| Budget Amount *help |
¥173,550,000 (Direct Cost: ¥133,500,000、Indirect Cost: ¥40,050,000)
Fiscal Year 2020: ¥21,710,000 (Direct Cost: ¥16,700,000、Indirect Cost: ¥5,010,000)
Fiscal Year 2019: ¥22,490,000 (Direct Cost: ¥17,300,000、Indirect Cost: ¥5,190,000)
Fiscal Year 2018: ¥45,500,000 (Direct Cost: ¥35,000,000、Indirect Cost: ¥10,500,000)
Fiscal Year 2017: ¥44,200,000 (Direct Cost: ¥34,000,000、Indirect Cost: ¥10,200,000)
Fiscal Year 2016: ¥39,650,000 (Direct Cost: ¥30,500,000、Indirect Cost: ¥9,150,000)
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| Outline of Final Research Achievements |
This project revealed that, during the intoation of mammalian nucleotide excision repair (NER), histone deacetylation around DNA damage sites promotes the recruitment of XPC protein, thereby contributing to the enhancement of DNA repair efficiency. Even in the absence of DNA damage, XPC could be recruited by HDACs tethered on specific genomic sites. Roles of protein degradation by the ubiquitin-proteasome system were also uncovered in the molecular mechanism, which facilitates the UV-DDB-dependent process of DNA lesion recognition. In addition, it was elucidated that UV-DDB (DDB1-DDB2 heterodimer) induces alteration of the nucleosome structure, by which DNA lesions buried within the nucleosome core are exposed outward and allowed to interact with UV-DDB.
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