| Budget Amount *help |
¥16,120,000 (Direct Cost: ¥12,400,000、Indirect Cost: ¥3,720,000)
Fiscal Year 2018: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2017: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2016: ¥8,060,000 (Direct Cost: ¥6,200,000、Indirect Cost: ¥1,860,000)
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| Outline of Final Research Achievements |
Type IV pili (T4P) are cell-surface appendages observed in prokaryotes that perform critical functions in cell motility, surface adhesion, virulence, and biofilm formation. Although the architecture of T4P has already been determined, the dynamics resulting from the response to various environmental signals remain unclear. Here we demonstrated the sequential process of T4P dynamics from stimulus to taxis at the single-cell level in a model cyanobacterium, which can recognize light direction. We directly visualized that T4P filaments dominantly appeared from the side of the cell opposite the illumination. This asymmetric activation is regulated on a timescale of minutes, and the process was transitioned between three sequential phases. These findings provide clues toward a general regulation mechanism of the T4P system.
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