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Spatiotemporal regulation of cell division axis as a grand plan of plant developmental evolution

Research Project

Project/Area Number 16H06378
Research Category

Grant-in-Aid for Scientific Research (S)

Allocation TypeSingle-year Grants
Research Field Evolutionary biology
Research InstitutionNational Institute for Basic Biology

Principal Investigator

Hasebe Mitsuyasu  基礎生物学研究所, 生物進化研究部門, 教授 (40237996)

Co-Investigator(Kenkyū-buntansha) 村田 隆  神奈川工科大学, 応用バイオ科学部, 教授 (00242024)
石川 雅樹  基礎生物学研究所, 生物進化研究部門, 助教 (00586894)
望月 敦史  京都大学, ウイルス・再生医科学研究所, 教授 (10304726)
関本 弘之  日本女子大学, 理学部, 教授 (20281652)
小藤 累美子  金沢大学, 生命理工学系, 助教 (40324066)
藤本 仰一  大阪大学, 理学研究科, 准教授 (60334306)
玉田 洋介  宇都宮大学, 工学部, 准教授 (50579290)
Project Period (FY) 2016-05-31 – 2021-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥195,130,000 (Direct Cost: ¥150,100,000、Indirect Cost: ¥45,030,000)
Fiscal Year 2020: ¥19,760,000 (Direct Cost: ¥15,200,000、Indirect Cost: ¥4,560,000)
Fiscal Year 2019: ¥28,730,000 (Direct Cost: ¥22,100,000、Indirect Cost: ¥6,630,000)
Fiscal Year 2018: ¥24,960,000 (Direct Cost: ¥19,200,000、Indirect Cost: ¥5,760,000)
Fiscal Year 2017: ¥24,180,000 (Direct Cost: ¥18,600,000、Indirect Cost: ¥5,580,000)
Fiscal Year 2016: ¥97,500,000 (Direct Cost: ¥75,000,000、Indirect Cost: ¥22,500,000)
Keywordsヒメツリガネゴケ / 発生進化 / GRAS転写因子 / 局所細胞伸長 / ABC輸送体 / クチクラ / 細胞分裂軸 / ミカヅキモ / 分裂面形成 / 細胞分裂面制御 / 微小管
Outline of Final Research Achievements

In both animals and plants, proper regulation of cell division orientations is crucial for developmental processes. Changes in the regulatory mechanisms have contributed significantly to the evolution of body plans in multicellular organisms. It has been predicted that land plants have a different cell division axis determination mechanism from animals, but the molecular mechanism has not been well elucidated. In this study, we clarified the mechanism of spatio-temporal regulation of the GRAS transcription factors, which regulate the cell division axis, and found that the cell division axis is likely to be regulated by a novel mechanism of local cell elongation through the regulation of cuticular extension by ABC transporters.

Academic Significance and Societal Importance of the Research Achievements

植物の体は、細胞が積み重なってできている。細胞が分裂すると、分裂した部分は細胞の数が増えるので大きく育つ。また、細胞が分裂する方向によって、育つ方向が変わるので、体のどの位置の細胞が、どの方向に割れて増えるかによって、形が変化する。従って、植物の形は、細胞の分裂方向を制御する遺伝子が変化することで、多様に進化してきた。本研究では、3つの遺伝子が相互作用することによって決まった場所で決まった方向に細胞分裂させる仕組みを明らかにした。さらに、細胞分裂方向を決める新しい遺伝子と仕組みを発見した。

Assessment Rating
Verification Result (Rating)

A

Assessment Rating
Result (Rating)

A: Progress in the research is steadily towards the initial goal. Expected research results are expected.

Report

(11 results)
  • 2022 Research Progress Assessment (Verification Result) ( PDF )
  • 2021 Final Research Report ( PDF )
  • 2020 Annual Research Report
  • 2019 Annual Research Report   Abstract(Research Progress Assessment) ( PDF )   Research Progress Assessment (Result) ( PDF )
  • 2018 Annual Research Report
  • 2017 Annual Research Report
  • 2016 Abstract ( PDF )   Comments on the Screening Results ( PDF )   Annual Research Report
  • Research Products

    (46 results)

All 2022 2021 2020 2019 2018 2017 2016 Other

All Int'l Joint Research (8 results) Journal Article (15 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 15 results,  Open Access: 10 results) Presentation (21 results) (of which Int'l Joint Research: 10 results,  Invited: 11 results) Remarks (2 results)

  • [Int'l Joint Research] Duke University(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] 華中農業大学(中国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] チェコ科学アカデミー(チェコ)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Freiburg大学(ドイツ)

    • Related Report
      2019 Annual Research Report
  • [Int'l Joint Research] Freiburg University(ドイツ)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] Ghent University(ベルギー)

    • Related Report
      2017 Annual Research Report
  • [Int'l Joint Research] Tel-Aviv University(イスラエル)

    • Related Report
      2016 Annual Research Report
  • [Int'l Joint Research] University of Freiburg(ドイツ)

    • Related Report
      2016 Annual Research Report
  • [Journal Article] Topoisomerase 1α is required for synchronous spermatogenesis in Physcomitrium patens2022

    • Author(s)
      Nan Gu、Chunli Chen、Yukiko Kabeya、Mitsuyasu Hasebe、Yosuke Tamada
    • Journal Title

      New Phytologist

      Volume: 234 Issue: 1 Pages: 137-148

    • DOI

      10.1111/nph.17983

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Low-invasive 5D visualization of mitotic progression by two-photon excitation spinning-disk confocal microscopy2022

    • Author(s)
      Kamada Takafumi、Otomo Kohei、Murata Takashi、Nakata Kaito、Hiruma Shota、Uehara Ryota、Hasebe Mitsuyasu、Nemoto Tomomi
    • Journal Title

      Scientific Reports

      Volume: 12 Issue: 1 Pages: 1-10

    • DOI

      10.1038/s41598-021-04543-7

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Rotation angle of stem cell division plane controls spiral phyllotaxis in mosses.2021

    • Author(s)
      Kamamoto, N., Tano, T., Fujimoto, K. & Shimamura, M.
    • Journal Title

      Journal of Plant Research

      Volume: 134 Issue: 3 Pages: 457-473

    • DOI

      10.1007/s10265-021-01298-0

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Perianth Phyllotaxis Is Polymorphic in the Basal Eudicot Anemone and Eranthis Species2020

    • Author(s)
      Kitazawa Miho S.、Fujimoto Koichi
    • Journal Title

      Frontiers in Ecology and Evolution

      Volume: 8 Pages: 70-70

    • DOI

      10.3389/fevo.2020.00070

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A design principle for floral organ number and arrangement in flowers with bilateral symmetry2020

    • Author(s)
      Nakagawa Aiko、Kitazawa Miho S.、Fujimoto Koichi
    • Journal Title

      Development

      Volume: 147 Issue: 3

    • DOI

      10.1242/dev.182907

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] DNA damage triggers reprogramming of differentiated cells into stem cells in Physcomitrella2020

    • Author(s)
      Gu Nan、Tamada Yosuke、Imai Akihiro、Palfalvi Gergo、Kabeya Yukiko、Shigenobu Shuji、Ishikawa Masaki、Angelis Karel J.、Chen Chunli、Hasebe Mitsuyasu
    • Journal Title

      Nature Plants

      Volume: 6 Issue: 9 Pages: 1098-1105

    • DOI

      10.1038/s41477-020-0745-9

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Two ANGUSTIFOLIA genes regulate gametophore and sporophyte development in Physcomitrella patens2020

    • Author(s)
      Hashida Yoshikazu、Takechi Katsuaki、Abiru Tomomi、Yabe Noriyuki、Nagase Hiroaki、Hattori Koro、Takio Susumu、Sato Yoshikatsu、Hasebe Mitsuyasu、Tsukaya Hirokazu、Takano Hiroyoshi
    • Journal Title

      The Plant Journal

      Volume: 101 Issue: 6 Pages: 1318-1330

    • DOI

      10.1111/tpj.14592

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Single-cell transcriptome analysis of Physcomitrella leaf cells during reprogramming using microcapillary manipulation2019

    • Author(s)
      Minoru Kubo, Tomoaki Nishiyama, Yosuke Tamada, Ryosuke Sano, Masaki Ishikawa, Takashi Murata, Akihiro Imai, Daniel Lang, Taku Demura, Ralf Reski, Mitsuyasu Hasebe
    • Journal Title

      Nucleic Acid Research

      Volume: in press Issue: 9 Pages: 4539-4553

    • DOI

      10.1093/nar/gkz181

    • Related Report
      2019 Annual Research Report 2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Physcomitrella STEMIN transcription factor induces stem cell formation with epigenetic reprogramming2019

    • Author(s)
      Masaki Ishikawa, Mio Morishita, Yohei Higuchi, Shunsuke Ichikawa, Takaaki Ishikawa, Tomoaki Nishiyama, Yukiko Kabeya, Yukiko, Yuji Hiwatashi, Tetsuya Kurata, Minoru Kubo, Shuji Shigenobu, Yosuke Tamada, Sato Yoshikatsu, Mitsuyasu Hasebe
    • Journal Title

      Nature Plants

      Volume: 5 Issue: 7 Pages: 681-690

    • DOI

      10.1038/s41477-019-0464-2

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A Novel Katanin-Tethering Machinery Accelerates Cytokinesis2019

    • Author(s)
      Sasaki Takema、Tsutsumi Motosuke、Otomo Kohei、Murata Takashi、Yagi Noriyoshi、Nakamura Masayoshi、Nemoto Tomomi、Hasebe Mitsuyasu、Oda Yoshihisa
    • Journal Title

      Current Biology

      Volume: 29 Issue: 23 Pages: 4060-4070.e3

    • DOI

      10.1016/j.cub.2019.09.049

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Physcomitrella MADS-box genes regulate water supply and sperm movement for fertilization2018

    • Author(s)
      Koshimizu Shizuka、Kofuji Rumiko、Sasaki-Sekimoto Yuko、Kikkawa Masahide、Shimojima Mie、Ohta Hiroyuki、Shigenobu Shuji、Kabeya Yukiko、Hiwatashi Yuji、Tamada Yosuke、Murata Takashi、Hasebe Mitsuyasu
    • Journal Title

      Nature Plants

      Volume: 4 Issue: 1 Pages: 36-45

    • DOI

      10.1038/s41477-017-0082-9

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cytoplasmic MTOCs control spindle orientation for asymmetric cell division in plants2017

    • Author(s)
      Kosetsu Ken、Murata Takashi、Yamada Moe、Nishina Momoko、Boruc Joanna、Hasebe Mitsuyasu、Van Damme Daniel、Goshima Gohta
    • Journal Title

      Proceedings of the National Academy of Sciences

      Volume: 114 Issue: 42

    • DOI

      10.1073/pnas.1713925114

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cells reprogramming to stem cells inhibit the reprogramming of adjacent cells in the moss Physcomitrella patens2017

    • Author(s)
      Sato Yoshikatsu、Sugimoto Nagisa、Hirai Tadayoshi、Imai Akihiro、Kubo Minoru、Hiwatashi Yuji、Nishiyama Tomoaki、Hasebe Mitsuyasu
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 1-12

    • DOI

      10.1038/s41598-017-01786-1

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antheridial development in the moss Physcomitrella patens : implications for understanding stem cells in mosses2017

    • Author(s)
      Kofuji Rumiko、Yagita Yasushi、Murata Takashi、Hasebe Mitsuyasu
    • Journal Title

      Philosophical Transactions of the Royal Society B: Biological Sciences

      Volume: 373 Issue: 1739 Pages: 20160494-20160494

    • DOI

      10.1098/rstb.2016.0494

    • NAID

      120006460358

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Polycomb group protein CLF emerges as a specific tri-methylase of H3K27 regulating gene expression and development in Physcomitrella patens2016

    • Author(s)
      Pereman Idan、Mosquna Assaf、Katz Aviva、Wiedemann Gertrud、Lang Daniel、Decker Eva L.、Tamada Yosuke、Ishikawa Takaaki、Nishiyama Tomoaki、Hasebe Mitsuyasu、Reski Ralf、Ohad Nir
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms

      Volume: 1859 Issue: 7 Pages: 860-870

    • DOI

      10.1016/j.bbagrm.2016.05.004

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] GRAS 転写因子によるヒメツリガネゴケ葉原基の細胞分裂軸制御2020

    • Author(s)
      堀内雄太、藤原彩香、藤本直也、中村亮、吉田彩子、幸節健、壁谷幸子、石川雅樹、玉田洋介、藤本仰一、長谷部光泰、小藤累美子
    • Organizer
      日本植物学会 第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ヒメツリガネゴケの茎葉体における細胞分裂軸制御機構の解析2020

    • Author(s)
      幸節健、堀内雄太、玉田洋介、石川雅樹、壁谷幸子、小藤累美子、長谷部光泰
    • Organizer
      日本植物学会 第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] ABCB14 regulates local cell growth in Physcomitrella patens2020

    • Author(s)
      Zhang, L., Aoyama, T., Murata, T., Kosetsu, K., Kabeya, Y., Ishikawa, M., and Hasebe, M.
    • Organizer
      日本植物学会 第84回大会
    • Related Report
      2020 Annual Research Report
  • [Presentation] Seeking for fundamental role of H3K27me3 and H3.3 in land plants2019

    • Author(s)
      Tamada, Y.
    • Organizer
      The 3rd meeting of the Plant Epigenetics Consortium in Japan, Mishima, Japan
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] STEMIN transcription factor induces stem cell formation with local histone modifications in the moss Physcomitrella patens2019

    • Author(s)
      Ishikawa, M.
    • Organizer
      Marchantia Workshop 2019, Sendai
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 植物の幹細胞新生を司る分子基盤の解明にむけて2019

    • Author(s)
      石川雅樹
    • Organizer
      植物科学シンポジウム2019 SDGsに向けた植物科学の展開
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] STEMIN transcription factor induces reprogramming of differentiated cells to stem cells in the moss Physcomitrella patens.2019

    • Author(s)
      Ishikawa, M., Morishita, M., Kabeya, Y., Hasebe, M.
    • Organizer
      International Symposium: Principles of pluripotent stem cells underlying plant vitality, Sendai
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] STEMIN1 transcription factor induces stem cell formation with local histone modifications in the moss Physcomitrella patens.2019

    • Author(s)
      Morishita, M., Ishikawa, M., Nishiyama, T., Shigenobu, S., Tamada, Y., and Hasebe, M.
    • Organizer
      International Symposium: Principles of pluripotent stem cells underlying plant vitality, Sendai
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Role of histone H3.3 chaperone HIRA in cellular memory of Physcomitrella patens2019

    • Author(s)
      Tamada, Y., Cheng, C., Kabeya, Y., Hasebe, M.
    • Organizer
      Frontiers in plant environmental response research: local signaling, long-distance communication and memory for developmental plasticity, Nagoya, Japan
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Gene regulatory networks on water conductions, flagellate sperm formation, and reprogramming from differentiated cells to stem cells in Physcomitrella patens2018

    • Author(s)
      長谷部光泰
    • Organizer
      EMBO Workshop “New Shores in Land Plant Evolution”, Lisbon, Portugal
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 陸上植物がもつ細胞の分化状態を打破するシステム2018

    • Author(s)
      石川雅樹、森下美生、重信秀治、長谷部光泰
    • Organizer
      第82回日本植物学会大会シンポジウム
    • Related Report
      2018 Annual Research Report
    • Invited
  • [Presentation] ヒメミカヅキモの細胞分裂における微小管再編成過程の可視化2018

    • Author(s)
      村田隆; 川井絢子; 関本弘之; 長谷部光泰
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 分裂期から間期にかけての細胞骨格ダイナミクス2017

    • Author(s)
      村田隆
    • Organizer
      日本植物学会第81回大会シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 核膜-染色体相互作用の植物紡錘体形成における役割2017

    • Author(s)
      村田隆; 大友康平; 日比輝正; 中山博史; 根本知已; 長谷部光泰
    • Organizer
      第69回日本細胞生物学会大会シンポジウム
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] Effects of cellular changes to the evolution of land plant development and life cycle2017

    • Author(s)
      長谷部光泰
    • Organizer
      the Rhynie Chert Discussion Meeting: our earliest terrestrial ecosystem revisited, The Royal Society, London, England
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Generality and Diversity of Development in Land Plants: The Common Ancestor of Land Plants2016

    • Author(s)
      長谷部光泰
    • Organizer
      The 37th New Phytologist Symposium: Plant Developmental Evolution, Beijing, China
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Serial changes of eight types of stem cells in the life cycle of the moss Physcomitrella patens2016

    • Author(s)
      長谷部光泰
    • Organizer
      Session "Life Cycle Evolution", Euro Evo Devo meeting, Uppsala, Sweden
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Generality and Diversity of Development in Land Plants2016

    • Author(s)
      長谷部光泰
    • Organizer
      the annual meeting of Taiwan Society of Plant Biologists, Taichung, Taiwan
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ヒメツリガネゴケの平面内細胞極性における細胞膜局在型タンパク質の機能解析2016

    • Author(s)
      進藤千聖、任俊レン、長谷部光泰、藤田知道
    • Organizer
      日本植物学会 第80回大会、那覇、沖縄県
    • Related Report
      2016 Annual Research Report
  • [Presentation] 紡錘体形成機構のマルチカラー3Dタイムラプス解析2016

    • Author(s)
      村田隆、大友康平、日比輝正、中山博史、根元知己、長谷部光泰
    • Organizer
      日本植物学会 第80回大会、那覇、沖縄県
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒメツリガネゴケのGRAS転写因子SHORT-ROOTによる波層分裂の抑制機構2016

    • Author(s)
      藤原彩花、市川千紘、壁谷幸子、長谷部光泰、小藤累美子
    • Organizer
      日本植物学会 第80回大会、那覇、沖縄県
    • Related Report
      2016 Annual Research Report
  • [Remarks] 研究室web site

    • URL

      http://www.nibb.ac.jp/evodevo

    • Related Report
      2020 Annual Research Report 2019 Annual Research Report
  • [Remarks] 研究室webページ

    • URL

      http://www.nibb.ac.jp/evodevo

    • Related Report
      2018 Annual Research Report 2017 Annual Research Report 2016 Annual Research Report

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Published: 2016-06-01   Modified: 2023-10-16  

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