| Budget Amount *help |
¥189,150,000 (Direct Cost: ¥145,500,000、Indirect Cost: ¥43,650,000)
Fiscal Year 2020: ¥21,190,000 (Direct Cost: ¥16,300,000、Indirect Cost: ¥4,890,000)
Fiscal Year 2019: ¥23,140,000 (Direct Cost: ¥17,800,000、Indirect Cost: ¥5,340,000)
Fiscal Year 2018: ¥20,020,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥4,620,000)
Fiscal Year 2017: ¥44,720,000 (Direct Cost: ¥34,400,000、Indirect Cost: ¥10,320,000)
Fiscal Year 2016: ¥80,080,000 (Direct Cost: ¥61,600,000、Indirect Cost: ¥18,480,000)
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| Outline of Final Research Achievements |
We improved the time-resolution of single-molecule imaging to simultaneously observe several 100s copies of several molecular species even in living cells, accomplishing a 22-μs resolution with a capability of simultaneous super-resolution imaging for every 0.33 s (~50x faster than ever). This technology was applied to the research on three receptors (CD59 for complement regulation, Fcepsilon receptor for allergic reactions, and GPCR-family receptors including opioid receptors). The signaling was found to be enabled by super-transient molecular complexes, consisting of a few to a few 10s of copies of molecules, with lifetimes on the order of 10 s, with a molecular exchange lifetime of ~1 s, and the actin-based membrane skeleton often works as the basis for such molecular complexes.
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