| Budget Amount *help |
¥23,920,000 (Direct Cost: ¥18,400,000、Indirect Cost: ¥5,520,000)
Fiscal Year 2019: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2016: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
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| Outline of Final Research Achievements |
This study was performed to classify the phenotype of pancreatic cancer organoids derived from human pancreatic tissue and to clarify the difference in the extracellular matrix (ECM) remodeling ability of activated pancreatic stellate cells (PSCs) depending on the phenotypes. The organoids maintained the morphology of the pancreatic tissues, and phenotype classification was possible based on the niche factors dependency and the tumor differentiation. We established a three-dimensional co-culture model using the organoids and PSCs, and observed how the pancreatic cancer cells invade the ECM. These revealed that the basement membrane destruction by PSCs via MMP2 and MT1-MMP. In the future, we challenge to elucidate the differences in ECM remodeling by PSCs depending on each pancreatic cancer phenotype and to develop a personalized treatment that controls cancer cell invasion.
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