Characterization of gill epithelial antigen sampling cells

• Project/Area Number: 16H06201
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Single-year Grants
• Research Field: Aquatic bioproduction science
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: Kato Goshi 東京海洋大学, 学術研究院, 助教 (50624219)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥24,440,000 (Direct Cost: ¥18,800,000、Indirect Cost: ¥5,640,000); Fiscal Year 2018: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2017: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2016: ¥12,740,000 (Direct Cost: ¥9,800,000、Indirect Cost: ¥2,940,000)
• Keywords: 粘膜免疫 / 浸漬ワクチン / 魚類免疫学 / 水産学 / 感染症 / 細胞・組織 / 海洋科学 / 魚類免疫 / 免疫学

Outline of Final Research Achievements

Bath-vaccination is a cost-effective technique to apply vaccines to fish. However, mechanisms of antigen uptake and immune recognition on the mucosal surfaces of fish are largely unknown. We have demonstrated that bacterial vaccine antigens are taken up by gill epithelial antigen sampling (GAS) cells. In this study, we showed that teleost GAS cells have functions in antigen processing and antigen presentation. These results may contribute to develop a new technique for fish bath-vaccination.

Academic Significance and Societal Importance of the Research Achievements

浸漬ワクチンは、これまで限られた病原体に対してのみ使用可能であった。本研究では、鰓上皮抗原取込細胞によりワクチン成分が取り込まれるか否かにより、その浸漬ワクチンとしての有効性が担保される可能性を示した。今後、鰓上皮抗原取込細胞により取り込まれやすい細菌や取り込まれやすい分子を明らかにすることで、鰓上皮抗原取込細胞を標的とした新たな浸漬ワクチンの開発が期待される。

Research Products

• [Int'l Joint Research] Friedrich-Loeffler-Institut(ドイツ)
• [Int'l Joint Research] Friedrich-Loeffler-Institut(Germany)
• [Int'l Joint Research] Friedrich-Loeffler-Institut(ドイツ)
• [Journal Article] A Novel Antigen-Sampling Cell in the Teleost Gill Epithelium With the Potential for Direct Antigen Presentation in Mucosal Tissue (2018)
  • Author(s): Kato Goshi、Miyazawa Haruya、Nakayama Yumiko、Ikari Yuki、Kondo Hidehiro、Yamaguchi Takuya、Sano Motohiko、Fischer Uwe
  • Journal Title: Frontiers in Immunology Volume: 9 Pages: 2116-2116
  • DOI: 10.3389/fimmu.2018.02116
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] GILL-EPITHELIAL ANTIGEN SAMPLING CELLS IN RAINBOW TROUT (2018)
  • Author(s): Goshi Kato, Haruya Miyazawa, Yumiko Nakayama, Yuki Ikari, Hidehiro Kondo, Takuya Yamaguchi, Motohiko Sano & Uwe Fischer
  • Organizer: The 14th Congress of the International Society for Developmental and Comparative Immunology
  • Int'l Joint Research
• [Presentation] ニジマス鰓上皮抗原取込細胞に対するモノクローナル抗体の作出 (2017)
  • Author(s): 碇 由紀・市田健介・山口卓哉・Uwe Fischer・佐野元彦 ・加藤豪司
  • Organizer: 平成29年度日本魚病学会春季大会
  • Place of Presentation: 日本大学生物資源科学部
  • Year and Date: 2017-03-11
• [Presentation] Comprehensive gene expression analysis in gill-epithelium antigen sampling cells of rainbow trout. (2017)
  • Author(s): 2）Haruya Miyazawa, Uwe Fischer, Takuya Yamaguchi, Hidehiro Kondo, Motohiko Sano, Goshi Kato
  • Organizer: The JSFS 85th Anniversary-Commemorative International Symposium Fisheries Sciense for Future Generations
  • Int'l Joint Research
• [Presentation] MHC class II expression on gill epithelial antigen sampling cells. (2017)
  • Author(s): 4）Yumiko Nakayama, Takuya Yamaguchi, Uwe Fischer, Hidehiro Kondo, Motohiko Sano, Goshi Kato
  • Organizer: 10th Symposium on Diseases in Asian Aquaculture
  • Int'l Joint Research
• [Presentation] Development of a monoclonal antibody against gill epithelial antigen sampling cells of rainbow trout (2017)
  • Author(s): 6）Yuki Ikari, Kensuke Ichida, Takuya Yamaguchi, Uwe Fischer, Motohiko Sano, Goshi Kato
  • Organizer: 10th Symposium on Diseases in Asian Aquaculture
  • Int'l Joint Research