| Budget Amount *help |
¥25,220,000 (Direct Cost: ¥19,400,000、Indirect Cost: ¥5,820,000)
Fiscal Year 2019: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2017: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
Fiscal Year 2016: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
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| Outline of Final Research Achievements |
Herpes simplex virus (HSV) is a common pathogen that causes skin infection as well as fatal encephalitis. HSV is categorized into 2 types: HSV type 1 (HSV-1) and HSV type 2 (HSV-2). Targeting host factors involved in HSV-infection and -propagation is an attractive approach to regulate those viruses. To identify a set of HSV host factors, we carried out a genome wide CRISPR screen. In this study, we established high quality KO library that showed the presentation of over 98% individual sgRNA. We isolated 53 genes for HSV-1, and 21 genes for HSV-2 as responsible host genes for viral infection and propagation. Among these isolated genes, NECTIN-1 and a series of genes involved in heparan sulfate biosynthesis, which are known for HSV viral receptors, were included. The other candidate genes are currently being investigated. Our study may provide new insights into the molecular mechanism of HSV viral entry and of the tropism that differs between HSV-1 and HSV-2.
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