| Project/Area Number |
16H06635
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Molecular biology
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| Research Institution | Tohoku University |
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Principal Investigator |
Iemura Kenji 東北大学, 加齢医学研究所, 助教 (50778058)
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 分裂期 / 染色体動態 / 異数性 / リン酸化 / がん / ゲノム安定性 / 動原体 / 細胞分裂 / 染色体分配 / 染色体不安定性 / 癌 / 細胞・組織 / 染色体 |
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| Outline of Final Research Achievements |
Most of the cancer cells show chromosomal instability (CIN), a condition in which chromosome missegregation occurs at a high rate. Erroneous attachment of kinetochores to microtubules from both spindle poles plays a major role in the generation of CIN. I focused on the phosphorylation of kinetochores that is involved in the correction of erroneous kinetochore-microtubule attachment. In this study, I discovered that cancer cells display reduced chromosome oscillation compared to normal cells, and kinetochore phosphorylation was correlated with chromosome oscillation during metaphase. In addition, I found that chromosome oscillation and kinetochore phosphorylation in metaphase is catalyzed by Aurora A. These data suggest that feedback loop between Aurora A-mediated kinetochore phosphorylation and chromosome oscillation is involved in the maintenance of chromosomal stability through the correction of kinetochore-microtubule attachment.
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