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Development of LC-MS/MS methods to analyze lipid molecules that possess unique structures for the elucidation their functions

Research Project

Project/Area Number 16H06637
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Food science
Research InstitutionYamagata University (2017)
Tohoku University (2016)

Principal Investigator

ITO Junya  山形大学, 農学部, 研究支援者 (50781647)

Project Period (FY) 2016-08-26 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords食品科学 / 過酸化脂質 / 糖化脂質 / 質量分析 / 脂質分析 / 変性脂質 / 修飾脂質
Outline of Final Research Achievements

In this study, we analyzed "lipid molecules that possess unique structures" (i.e., squalene hydroperoxide, glycated lipids and plasmalogen) by LC-MS/MS analysis in the presence of alkali metal ions, since such methods were previously proved to be effective for the analysis of lipid hydroperoxides (e.g., fatty acid hydroperoxide). Since the analysis of these "lipid molecules that possess unique structures" were previously difficult to perform, the developed analytical methods are expected to greatly contribute to the elucidation of further functions and activities of such lipids in foods as well as in animals and humans.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • Research Products

    (16 results)

All 2018 2017 2016 Other

All Journal Article (2 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 1 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 2 results,  Invited: 1 results) Remarks (1 results)

  • [Journal Article] A novel chiral stationary phase LC-MS/MS method to evaluate oxidation mechanisms of edible oils2017

    • Author(s)
      Ito Junya、Shimizu Naoki、Kobayashi Eri、Hanzawa Yasuhiko、Otoki Yurika、Kato Shunji、Hirokawa Takafumi、Kuwahara Shigefumi、Miyazawa Teruo、Nakagawa Kiyotaka
    • Journal Title

      Sci. Rep.

      Volume: 7 Issue: 1 Pages: 10026-10026

    • DOI

      10.1038/s41598-017-10536-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Mass Spectrometric Discrimination of Squalene Monohydroperoxide Isomers2017

    • Author(s)
      Shimizu, N., Bersabe, H., Ito, J., Kato, S., Towada, R., Eitsuka, T., Kuwahara, S., Miyazawa, T., Nakagawa, K.
    • Journal Title

      Journal of Oleo Science

      Volume: 66 Issue: 3 Pages: 227-234

    • DOI

      10.5650/jos.ess16159

    • NAID

      130005397217

    • ISSN
      1345-8957, 1347-3352
    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] トコフェロール酸化物の検出と抗酸化機序の評価:食用油脂への応用2018

    • Author(s)
      丹野玲菜, 加藤俊治, 清水直紀, 伊藤隼哉, 永塚貴弘, 宮澤陽夫, 仲川清隆
    • Organizer
      第29回ビタミンE研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] ヒト皮脂中のスクアレン酸化機構の解析および抗酸化物質によるスクアレン酸化抑制効果の評価2018

    • Author(s)
      清水 直紀, 伊藤 隼哉, 加藤 俊治, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆
    • Organizer
      日本農芸化学会2018年度(平成30年度)大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] エタノールアミン型プラズマローゲンの吸収評価:とくにコリン型への変換について2017

    • Author(s)
      上吉原 怜奈, 乙木 百合香, 伊藤 隼哉, 加藤 俊治, 山下 慎司, 池田 郁男, 宮澤 陽夫, 仲川 清隆
    • Organizer
      日本農芸化学会2017年度(平成29年度)大会
    • Place of Presentation
      京都女子大学(京都府京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] 質量分析を用いた粉乳に含まれる脂質糖化産物の分析法の構築2017

    • Author(s)
      伊藤 隼哉, 小舘 愛, 乙木 百合香, 加藤 俊治, 池田 郁男, 宮澤 陽夫, 仲川 清隆
    • Organizer
      日本農芸化学会2017年度(平成29年度)大会
    • Place of Presentation
      京都女子大学(京都府京都市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Annual Research Report
  • [Presentation] ヒト皮脂中のスクアレンヒドロペルオキシド異性体の分析2017

    • Author(s)
      清水 直紀, 伊藤 隼哉, 加藤 俊治, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆
    • Organizer
      第71回日本栄養・食糧学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] HPLC-MS/MSを用いたリノール酸エステルヒドロペルオキシド異性体の分析法の構築2017

    • Author(s)
      小室 麻里菜, 伊藤 隼哉, 加藤 俊治, 宮澤 陽夫, 仲川 清隆
    • Organizer
      第71回日本栄養・食糧学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 過酸化脂質の異性体解析を通じた皮脂の酸化機構の解明2017

    • Author(s)
      清水 直紀, 伊藤 隼哉, 加藤 俊治, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆
    • Organizer
      第59回日本脂質生化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] LC-MS/MSを用いたヒト皮脂中のスクアレン酸化機構の解析2017

    • Author(s)
      清水 直紀, 伊藤 隼哉, 加藤 俊治, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆
    • Organizer
      日本過酸化脂質・抗酸化物質学会第25回年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] リノール酸過酸化物を指標とした食品の酸化機構の評価2017

    • Author(s)
      伊藤 隼哉、塩見 大樹、加藤 俊治、永塚 貴弘、宮澤 陽夫、仲川清隆
    • Organizer
      第51回日本栄養・食糧学会東北支部大会(50 周年記念)および市民公開講座
    • Related Report
      2017 Annual Research Report
  • [Presentation] Development of analysis method for linoleic acid hydroperoxides using chiral stationary phase LC-MS/MS: Evaluation of lipid oxidation mechanisms2017

    • Author(s)
      Junya Ito, Shunji Kato, Teruo Miyazawa, Kiyotaka Nakagawa
    • Organizer
      The Tohoku Forum for Creativity Thematic Program 2017, "Stage-2, New Horizons in Food Science via Agricultural Immunology"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] LC-MS/MS analysis of squalene hydroperoxide isomers in human skin surface lipids2017

    • Author(s)
      Naoki Shimizu, Junya Ito, Shunji Kato, Takahiro Eitsuka, Teruo Miyazawa, Kiyotaka Nakagawa
    • Organizer
      The Tohoku Forum for Creativity Thematic Program 2017, "Stage-2, New Horizons in Food Science via Agricultural Immunology"
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] リノール酸ヒドロペルオキシドの異性体解析による食品の脂質過酸化機構の評価2017

    • Author(s)
      伊藤 隼哉
    • Organizer
      平成28年度第3回油化学セミナー
    • Place of Presentation
      油脂工業会館(東京都中央区)
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] スクアレンヒドロペルオキシド異性体の高選択的な分析法の構築2016

    • Author(s)
      清水 直紀, Hannah Bersabe, 伊藤 隼哉, 加藤 俊治, 十和田 諒, 桑原 重文, 永塚 貴弘, 宮澤 陽夫, 仲川 清隆
    • Organizer
      第50回日本栄養食糧学会東北支部大会
    • Place of Presentation
      岩手大学農学部(岩手県盛岡市)
    • Related Report
      2016 Annual Research Report
  • [Remarks] 東北大学大学院農学研究科 機能分子解析学分野ホームページ

    • URL

      http://www.agri.tohoku.ac.jp/kinoubun/index-j.html

    • Related Report
      2017 Annual Research Report

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Published: 2016-09-02   Modified: 2019-03-29  

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