| Project/Area Number |
16H06661
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Environmental physiology(including physical medicine and nutritional physiology)
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| Research Institution | University of Tsukuba |
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Principal Investigator |
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | coma / arousal / parabrachial nucleus / lesion / DTA-AAV / IVM-Glu-Cl / pons / DREADD / sleep / locus coereleus / inhibition / activation / pharmocogenetics / electroencephalography |
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| Outline of Final Research Achievements |
Substantial insights into understanding the nature of arousal can be gained by considering the relationship between coma state, sleep and wakefulness. This study is focuses on the fine localization of neuronal regions in the brain and identifying arousal promoting cells and pathways in case of recovery from coma. We first lesion PB nuclei in the pons to produce coma-state mouse. This resulted in ataxia state, followed by coma and death of mice. Inhibition of PB nuclei using IVM-Glu-Cl produced no change to the state. We predicted that noradrenergic LC or dopaminergic vPAG are potential centers for reverse of coma state and chemogenetic activation would produce reverse of coma state. We are currently investigating whether other arousal centers or combined activation may produce arousal from coma in a coma state mouse.
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