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Study on cold adaptation strategies and secondary metabolites of Antarctic yeast

Research Project

Project/Area Number 16H06211
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Environmental agriculture(including landscape science)
Research InstitutionNational Institute of Polar Research

Principal Investigator

TSUJI Masaharu  国立極地研究所, 研究教育系, 特任研究員 (70756923)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2018: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
Fiscal Year 2017: ¥8,970,000 (Direct Cost: ¥6,900,000、Indirect Cost: ¥2,070,000)
Fiscal Year 2016: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
Keywords南極 / 菌類 / 全ゲノム解析 / メタボローム 解析 / 二次代謝産物 / 低温適応 / メタボローム解析 / トランスクリプトーム解析
Outline of Final Research Achievements

The genus Mrakia is a dominant fungus around Syowa Station, East Antarctica. I chose this genus to study how this genus survives under the sub-zero temperature condition using by whole genome and metabolome analysis. Consequently, I found that Mrakia blollopis consumes large amounts of energy during growth to help it cope with cold stress as it grows in subzero temperatures.

I also checked the secondary metabolite having Antarctic fungi. It has become clear that Antarctic fungi are secreting many substances that are the raw materials of pharmaceuticals. From the above, it has become clear that Antarctic fungi are useful as bioprospecting source.

Academic Significance and Societal Importance of the Research Achievements

昭和基地周辺の菌類のうち、約35%を占めるMrakia属菌が極限環境下で生き抜くために細胞内でどのような遺伝子や代謝の応答を行っているのかを明らかにすることで、大陸性南極でも豊かな生態系が存在するとされている昭和基地周辺において、どのように生態系が維持されているのか?を解明する足がかりとなったと考えている。また、南極大陸は微生物資源調査における最後のフロンティアとされていることから、この地域に生息している菌類が持つ有用な二次代謝産物を積極的に探索したことで、将来的に様々な企業に大きなインパクトを与えることができると考えている。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (31 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (12 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 10 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (16 results) (of which Int'l Joint Research: 7 results,  Invited: 2 results) Patent(Industrial Property Rights) (1 results)

  • [Int'l Joint Research] ラバウル大学(カナダ)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] National centre for/Antarctic and Ocean Research(インド)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Mrakia hoshinonis sp. nov., a novel psychrophilic yeast isolated from a retreating glacier on Ellesmere Island in the Canadian High Arctic2019

    • Author(s)
      Tsuji Masaharu、Tanabe Yukiko、Vincent Warwick F.、Uchida Masaki
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 69 Issue: 4 Pages: 944-948

    • DOI

      10.1099/ijsem.0.003216

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Vishniacozyma ellesmerensis sp. nov., a psychrophilic yeast isolated from a retreating glacier in the Canadian High Arctic2019

    • Author(s)
      Tsuji Masaharu、Tanabe Yukiko、Vincent Warwick F.、Uchida Masaki
    • Journal Title

      International Journal of Systematic and Evolutionary Microbiology

      Volume: 69 Issue: 3 Pages: 696-700

    • DOI

      10.1099/ijsem.0.003206

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Gelidatrema psychrophila sp. nov., a novel yeast species isolated from an ice island in the Canadian High Arctic2018

    • Author(s)
      Tsuji Masaharu、Tanabe Yukiko、Vincent Warwick F.、Uchida Masaki
    • Journal Title

      Mycoscience

      Volume: 59 Issue: 1 Pages: 67-70

    • DOI

      10.1016/j.myc.2017.08.010

    • NAID

      40021424366

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Mrakia arctica sp. nov., a new psychrophilic yeast isolated from an ice island in the Canadian High Arctic2018

    • Author(s)
      Tsuji Masaharu、Tanabe Yukiko、Vincent Warwick F.、Uchida Masaki
    • Journal Title

      Mycoscience

      Volume: 59 Issue: 1 Pages: 54-58

    • DOI

      10.1016/j.myc.2017.08.006

    • NAID

      40021424331

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A catalog of fungi recorded from the vicinity of Syowa Station2018

    • Author(s)
      Tsuji Masaharu
    • Journal Title

      Mycoscience

      Volume: ー Issue: 4 Pages: 319-324

    • DOI

      10.1016/j.myc.2017.08.007

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genetic diversity of yeasts from East Ongul Island, East Antarctica and their extracellular enzymes secretion2017

    • Author(s)
      Tsuji Masaharu
    • Journal Title

      Polar biology

      Volume: 41 Issue: 2 Pages: 249-258

    • DOI

      10.1007/s00300-017-2185-1

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cystobasidium tubakii and Cystobasidium ongulense, new basidiomycetous yeast species isolated from East Ongul Island, East Antarctica2017

    • Author(s)
      Tsuji M, Tsujimoto M, Imura S
    • Journal Title

      Mycosicnece

      Volume: 58 Issue: 2 Pages: 103-110

    • DOI

      10.1016/j.myc.2016.11.002

    • NAID

      40021141371

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Characterization of yeast and filamentous fungi from Broggerbreen glaciers, Svalbard2016

    • Author(s)
      Singh P, Tsuji M, Roy U
    • Journal Title

      Polar record

      Volume: in press Issue: 4 Pages: 1-8

    • DOI

      10.1017/s0032247416000085

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cold-stress responses in the Antarctic basidiomycetous yeast Mrakia blollopis2016

    • Author(s)
      Tsuji M
    • Journal Title

      Royal Society Open Science

      Volume: 3 Issue: 7 Pages: 160106-160106

    • DOI

      10.1098/rsos.160106

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Changes in fungal community of Austre Broggerbreen deglaciation area, Ny-Alesund, Svalbard, High Arctic2016

    • Author(s)
      Tsuji M, Uetake J, Tanabe Y.
    • Journal Title

      Mycosicnece

      Volume: 57 Issue: 6 Pages: 448-451

    • DOI

      10.1016/j.myc.2016.07.006

    • NAID

      40021027242

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 南極産酵母の環境適応機構の 解明とその産業利用2016

    • Author(s)
      星野 保・辻 雅晴・横田 祐司・工藤 栄・内海 洋・湯本 勲
    • Journal Title

      生物工学会誌

      Volume: 94 Pages: 329-331

    • Related Report
      2016 Annual Research Report
  • [Journal Article] 南極湖沼より得られた菌類の性質とその産業利用2016

    • Author(s)
      星野 保・辻 雅晴・肖 楠・工藤 栄
    • Journal Title

      極地

      Volume: 52 Pages: 20-24

    • Related Report
      2016 Annual Research Report
  • [Presentation] Soil bacterial communities in Canadian subarctic tundra2018

    • Author(s)
      Kaneko R, Kiatagawa R, Nishizawa K, Masumoto S, Mori A, Uetake J, Tsuji M, Uchida M
    • Organizer
      第9回極域シンポジウム
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] カナダ高緯度北極 エルズミア島氷河後退域における菌類の多様性2018

    • Author(s)
      辻 雅晴、田邊 優貴子、WF Vincent, 内田 雅己
    • Organizer
      第9回極域シンポジウム
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 南北両極氷床コアに眠る菌類2018

    • Author(s)
      辻 雅晴, 中澤 文男, 伊村 智
    • Organizer
      第61回日本菌学会大会
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 極地に住む菌類とその排水処理、酒類醸造への試み2018

    • Author(s)
      辻 雅晴
    • Organizer
      サッポロビール研究集会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 極地に住む菌類とその排水処理、酒類醸造への利用”2018

    • Author(s)
      辻 雅晴
    • Organizer
      2018年日本生物工学会賀詞交換会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 低温ストレスにより誘導される南極産担子菌酵母が持つ二次代謝産物の探索2017

    • Author(s)
      辻 雅晴
    • Organizer
      日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] カナダ高緯度北極 エルズミア島における菌類の多様性2017

    • Author(s)
      辻 雅晴、田邊 優貴子、WF Vincent, 内田 雅己
    • Organizer
      第8回極域シンポジウム
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 南北両極氷床コアに眠る菌類2017

    • Author(s)
      辻 雅晴, 中澤 文男, 伊村 智
    • Organizer
      第8回極域シンポジウム
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 南極産担子菌酵母の環境適応とその産業利用2017

    • Author(s)
      星野 保、辻 雅晴、肖 楠、工藤 栄、湯本 勳
    • Organizer
      第18回極限生物学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] カナダ北極・エルズミーア島氷河後退域における菌類の多様性と南北両極アイスコアに眠る菌類の分離について2017

    • Author(s)
      辻 雅晴、田邊 優貴子、中澤 文男、WF Vincent, 内田 雅己
    • Organizer
      雪氷研究大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 南極産菌類の低温適応戦略の解明とそのバイオテクノロジーへの応用2017

    • Author(s)
      辻 雅晴
    • Organizer
      生物工学フォーラム
    • Related Report
      2017 Annual Research Report
  • [Presentation] Cold adaptation strategy of Antarctic basidiomycetous yeast2016

    • Author(s)
      Tsuji M
    • Organizer
      7th Symposium on Polar Science
    • Place of Presentation
      立川市
    • Year and Date
      2016-11-29
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 北極圏とアジア地域における雪氷藻類の種構成と色素の多様性2016

    • Author(s)
      中島智美、竹内望、植竹淳、田邊優貴子、辻雅晴、宮内謙史郎、岡本智夏
    • Organizer
      雪氷研究大会
    • Place of Presentation
      名古屋市
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] 南極産担子菌酵母Mrakia blollopisの低温ストレス反応2016

    • Author(s)
      辻 雅晴
    • Organizer
      日本生物工学会
    • Place of Presentation
      富山市
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Geographical variations in pigment compositions of snow algae in Japan2016

    • Author(s)
      Nakashima T, Takeuchi N, Uetake J, Tanabe Y, Tsuji M, Miyauchi K, Okamoto C
    • Organizer
      Goldschmidt
    • Place of Presentation
      横浜市
    • Year and Date
      2016-06-26
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 日本とアラスカの山岳地域に見られる赤雪の雪氷藻類の細胞形態と色素構成の比較2016

    • Author(s)
      中島智美、竹内望、田邊優貴子、辻雅晴、植竹淳、宮内謙史郎、岡本智夏
    • Organizer
      Japan Geoscience Union Meeting
    • Place of Presentation
      千葉市
    • Year and Date
      2016-05-20
    • Related Report
      2016 Annual Research Report
  • [Patent(Industrial Property Rights)] 南極産酵母による低温下でのエタノール製造方法2016

    • Inventor(s)
      星野保、辻雅晴、工藤栄
    • Industrial Property Rights Holder
      星野保、辻雅晴、工藤栄
    • Industrial Property Rights Type
      特許
    • Filing Date
      2016
    • Acquisition Date
      2018
    • Related Report
      2017 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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