| Budget Amount *help |
¥177,580,000 (Direct Cost: ¥136,600,000、Indirect Cost: ¥40,980,000)
Fiscal Year 2020: ¥29,250,000 (Direct Cost: ¥22,500,000、Indirect Cost: ¥6,750,000)
Fiscal Year 2019: ¥31,850,000 (Direct Cost: ¥24,500,000、Indirect Cost: ¥7,350,000)
Fiscal Year 2018: ¥27,560,000 (Direct Cost: ¥21,200,000、Indirect Cost: ¥6,360,000)
Fiscal Year 2017: ¥50,830,000 (Direct Cost: ¥39,100,000、Indirect Cost: ¥11,730,000)
Fiscal Year 2016: ¥38,090,000 (Direct Cost: ¥29,300,000、Indirect Cost: ¥8,790,000)
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| Outline of Final Research Achievements |
By integrating microfluidics and CMOS technologies, a novel microfluidic system has been developed for trapping single cells and/or microparticles into designated locations, followed by parallelized analysis of trapped single cells. Moreover, a new protocol for cap analysis of gene expression has been established by utilizing combinatorial DNA barcodes for efficient labeling of single cells. By using these methods, clinical samples from cervical cancer patients were successfully analyzed, and it was confirmed that the cervical cancer could be detected by analyzing RNA or protein expression level.
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