| Budget Amount *help |
¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000)
Fiscal Year 2019: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2018: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
Fiscal Year 2017: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2016: ¥8,710,000 (Direct Cost: ¥6,700,000、Indirect Cost: ¥2,010,000)
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| Outline of Final Research Achievements |
The central nervous system (CNS) barriers including blood-brain barrier (BBB) is an important route to exchange a variety of substances including drugs between peripheral and CNS tissues. Transporting proteins expressed at the plasma membrane play important roles. The previous technologies and studies were not able to pursue the kinds, amounts and functions of transporting proteins at the plasma membrane, and mainly using rodents but not human materials. In the present study, we established comprehensive absolute quantitative proteomics “qGAP method” applicable for membrane proteins, and comprehensively elucidated new transport-molecular mechanisms in the CNS barriers including human BBB. The findings of many transporters (abundant expressions of OAT1, OAT3, MATE1, OCT2 etc) enabled to rationally understand the CNS pharmacokinetics. In conclusion, the present study was the first to systematically establish the basis of human CNS barrier biology.
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