| Budget Amount *help |
¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
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| Outline of Final Research Achievements |
Many of the pathogenic gene mutations are associated with characteristic metabolic changes. In this study, we developed a magnetic resonance imaging (MRI) technique that detects specific metabolic changes dependent on gene mutations by exciting the NMR signals of 13C-labeled biomolecules tens of thousands of times. We have succeeded in developing the hyperpolarization technique to enhance 13C NMR signals of biomolecules 50,000 to 100,000 times by using the hydrogenation of singlet state hydrogen gas, which can align quantum states relatively easily. Hyperpolarized 13C-pyruviate was administered to a tumor-bearing mouse, and production of lactate was confirmed according to the gene expression level of lactate dehydrogenase (LDH). MRI signal of 13C fumarate, which is used to detect gene mutations in the tumor suppressor genes fumarase and succinate dehydrogenase, could be enhanced more than 100,000 times, and succeeded to conduct metabolic MRI scan in mice.
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