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Molecular mechanism and physiological understanding of Autophagy

Research Project

Project/Area Number 16H06375
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Cell biology
Research InstitutionTokyo Institute of Technology

Principal Investigator

Ohsumi Yoshinori  東京工業大学, 科学技術創成研究院, 栄誉教授 (30114416)

Co-Investigator(Kenkyū-buntansha) 堀江 朋子 (川俣朋子)  東京工業大学, 科学技術創成研究院, 助教 (70435527)
Project Period (FY) 2016-05-31 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥186,810,000 (Direct Cost: ¥143,700,000、Indirect Cost: ¥43,110,000)
Fiscal Year 2020: ¥16,250,000 (Direct Cost: ¥12,500,000、Indirect Cost: ¥3,750,000)
Fiscal Year 2019: ¥37,050,000 (Direct Cost: ¥28,500,000、Indirect Cost: ¥8,550,000)
Fiscal Year 2018: ¥34,580,000 (Direct Cost: ¥26,600,000、Indirect Cost: ¥7,980,000)
Fiscal Year 2017: ¥35,360,000 (Direct Cost: ¥27,200,000、Indirect Cost: ¥8,160,000)
Fiscal Year 2016: ¥63,570,000 (Direct Cost: ¥48,900,000、Indirect Cost: ¥14,670,000)
Keywordsオートファジー / 分解基質 / RNA分解 / 酵母 / ATG / タンパク質 / 細胞内分解 / 基質 / タンパク質分解 / 質量分析 / 膜脂質 / 膜動態 / ユビキチン様タンパク質
Outline of Final Research Achievements

Research on autophagy has been widespread, but it still has the drawback of weak biochemical verification. The purpose of this study was to clarify what, when and how cytoplasmic component are degraded by autophagy. Taking advantage of the yeast system, which has accumulated a lot of knowledge, we have established a new method and gave a new perspective on the accurate grasp of degradation targets and final degradation products and their effects on cell metabolism.

Academic Significance and Societal Importance of the Research Achievements

オートファジー研究は、今日哺乳動物における生理的役割に関して目覚ましい展開を見ているが、その分子レベルの理解に関しては、多くのことがブラックボックスとして残されている。本研究は30年以上の酵母の系での実績と、系の優位性を生かし、分解基質、分解過程、分解産物の解析を通じて、今一度高等動植物のオートファジー研究に新しい視点を与えることを目指してきたが、一定の成果をあげることができたと考えている。

Assessment Rating
Verification Result (Rating)

A

Assessment Rating
Result (Rating)

A: Progress in the research is steadily towards the initial goal. Expected research results are expected.

Report

(11 results)
  • 2021 Research Progress Assessment (Verification Result) ( PDF )
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Annual Research Report   Abstract(Research Progress Assessment) ( PDF )   Research Progress Assessment (Result) ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Abstract ( PDF )   Comments on the Screening Results ( PDF )   Annual Research Report
  • Research Products

    (49 results)

All 2020 2019 2018 2017 2016 Other

All Journal Article (20 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 19 results,  Open Access: 17 results,  Acknowledgement Compliant: 2 results) Presentation (27 results) (of which Int'l Joint Research: 26 results,  Invited: 26 results) Remarks (2 results)

  • [Journal Article] Autophagy increases zinc bioavailability to avoid light-mediated reactive oxygen species production under zinc deficiency.2020

    • Author(s)
      6.Shinozaki D, Merkulova A. E, Naya L, Horie T, Kanno Y, Seo M, Ohsumi Y, Masclaux-Daubresse C, *Yoshimoto K.
    • Journal Title

      Plant Physiol.

      Volume: 182 Issue: 3 Pages: 1284-296

    • DOI

      10.1104/pp.19.01522

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Liquidity Is a Critical Determinant for Selective Autophagy of Protein Condensates2020

    • Author(s)
      Yamasaki Akinori、Alam Jahangir Md.、Noshiro Daisuke、Hirata Eri、Fujioka Yuko、Suzuki Kuninori、Ohsumi Yoshinori、Noda Nobuo N.
    • Journal Title

      Molecular Cell

      Volume: 77 Issue: 6 Pages: 1163-1175

    • DOI

      10.1016/j.molcel.2019.12.026

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phase separation organizes the site of autophagosome formation2020

    • Author(s)
      Fujioka Yuko、Alam Jahangir Md.、Noshiro Daisuke、Mouri Kazunari、Ando Toshio、Okada Yasushi、May Alexander I.、Knorr Roland L.、Suzuki Kuninori、Ohsumi Yoshinori、Noda Nobuo N.
    • Journal Title

      Nature

      Volume: 578 Issue: 7794 Pages: 301-305

    • DOI

      10.1038/s41586-020-1977-6

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] TORC1 inactivation stimulates autophagy of nucleoporin and nuclear pore complexes2020

    • Author(s)
      Tomioka Yui、Kotani Tetsuya、Kirisako Hiromi、Oikawa Yu、Kimura Yayoi、Hirano Hisashi、Ohsumi Yoshinori、Nakatogawa Hitoshi
    • Journal Title

      Journal of Cell Biology

      Volume: 219 Issue: 7 Pages: 301-305

    • DOI

      10.1083/jcb.201910063

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autophagy facilitates adaptation of budding yeast to respiratory growth by recycling serine for one-carbon metabolism2020

    • Author(s)
      May Alexander I.、Prescott Mark、Ohsumi Yoshinori
    • Journal Title

      Nature Communications

      Volume: 11 Issue: 1

    • DOI

      10.1038/s41467-020-18805-x

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Atg9 is a lipid scramblase that mediates autophagosomal membrane expansion2020

    • Author(s)
      Matoba Kazuaki、Kotani Tetsuya、Tsutsumi Akihisa、Tsuji Takuma、Mori Takaharu、Noshiro Daisuke、Sugita Yuji、Nomura Norimichi、Iwata So、Ohsumi Yoshinori、Fujimoto Toyoshi、Nakatogawa Hitoshi、Kikkawa Masahide、Noda Nobuo N.
    • Journal Title

      Nature Structural & Molecular Biology

      Volume: 27 Issue: 12 Pages: 1185-1193

    • DOI

      10.1038/s41594-020-00518-w

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Atg2 mediates direct lipid transfer between membranes for autophagosome formation.2019

    • Author(s)
      Osawa T, Kotani T, Kawaoka T, Hirata E, Suzuki K, Nakatogawa H, Ohsumi Y, Noda NN*.
    • Journal Title

      Nat. Struct. Mol. Biol.

      Volume: 26 Pages: 281-288

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Two distinct mechanisms target the autophagy-related E3 complex to the pre-autophagosomal structure2019

    • Author(s)
      Harada K**, Kotani T**, Kirisako H, Sakoh-Nakatogawa M, Oikawa Y, Kimura Y, Hirano H, Yamamoto H, Ohsumi Y, Nakatogawa H*.,
    • Journal Title

      eLife

      Volume: 8

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of autophagy activated during changes in carbon source availability in yeast cells2019

    • Author(s)
      Iwama R, Ohsumi Y.
    • Journal Title

      J. Biol. Chem.

      Volume: 294 Pages: 5590-5603

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of autophagy activated during changes in carbon source availability in yeast cells.2019

    • Author(s)
      Iwama R, Ohsumi Y.
    • Journal Title

      J. Biol. Chem.

      Volume: 294 Pages: 5590-5603

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Atg2-Atg18 complex tethers pre-autophagosomal membranes to the endoplasmic reticulum for autophagosome formation.2018

    • Author(s)
      Kotani T, Kirisako H, Koizumi M, Ohsumi Y, Nakatogawa H.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 115 Pages: 10363-10368

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Lipidation-independent vacuolar functions of Atg8 rely on its noncanonical interaction with a vacuole membrane protein2018

    • Author(s)
      Liu XM, Yamasaki A, Du XM, Coffman VC, Ohsumi Y, Nakatogawa H, Wu JQ, Noda NN.
    • Journal Title

      eLIFE

      Volume: 7

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Atg7 activates an autophagy-essential ubiquitin-like protein Atg8 through multi-step recognition2018

    • Author(s)
      Yamaguchi M, Satoo K, Suzuki H, Fujioka Y, Ohsumi Y, Inagaki F, Noda NN.
    • Journal Title

      J. Mol. Biol.

      Volume: 430 Pages: 249-257

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Autophagy induction under carbon starvation conditions is negatively regulated by carbon catabolite repression2017

    • Author(s)
      Adachi A, Koizumi M, Ohsumi Y.
    • Journal Title

      J. Biol. Chem.

      Volume: 292 Pages: 19905-19918

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recycling of iron via autophagy is critical for the transition from glycolytic to respiratory growth2017

    • Author(s)
      Horie T, Kawamata T, Matsunami M, Ohsumi Y.
    • Journal Title

      J. Biol. Chem.[Faculty of 1000]

      Volume: 292 Pages: 8533-8543

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Zinc starvation induces autophagy in yeast2017

    • Author(s)
      Kawamata T, Horie T, Matsunami M, Sasaki M, Ohsumi Y.
    • Journal Title

      J. Biol. Chem.[editor’s pick up]

      Volume: 292 Pages: 8520-8530

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Recycling of trace metals by the bulk autophagy in the budding yeast, Saccharomyces cerevisiae2017

    • Author(s)
      Horie T, Kawamata T, Ohsumi Y.
    • Journal Title

      J. Adv. Nutr. Hum. Metab.

      Volume: 3

    • Related Report
      2017 Annual Research Report
    • Open Access
  • [Journal Article] Functional identification of AtAVT3, a family of vacuolar amino acid transporters, from Arabidopsis.2017

    • Author(s)
      Fujiki Y, Teshima H, Kashiwao S, Kawano-Kawada M, Ohsumi Y, Kakinuma Y and Sekito T
    • Journal Title

      FEBS Letters

      Volume: 591 Issue: 1 Pages: 5-15

    • DOI

      10.1002/1873-3468.12507

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] The Intrinsically Disordered Protein Atg13 Mediates Supramolecular Assembly of Autophagy Initiation Complexes.2016

    • Author(s)
      Yamamoto H, Fujioka Y, Suzuki SW, Noshiro D, Suzuki H, Kondo-Kakuta C, Kimura Y, Hirano H, Ando T, Noda NN, Ohsumi Y
    • Journal Title

      Developmental Cell

      Volume: 38 Issue: 1 Pages: 86-99

    • DOI

      10.1016/j.devcel.2016.06.015

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structural mechanisms of receptor-mediated selective autophagy of aggregated aminopeptidase I.2016

    • Author(s)
      Akinori Yamasaki, Yasunori Watanabe, Wakana Adachi, Kuninori Suzuki, Kazuaki Matoba, Hiromi Kirisako, Hiroyuki Kumeta, Hitoshi Nakatogawa, Yoshinori Ohsumi, Fuyuhiko Inagaki and Nobuo N. Noda
    • Journal Title

      Cell Reports

      Volume: 16 Issue: 1 Pages: 19-27

    • DOI

      10.1016/j.celrep.2016.05.066

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] Lecture on Autophagy2020

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      The 'Healthy Moscow’ Assembly, Moscow
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Lessons from yeast : Molecular dissection of autophagy2019

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      NOBEL AROSS CHINA
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Recycling System : Essential For Life And Also For Society2019

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      the 49th St. Gallen Symposium, Switzerland
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Looking back on my 30 years’ of yeast autophagy research2019

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      30th KSMCB, Korea
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Lecture on Autophagy2019

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      The WLA 2nd annual Forum is organized by Shanghai Municipal Government
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Looking back on 30 years of autophagy research What have we learned from yeast?2019

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      9th International Symposium on Autophagy (9th ISA) in Taiwan
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Looking back on 30 years of autophagy research - dynamic equilibrium of the cell -2019

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      FAOPS(Federation of Asia Oceania Physiological Sciences)
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Lessons from Yeast - as a Cellular Recycling System2018

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      Cold Spring Harbor Laboratory Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Lessons from Yeast - Autophagy : Intracellular Recycling System2018

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      Lindau Nobel Laureate Meeting
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular details of degradation via autophagy in yeast-502018

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      International Symposium “Proteins: From the Cradle to the Grave”
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Lessons from Yeast - Autophagy as a Cellular Recycling System -2018

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      SIOP2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Lessons from Yeast - Autophagy as a Cellular Recycling System -2018

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      the CBIS conference
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Looking back on my 28 years of autophagy research2018

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      Nobel Prize Dialog, Yokohama, Japan
    • Related Report
      2017 Annual Research Report
  • [Presentation] Looking back on my 28 years of autophagy studies2017

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      the George E. Palade Lecture, Yale Univ. USA
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Lessons from Yeast - Autophagy as a Cellular Recycling System -2017

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      ISA(International Symposium on Autophagy), Nara, Japan
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular mechanism and physiological roles of autophagy2017

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      Yeast 2017, Prague, Czech Republic
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular mechanism and physiological roles of autophagy2017

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      The first Chinese Conference on Autophagy, Dairen, China
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Looking back on a half-century research career2017

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      ConBio2017, Looking back on a half-century research career, Yokohama, Japan
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Looking back on my 27 years of autophagy research2016

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      CSHL meeting, Asia
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular Machinery of Autophagy, Intracellular Recycling System2016

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      Rosenstiel Award, Brandies Univ.
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 27 Years’ Progress and Future Perspectives of Autophagy Research in Yeast2016

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      Wiley Prize, Rockefeller Univ.
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Looking back on my 27 years of autophagy research2016

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      SINO-JAPAN Autophagy Symposium
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 27 Years’ Progress and Future Perspective in Autophagy Research in Yeast2016

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      KSBMB 2016, Korea
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 27 Years’ Progress and Future Perspective in Autophagy Research in Yeast2016

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      Paul Janssen Award Symposium
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Molecular Machinery of Autophagy, Intracellular Recycling System2016

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      Milstein Lecture, Cambridge, MRC,UK
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Looking back on my 27 years of autophagy research2016

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      ICY16, Awaji, Japan
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Autophagy- an intracellular recycling system-2016

    • Author(s)
      Yoshinori Ohsumi
    • Organizer
      The Nobel Prize Lecture
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Remarks] オートファゴソームに脂質を供給する仕組みを解明~オートファジーにまつわる数十年来の謎が明らかに~

    • URL

      https://www.titech.ac.jp/news/pdf/tokyotechpr20190322_osumi_rTHoeker.pdf

    • Related Report
      2019 Annual Research Report
  • [Remarks] オートファジーは凝集体でなく液滴状態のたんぱく質を分解する 細胞内の「ゴミ」は溜まる前の処理が大事

    • URL

      https://www.titech.ac.jp/news/2020/046184.html

    • Related Report
      2019 Annual Research Report

URL: 

Published: 2016-06-01   Modified: 2023-10-16  

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