Comprehensive analyses of the interaction between host glycan and influenza viruses and its significance in virus infection

• Project/Area Number: 16H06597
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Veterinary medical science
• Research Institution: National Institute of Advanced Industrial Science and Technology (2017); Hokkaido University (2016)
• Principal Investigator: Hiono Takahiro 国立研究開発法人産業技術総合研究所, 生命工学領域, 研究員 (00775819)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: インフルエンザウイルス / 鳥インフルエンザ / レクチンマイクロアレイ / 糖鎖生物学 / ウイルス学 / シアロ糖 / グライコミクス

Outline of Final Research Achievements

Influenza A virus is a glycan-associated pathogen. In the present study, we analyzed direct interactions of glycan and influenza viruses via HA and NA, as well as host glycome changes after virus infections. The methods for the evaluation of glycan binding the HA and substrate specificity of the NA were established in the present study. Also, the present study demonstrated various changes in host glycome after the influenza virus infection using lectin microarray technique.

Research Products

• [Journal Article] Potency of an inactivated influenza vaccine prepared from A/duck/Hokkaido/162/2013 (H2N1) against a challenge with A/swine/Missouri/2124514/2006 (H2N3) in mice. (2017)
  • Author(s): Suzuki M, Okamatsu M, Hiono T, Matsuno K, Sakoda Y
  • Journal Title: Journal of Veterinary Medical Science Volume: 79 Pages: 1815-1821
  • NAID: 130006199241
  • Peer Reviewed
• [Journal Article] Potency of whole virus particle and split virion vaccines using dissolving microneedle against challenges of H1N1 and H5N1 influenza viruses in mice. (2017)
  • Author(s): (6)Nakatsukasa, A., Kuruma, K., Okamatsu, M., Hiono, T., Suzuki, M., Matsuno, K., Kida, H., Oyamada, T., Sakoda, Y.
  • Journal Title: Vaccine Volume: 35(21) Issue: 21 Pages: 2855-2861
  • DOI: 10.1016/j.vaccine.2017.04.009
  • NAID: 120006463558
  • Peer Reviewed
• [Journal Article] Antigenic diversity of H5 highly pathogenic avian influenza viruses of clade 2.3.4.4 isolated in Asia. (2017)
  • Author(s): (7)Ohkawara, A., Okamatsu, M., Ozawa, M., Chu, D.H., Nguyen, L.T., Hiono, T., Matsuno, K., Kida, H., Sakoda, Y.
  • Journal Title: Microbiology and Immunology Volume: 印刷中 Issue: 5 Pages: 149-158
  • DOI: 10.1111/1348-0421.12478
  • NAID: 120006466464
  • Peer Reviewed
• [Journal Article] Characterization of Highly Pathogenic Avian Influenza Virus A(H5N6), Japan, November 2016. (2017)
  • Author(s): (8)Okamatsu, M., Ozawa, M., Soda, K., Takakuwa, H., Haga, A., Hiono, T., Matsuu, A., Uchida, Y., Iwata, R., Matsuno, K., Kuwahara, M., Yabuta, T., Usui, T., Ito, H., Onuma, M., Sakoda, Y., Saito, T., Otsuki, K., Ito, T., Kida, H.
  • Journal Title: Emerging Infectious Diseases Volume: 23(4) Issue: 4 Pages: 691-695
  • DOI: 10.3201/eid2304.161957
  • NAID: 120006337764
  • Peer Reviewed / Open Access
• [Presentation] 鳥インフルエンザウイルスの糖鎖結合特異性 (2017)
  • Author(s): 日尾野隆大
  • Organizer: 第31回インフルエンザ研究者交流の会シンポジウム
  • Invited