Disrupting Flavone Synthase II alters lignin and improves biomass digestibility2017

    • Author(s)
      Lam PY, Tobimatsu Y, Takeda Y, Suzuki S, Yamamura M, Umezawa T, Lo C
    • Journal Title

      Plant Physiology

      Volume: 印刷中 Issue: 2 Pages: 972-985

    • DOI

      10.1104/pp.16.01973

    • NAID

      120007189283

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 植物と人を支える細胞壁の科学2017

    • Author(s)
      飛松裕基
    • Journal Title

      生存圏研究

      Volume: 13 Pages: 10-18

    • NAID

      120006496867

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A ‘‘Double Click’’ for Illuminating Plant Cell Walls2017

    • Author(s)
      Yuki Tobimatsu
    • Journal Title

      Cell Chemical Biology

      Volume: 24 Issue: 3 Pages: 246-247

    • DOI

      10.1016/j.chembiol.2017.03.007

    • NAID

      120007189284

    • Related Report
      2016 Annual Research Report
    • Acknowledgement Compliant
  • [Presentation] Bioimaging of fluorophore-tagged monolignols reveals lignification in the parasitic plant organ haustorium2020

    • Author(s)
      Cui Songkui, Yuri Takeda, Yuki Tobimatsu, Satoko Yoshida
    • Organizer
      第61回日本植物生理学会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ヒノキ圧縮あて材における G および H リグニンの 沈着に関わるラッカーゼ(CoLac1 と CoLac3)2020

    • Author(s)
      平出秀人、飛松裕基、福島和彦、松下泰幸、小林優、吉永新、髙部圭司
    • Organizer
      第70回日本木材学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 樹木の木化に学ぶ Pickering エマルションを用いた ナノセルロースの表面修飾2020

    • Author(s)
      福田直弥、鹿又喬平、宮田拓摩、Pui Ying Lam、高野俊幸、飛松裕基、北岡卓也
    • Organizer
      第70回日本木材学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] イネ科バイオマスを特徴付けるリグニンの構造多様性とその形成制御2020

    • Author(s)
      飛松裕基
    • Organizer
      岡山大学資源植物科学研究所 第3回作物イノベーション研究セミナー・植物機能性成分研究の最前線
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] A laccase oxidizes p-coumaryl alcohol in the compression wood cell walls of Japanese cypress2019

    • Author(s)
      Hideto Hiraide, Yuki Tobimatsu, Yasuyuki Matsushita, Kazuhiko Fukushima, Masaru Kobayashi, Arata Yoshinaga, Keiji Takabe
    • Organizer
      1st International Lignin Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 植物細胞壁の形成と制御 -持続可能なバイオ燃料・有用化成品の生産に向けて-2019

    • Author(s)
      飛松裕基
    • Organizer
      第33回京都大学宇治キャンパス産学交流会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] ヒノキ圧縮あて材分化中木部においてラッカーゼのp-クマリルアルコール酸化重合活性を可視化する2019

    • Author(s)
      平出秀人,飛松裕基,吉永新,髙部圭司
    • Organizer
      第69回日本木材学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 代謝工学で探るセルロース系多糖とリグニンの微妙な関係:リグニン改変組換え植物におけるリグノセルロース特性の変化について2019

    • Author(s)
      飛松裕基
    • Organizer
      セルロース学会北海道・東北支部セミナー:何が問題、セルロース
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] 代謝工学で探る細胞壁超分子構造に及ぼすリグニンの寄与2019

    • Author(s)
      飛松裕基
    • Organizer
      第3回動的木材化学懇談会
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] ヒノキ圧縮あて材細胞壁においてラッカーゼの p-クマリルアルコール取込み能力を可視化する2019

    • Author(s)
      平出秀人、飛松裕基、吉永新、高部圭司
    • Organizer
      第63回リグニン討論会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 蛍光プローブによるポプラ培養細胞の管状要素誘導系におけるリグニン沈着の可視化2018

    • Author(s)
      吉田裕子,山岸祐介,飛松裕基,梅澤俊明,半 智史,船田 良
    • Organizer
      第68回日本木材学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 自然に学ぶ木質の新デザイン:リグニンの多様性と可変性2017

    • Author(s)
      飛松裕基
    • Organizer
      第10回木質科学シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Plant Cell Wall Structure and Chemistry2017

    • Author(s)
      Yuki Tobimatsu
    • Organizer
      Humanosphere Science School 2017
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Secondary Cell Wall Formation and Lignification: Diversity, Flexibility, and Scope for Improvement2017

    • Author(s)
      Yuki Tobimatsu
    • Organizer
      SATREPS Capacity Developmental Seminar
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 組換え植物で探るリグニン化学構造の進化的変化の意味とバイオマス利用へのインパクト2017

    • Author(s)
      飛松裕基
    • Organizer
      第2回動的木材化学懇談会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 細胞壁リグニンの多様性とバイオマス利用に向けた構造改変2017

    • Author(s)
      飛松裕基
    • Organizer
      第1回生物資源・次世代農業セミナー「循環型炭素資源ー植物 細胞壁の研究」
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Plant Cell Wall Lignification: Diversity, Flexibility, and Scope for Improvement2016

    • Author(s)
      Yuki Tobimatsu
    • Organizer
      The Hong Kong University Plant Evolution & Adaptation Workshop 2016 INTEGRATIVE PLANT BIOLOGY
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 植物と人を“支える”細胞壁の科学2016

    • Author(s)
      飛松裕基
    • Organizer
      第13回生存圏研究所公開講演
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Book] リグニン利活用のための最新技術動向2020

    • Author(s)
      飛松裕基
    • Publisher
      シーエムシー出版
    • ISBN
      9784781314945
    • Related Report
      2019 Annual Research Report
  • [Book] 食品・バイオにおける最新の酵素応用2019

    • Author(s)
      飛松裕基
    • Publisher
      シーエムシー出版
    • ISBN
      9784781314266
    • Related Report
      2019 Annual Research Report
  • [Book] 月刊 アグリバイオ 2019年6月号2019

    • Author(s)
      飛松裕基, Pui Ying Lam, 梅澤俊明, Clive Lo
    • Publisher
      北隆館
    • Related Report
      2019 Annual Research Report
  • [Book] Lignin: Biosynthesis, Functions, and Economic Significance2019

    • Author(s)
      Yuki Tobimatsu, Toshiyuki Takano, Toshiaki Umezawa, and John Ralph
    • Publisher
      Nova Science Publishers Inc.
    • ISBN
      9781536147698
    • Related Report
      2019 Annual Research Report
  • [Remarks] マンガ 生存圏って何?? 「植物細胞壁ってナニ」(原作:飛松裕基 作画:田中翔斗)

    • URL

      http://www.rish.kyoto-u.ac.jp/logos/wp-content/uploads/2015/11/Manga_020_No.18_Cell_wall_ja.pdf

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2022-05-23  

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