• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Noninvasive imaging of acquired gene mutations using singlet state hydrogen gas.

Research Project

Project/Area Number 16H06255
Research Category

Grant-in-Aid for Young Scientists (A)

Allocation TypeSingle-year Grants
Research Field Radiation science
Research InstitutionHokkaido University

Principal Investigator

Matsumoto Shingo  北海道大学, 情報科学研究院, 准教授 (90741041)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2019: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2018: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2016: ¥11,830,000 (Direct Cost: ¥9,100,000、Indirect Cost: ¥2,730,000)
Keywords超偏極13C MRI / 遺伝子変異 / 代謝 / パラ水素誘起偏極 / MRI / 核偏極 / 分子イメージング / 計測工学 / 癌 / 遺伝子 / トランスレーショナルリサーチ
Outline of Final Research Achievements

Many of the pathogenic gene mutations are associated with characteristic metabolic changes. In this study, we developed a magnetic resonance imaging (MRI) technique that detects specific metabolic changes dependent on gene mutations by exciting the NMR signals of 13C-labeled biomolecules tens of thousands of times. We have succeeded in developing the hyperpolarization technique to enhance 13C NMR signals of biomolecules 50,000 to 100,000 times by using the hydrogenation of singlet state hydrogen gas, which can align quantum states relatively easily. Hyperpolarized 13C-pyruviate was administered to a tumor-bearing mouse, and production of lactate was confirmed according to the gene expression level of lactate dehydrogenase (LDH). MRI signal of 13C fumarate, which is used to detect gene mutations in the tumor suppressor genes fumarase and succinate dehydrogenase, could be enhanced more than 100,000 times, and succeeded to conduct metabolic MRI scan in mice.

Academic Significance and Societal Importance of the Research Achievements

多くの急性・慢性疾患に遺伝的要因が関与する。先天的に受け継いだ遺伝子多型や変異であれば血液検査等により容易に検出できるが、突然変異等により後天的に獲得した遺伝子変異は、体内の奥深く、特定の臓器や組織のみに偏在し、その特定には侵襲的な生検採取が必要となる。もし変異により起こる特徴的な代謝変化をMRIで検出できれば、非侵襲的に後天的遺伝子変異の種類、位置、量を知ることが可能となる。超偏極13C MRIは13C標識した任意の化合物の生体内代謝を動的に計測する最先端技術である。本研究成果は、非侵襲的遺伝子診断としての価値に加え、数億円に上る現行の超偏極13C MRI導入コストの低減にも繋がる。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Annual Research Report
  • Research Products

    (16 results)

All 2021 2019 2018 2017 2016 Other

All Int'l Joint Research (2 results) Journal Article (4 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 4 results,  Open Access: 2 results) Presentation (9 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results) Remarks (1 results)

  • [Int'l Joint Research] National Cancer Institute, NIH(米国)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] National Cancer Institute (NCI/NIH)(米国)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Biomedical Applications of the Dynamic Nuclear Polarization and Parahydrogen Induced Polarization Techniques for Hyperpolarized <sup>13</sup>C MR Imaging2021

    • Author(s)
      Stewart NJ, Matsumoto S.
    • Journal Title

      Magnetic Resonance in Medical Sciences

      Volume: 20 Issue: 1 Pages: 1-17

    • DOI

      10.2463/mrms.rev.2019-0094

    • NAID

      130007993571

    • ISSN
      1347-3182, 1880-2206
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Macrophage derived TNFα promotes hepatic reprogramming to Warburg-like metabolism.2019

    • Author(s)
      Tarasenko TN, Jestin M, Matsumoto S, Saito K, Hwang S, Gavrilova O, Trivedi N, Zerfas PM, Barca E, DiMauro S, Senac J, Venditti CP, Cherukuri M, McGuire PJ
    • Journal Title

      Journal of Molecular Medicine

      Volume: 97 Issue: 9 Pages: 1231-1243

    • DOI

      10.1007/s00109-019-01786-w

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Synthesis and evaluation of 13C-labeled 5-5-dimethyl-1-pyrroline-N-oxide aimed at in vivo detection of reactive oxygen species using hyperpolarized 13C-MRI.2019

    • Author(s)
      Saito K, Sail D, Yamamoto K, Matsumoto S, Blackman B, Kishimoto S, Brender JR, Swenson RE, Mitchell JB, Krishna MC.
    • Journal Title

      Free Radical Biology and Medicine

      Volume: 131 Pages: 18-26

    • DOI

      10.1016/j.freeradbiomed.2018.11.013

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Long-range heteronuclear J-coupling constants in esters: Implications for 13C metabolic MRI by side-arm parahydrogen-induced polarization.2018

    • Author(s)
      Stewart NJ, Kumeta H, Tomohiro M, Hashimoto T, Hatae N, Matsumoto S.
    • Journal Title

      J Magn Reson.

      Volume: 296 Pages: 85-92

    • DOI

      10.1016/j.jmr.2018.08.009

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] テンソル分解による超偏極13C代謝MRI画像からのノイズ除去法の検討2019

    • Author(s)
      辻野正之介、Neil J. Stewart、平田拓、松元慎吾
    • Organizer
      第14回 日本分子イメージング学会 総会・学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 深層学習を利用した超偏極13C MRIの高速撮像法の開発2019

    • Author(s)
      菅井秀斗、Neil J. Stewart、松元慎吾
    • Organizer
      第14回 日本分子イメージング学会 総会・学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Parahydrogen induced hyperpolarization of [1-13C] pyruvate for metabolic imaging in cancer research2019

    • Author(s)
      Shingo Matsumoto, Neil J. Stewart, Yuka Fukue, Hiroshi Hirata
    • Organizer
      ISMRM 2019
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] DDSにおける核偏極MRI技術の可能性2019

    • Author(s)
      松元慎吾
    • Organizer
      第35回日本DDS学会学術集会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Partial k space chemical shift imaging approach optimized for hyperpolarized 13C MRI2018

    • Author(s)
      Shuto Sugai , Neil J Stewart , Shingo Matsumoto
    • Organizer
      日本磁気共鳴医学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 核偏極の代謝イメージングへの応用2017

    • Author(s)
      松元慎吾
    • Organizer
      第72回日本物理学会年次大会
    • Place of Presentation
      大阪大学(大阪府・豊中市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 磁気共鳴による酸素イメージングの癌治療への応用2017

    • Author(s)
      松元慎吾
    • Organizer
      第5回 低酸素研究会
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] PHIP型の超偏極13C MRI代謝イメージングシステムの構築2016

    • Author(s)
      内尾佳貴、平田拓、松元慎吾
    • Organizer
      第44回日本磁気共鳴医学会大会
    • Place of Presentation
      大宮ソニックシティ(埼玉県・大宮市)
    • Year and Date
      2016-09-09
    • Related Report
      2016 Annual Research Report
  • [Presentation] 超偏極13C MRI代謝イメージングのためのパラ水素誘起分極(PHIP)型13C励起装置の開発2016

    • Author(s)
      中西沙由里、平田拓、松元慎吾
    • Organizer
      第44回日本磁気共鳴医学会大会
    • Place of Presentation
      大宮ソニックシティ(埼玉県・大宮市)
    • Year and Date
      2016-09-09
    • Related Report
      2016 Annual Research Report
  • [Remarks] 北海道大学 大学院情報科学研究院 磁気共鳴工学研究室 核偏極MRIチーム

    • URL

      https://hp13c-mri.com

    • Related Report
      2019 Annual Research Report

URL: 

Published: 2016-04-21   Modified: 2022-01-28  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi