| Budget Amount *help |
¥24,310,000 (Direct Cost: ¥18,700,000、Indirect Cost: ¥5,610,000)
Fiscal Year 2018: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2017: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2016: ¥12,610,000 (Direct Cost: ¥9,700,000、Indirect Cost: ¥2,910,000)
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| Outline of Final Research Achievements |
To understand cementogenesis, we aimed to identify cementoblast specific marker. Using laser microdissection of cementum layer in histological section, we isolated RNA and protein for RNA-seq and proteome analysis, respectively, and found out candidates genes for cementoblast marker. Then, we established clonal periodontal ligament cell lines with a new primary culture protocol for murine periodontal ligament. Functional analysis of the genes were done by either CRISPR/Cas9 or lentiviral vector system. We also generated new knock-in mouse to understand cementoblasts in vivo.
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