| Budget Amount *help |
¥177,840,000 (Direct Cost: ¥136,800,000、Indirect Cost: ¥41,040,000)
Fiscal Year 2020: ¥26,260,000 (Direct Cost: ¥20,200,000、Indirect Cost: ¥6,060,000)
Fiscal Year 2019: ¥36,400,000 (Direct Cost: ¥28,000,000、Indirect Cost: ¥8,400,000)
Fiscal Year 2018: ¥33,020,000 (Direct Cost: ¥25,400,000、Indirect Cost: ¥7,620,000)
Fiscal Year 2017: ¥32,110,000 (Direct Cost: ¥24,700,000、Indirect Cost: ¥7,410,000)
Fiscal Year 2016: ¥50,050,000 (Direct Cost: ¥38,500,000、Indirect Cost: ¥11,550,000)
|
|---|
| Outline of Final Research Achievements |
Newt can regenerate complete limb structures after amputation including functional joint regeneration. Newt limb regeneration is achieved by two steps, distalization and intercalation. We are trying to induce limb-regeneration ability in frogs and mice. In the case of frogs, they lose the capability to achieve complete limb regeneration after metamorphosis due to the loss of MFCS1 activity after metamorphosis, although distalization is induced. When we compared the MFCS1 sequences between newts and frogs, newts were found to possess several specific sequences. Thus, we subsequently swapped the MFCS1 sequences between newts and frogs using CRISPR/Cas9 technology to evoke limb regeneration in frogs. Although we have not yet succeeded in swapping the MFCS1 sequences, we have succeeded in evoking joint regeneration in mice by artificially inducing distalization.
|
