Ultrastructural examination on osteolysis and calcification induced by osteocyte
Project/Area Number |
16H06602
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Morphological basic dentistry
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Research Institution | Hokkaido University |
Principal Investigator |
Hongo Hiromi 北海道大学, 歯学研究院, 学術研究員 (00778970)
|
Project Period (FY) |
2016-08-26 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
|
Keywords | 骨細胞性骨溶解 / 骨細胞 / 石灰化沈着 |
Outline of Final Research Achievements |
In this study, we have attempted to histologically verify “osteocytic osteolysis”, using two mice models - RANKL-/- mice lacking osteoclasts and lactating mice fed with calcium (Ca) insufficient diet, which decreased the serum concentration of Ca. Nanoindentation of AFM revealed decreased modulus of elasticity surrounding bone matrix of osteocytes in these model mice. Under TEM observation, there are some fragmented collagen fibrils and pieces of mineralized matrices in the enlarged osteocytic lacunae which showed irregularly-shaped walls. Furthermore, we observed a layer of bone matrix parallel to the configuration of enlarged osteocytic lacunae. Under isotope microscopy using 42Ca stable isotope, the deposition of 42Ca isotope could be observed surrounding the osteocytic lacunae. It seems likely, therefore, that osteocytes enable to erode the surrounding bone matrix and deposit minerals on their lacunae.
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Report
(3 results)
Research Products
(15 results)
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[Journal Article] <b>Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical </b><b>bone </b>2017
Author(s)
Sakurai A., Hasegawa T., Kudo A., Shen Z., Nagai T., Abe M., Yoshida T., Hongo H., Yamamoto T., Yamamoto T., Oda K., Freitas de PHL., Li M., Sano H., Amizuka N.
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Journal Title
Biomedical Research
Volume: 38
Issue: 4
Pages: 257-267
DOI
NAID
ISSN
0388-6107, 1880-313X
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Biological application of focus ion beam-scanning electron microscopy (FIB-SEM) to the imaging of cartilaginous fibrils and osteoblastic cytoplasmic processes.2017
Author(s)
Hasegawa T., Endo T., Tsuchiya E., Kudo A., Zhao S., Moritani Y., Abe M.,Yamamoto T., Hongo H., Tsuboi K., Yoshida T., Nagai T., Khadiza N., Yokoyama A., Freitas de PHL., Li M., Amizuka N.
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Journal Title
J Oral Biosci.
Volume: 59
Issue: 1
Pages: 55-62
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Ultrastructural and biochemical aspects of matrix vesicle-mediated mineralization.2017
Author(s)
Hasegawa T., Yamamoto T., Tsuchiya E., Hongo H., Tsuboi K., Kudo A., Abe M., Yoshida T., Nagai T., Khadiza N., Yokoyama A., Oda K., Ozawa H., Freitas de PHL., Li M., Amizuka N.
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Journal Title
Japanese Dental Science Review
Volume: 53巻2号
Issue: 2
Pages: 34-45
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Phosphate insufficient diet improves histological abnormalities of alveolar bones in klotho-deficient (kl/kl) mice.2017
Author(s)
Hikone K., Hasegawa T., Hongo H., Sasaki M., Yamamoto T., Oda K., Haraguchi M., Freitas PHL., Li M., Iida J., Amizuka N.
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Journal Title
Histochem Cytochem.
Volume: 印刷中
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] <b>Immunolocalization of osteocyte-derived molecules during bone fracture healing of mouse </b><b>ribs </b>2016
Author(s)
Liu Z., Yamamoto T., Hasegawa T., Hongo H., Tsuboi K., Tsuchiya E., Haraguchi M., Abe M., Freitas PHL., Kudo A., Oda K., Li M., Amizuka N.
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Journal Title
Biomedical Research
Volume: 37
Issue: 2
Pages: 141-151
DOI
NAID
ISSN
0388-6107, 1880-313X
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Frequency of teriparatide administration affects the histological pattern of bone formation in mice.2016
Author(s)
Yamamoto T., Hasegawa T., Sasaki M., Hongo H., Tsuboi K., Shimizu T., Ohta M., Haraguchi M., Takahata T., Oda K., Freitas PHL., Takakura A., Takao-Kawabata R., Isogai Y., Amizuka N.
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Journal Title
Endocrinology.
Volume: 157(7)
Issue: 7
Pages: 2604-2620
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Effects of drug discontinuation after short-term daily alendronate administration on osteoblasts and osteocytes.2016
Author(s)
Tsuboi K., Hasegawa T., Yamamoto T., Sasaki M., Hongo H., Oda K., Michigami T., Li M., Freitas PHL., Kitagawa Y., Amizuka N.
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Journal Title
Histochemistry and Cell Biology
Volume: 146巻3号
Issue: 3
Pages: 337-350
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Localization of minodronate in mouse femora through isotope microscopy.2016
Author(s)
Hongo, H., Sasaki, M., Kobayashi, S., Hasegawa, T., Yamamoto, T., Tsuboi, K., Tsuchiya, E., Nagai, T., Khadiza, N., Abe, M., Kudo, A., Oda, K., Henrique Luiz de Freitas, P., Li, M., Yurimoto, H. and Amizuka, N.
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Journal Title
Journal of Histochemistry & Cytochemistry
Volume: 64
Issue: 10
Pages: 601-622
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Histological assessment for femora of ovariectomized obesity (db/db) mice carrying mutated leptin receptor.2016
Author(s)
Tanaka Y., Hasegawa T., Yamada T., Yamamoto T., Sasaki M., Hongo H., Tsuboi K., Haraguchi M., de Freitas PHL., Li M., Oda K., Totsuka Y., Tei K., Amizuka N.
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Journal Title
Histol. Histopathol.
Volume: 31(12)
Pages: 1315-1326
Related Report
Peer Reviewed / Int'l Joint Research
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