Ultrastructural examination on osteolysis and calcification induced by osteocyte

• Project/Area Number: 16H06602
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Morphological basic dentistry
• Research Institution: Hokkaido University
• Principal Investigator: Hongo Hiromi 北海道大学, 歯学研究院, 学術研究員 (00778970)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 骨細胞性骨溶解 / 骨細胞 / 石灰化沈着

Outline of Final Research Achievements

In this study, we have attempted to histologically verify “osteocytic osteolysis”, using two mice models - RANKL-/- mice lacking osteoclasts and lactating mice fed with calcium (Ca) insufficient diet, which decreased the serum concentration of Ca. Nanoindentation of AFM revealed decreased modulus of elasticity surrounding bone matrix of osteocytes in these model mice. Under TEM observation, there are some fragmented collagen fibrils and pieces of mineralized matrices in the enlarged osteocytic lacunae which showed irregularly-shaped walls. Furthermore, we observed a layer of bone matrix parallel to the configuration of enlarged osteocytic lacunae. Under isotope microscopy using 42Ca stable isotope, the deposition of 42Ca isotope could be observed surrounding the osteocytic lacunae. It seems likely, therefore, that osteocytes enable to erode the surrounding bone matrix and deposit minerals on their lacunae.

Research Products

• [Journal Article] Three-dimensional ultrastructure of osteocytes assessed by focused ion beam-scanning electron microscopy (FIB-SEM) (2018)
  • Author(s): Hasegawa Tomoka、Yamamoto Tomomaya、Hongo Hiromi、Qiu Zixuan、Abe Miki、Kanesaki Takuma、Tanaka Kawori、Endo Takashi、de Freitas Paulo Henrique Luiz、Li Minqi、Amizuka Norio
  • Journal Title: Histochemistry and Cell Biology Volume: 149 Issue: 4 Pages: 423-432
  • DOI: 10.1007/s00418-018-1645-1
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Isotope microscopic assessment for localization of 15N-minodeonate in bone. (2018)
  • Author(s): 2.Hongo H., Sasaki M., Hasegawa T., Tsuboi K., Qiu Z., Amizuka N.
  • Journal Title: Hokkaido Journal of Dental Science. Volume: 38(2) Pages: 123-130
  • NAID: 120006454500
  • Peer Reviewed / Open Access
• [Journal Article] <b>Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical </b><b>bone </b> (2017)
  • Author(s): Sakurai A., Hasegawa T., Kudo A., Shen Z., Nagai T., Abe M., Yoshida T., Hongo H., Yamamoto T., Yamamoto T., Oda K., Freitas de PHL., Li M., Sano H., Amizuka N.
  • Journal Title: Biomedical Research Volume: 38 Issue: 4 Pages: 257-267
  • DOI: 10.2220/biomedres.38.257
  • NAID: 130005966977
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Biological application of focus ion beam-scanning electron microscopy (FIB-SEM) to the imaging of cartilaginous fibrils and osteoblastic cytoplasmic processes. (2017)
  • Author(s): Hasegawa T., Endo T., Tsuchiya E., Kudo A., Zhao S., Moritani Y., Abe M.,Yamamoto T., Hongo H., Tsuboi K., Yoshida T., Nagai T., Khadiza N., Yokoyama A., Freitas de PHL., Li M., Amizuka N.
  • Journal Title: J Oral Biosci. Volume: 59 Issue: 1 Pages: 55-62
  • DOI: 10.1016/j.job.2016.11.004
  • NAID: 120006382296
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Ultrastructural and biochemical aspects of matrix vesicle-mediated mineralization. (2017)
  • Author(s): Hasegawa T., Yamamoto T., Tsuchiya E., Hongo H., Tsuboi K., Kudo A., Abe M., Yoshida T., Nagai T., Khadiza N., Yokoyama A., Oda K., Ozawa H., Freitas de PHL., Li M., Amizuka N.
  • Journal Title: Japanese Dental Science Review Volume: 53巻2号 Issue: 2 Pages: 34-45
  • DOI: 10.1016/j.jdsr.2016.09.002
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Phosphate insufficient diet improves histological abnormalities of alveolar bones in klotho-deficient (kl/kl) mice. (2017)
  • Author(s): Hikone K., Hasegawa T., Hongo H., Sasaki M., Yamamoto T., Oda K., Haraguchi M., Freitas PHL., Li M., Iida J., Amizuka N.
  • Journal Title: Histochem Cytochem. Volume: 印刷中
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] <b>Immunolocalization of osteocyte-derived molecules during bone fracture healing of mouse </b><b>ribs </b> (2016)
  • Author(s): Liu Z., Yamamoto T., Hasegawa T., Hongo H., Tsuboi K., Tsuchiya E., Haraguchi M., Abe M., Freitas PHL., Kudo A., Oda K., Li M., Amizuka N.
  • Journal Title: Biomedical Research Volume: 37 Issue: 2 Pages: 141-151
  • DOI: 10.2220/biomedres.37.141
  • NAID: 130005147430
  • ISSN: 0388-6107, 1880-313X
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Frequency of teriparatide administration affects the histological pattern of bone formation in mice. (2016)
  • Author(s): Yamamoto T., Hasegawa T., Sasaki M., Hongo H., Tsuboi K., Shimizu T., Ohta M., Haraguchi M., Takahata T., Oda K., Freitas PHL., Takakura A., Takao-Kawabata R., Isogai Y., Amizuka N.
  • Journal Title: Endocrinology. Volume: 157(7) Issue: 7 Pages: 2604-2620
  • DOI: 10.1210/en.2015-2028
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Effects of drug discontinuation after short-term daily alendronate administration on osteoblasts and osteocytes. (2016)
  • Author(s): Tsuboi K., Hasegawa T., Yamamoto T., Sasaki M., Hongo H., Oda K., Michigami T., Li M., Freitas PHL., Kitagawa Y., Amizuka N.
  • Journal Title: Histochemistry and Cell Biology Volume: 146巻3号 Issue: 3 Pages: 337-350
  • DOI: 10.1007/s00418-016-1450-7
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Localization of minodronate in mouse femora through isotope microscopy. (2016)
  • Author(s): Hongo, H., Sasaki, M., Kobayashi, S., Hasegawa, T., Yamamoto, T., Tsuboi, K., Tsuchiya, E., Nagai, T., Khadiza, N., Abe, M., Kudo, A., Oda, K., Henrique Luiz de Freitas, P., Li, M., Yurimoto, H. and Amizuka, N.
  • Journal Title: Journal of Histochemistry & Cytochemistry Volume: 64 Issue: 10 Pages: 601-622
  • DOI: 10.1369/0022155416665577
  • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Histological assessment for femora of ovariectomized obesity (db/db) mice carrying mutated leptin receptor. (2016)
  • Author(s): Tanaka Y., Hasegawa T., Yamada T., Yamamoto T., Sasaki M., Hongo H., Tsuboi K., Haraguchi M., de Freitas PHL., Li M., Oda K., Totsuka Y., Tei K., Amizuka N.
  • Journal Title: Histol. Histopathol. Volume: 31(12) Pages: 1315-1326
  • Peer Reviewed / Int'l Joint Research
• [Presentation] アレンドロネートが骨芽細胞系細胞と骨特異性血管に及ぼす組織学的影響． (2018)
  • Author(s): 吉野弘菜、長谷川智香、邱 紫セン、坪井香奈子、本郷裕美、網塚憲生
  • Organizer: 第123回日本解剖学会
• [Presentation] II 型糖尿病モデルSDT fatty ラットにおける下顎骨歯周組織の組織化学的検索． (2016)
  • Author(s): 吉田泰士、本郷裕美、坪井香奈子、長谷川智香、網塚憲生
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌 札幌コンベンションセンター
• [Presentation] オスミウム浸軟法による破骨細胞のゴルジ装置の立体微細構造および分布に関する走査型電子顕微鏡観察． (2016)
  • Author(s): 山本恒之、坪井香奈子、長谷川智香、本郷裕美、網塚憲生
  • Organizer: 第58回歯科基礎医学会学術大会
  • Place of Presentation: 札幌 札幌コンベンションセンター
• [Book] THE BONE (2017)
  • Author(s): 本郷裕美、佐々木宗輝、長谷川智香、坪井香奈子、網塚憲生
  • Total Pages: 5
  • Publisher: メディカルレビュー社 東京