Amniotic fluid and serum biomarkers from women with neural tube defect-affected pregnancies

• Project/Area Number: 16H06664
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Neurosurgery
• Research Institution: University of Tsukuba
• Principal Investigator: Tsurubuchi Takao 筑波大学, 医学医療系, 講師 (70778901)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 羊水幹細胞 / 培地 / 培養条件 / 倫理委員会 / 協力病院 / 神経管閉鎖不全症 / 生物学的マーカー / 早期胎内診断 / エピジェネティック機序 / 早期胎内分子診断 / 神経管閉鎖不全 / 脊髄髄膜瘤 / 早期診断 / 基礎的研究

Outline of Final Research Achievements

We aimed to establish the useful biomarkers to detect early in the utero during pregnancy of human neural tube defect. There are many things to be prepared to start clinical studies. Due to the restriction of the special medium in Japan, we have to change the protocol about the medium to grow amniotic fluid stem cells. Amniomax II is useful medium using amniotic fluid stem cells in the world, also in Japan. Our study is on going to establish the novel in the utero therapy using small molecules to decrease the risks of neural tube defect by epigenetic mechanisms.

Research Products

• [Journal Article] 椎弓余剰骨の急速な増大を認めた二分脊椎類縁疾患の一例 (2018)
  • Author(s): 鶴淵隆夫, 室井愛, 稲垣隆介, 山本哲哉, 松村明
  • Journal Title: 小児の脳神経 Volume: 43（１） Pages: 25-29
  • Peer Reviewed
• [Journal Article] 椎弓余剰骨の急速な増大を認めた二分脊椎類縁疾患の一例 (2017)
  • Author(s): 鶴淵 隆夫、稲垣 隆介、室井 愛、坂本 規彰、大谷 明夫、大谷 紀子、山本 哲哉、松村 明
  • Journal Title: 小児の脳神経 Volume: 未定
  • Peer Reviewed
• [Presentation] 脊髄髄膜瘤に伴う脊柱変形に関する臨床学的評価 (2017)
  • Author(s): 2.鶴淵隆夫、室井 愛、津田恭治、稲垣隆介、井原 哲、山本哲哉、松村 明
  • Organizer: 第45回日本小児神経外科学会
• [Presentation] Amniotic fluid and serum biomarkers from women with neural tube defect-affected pregnancies: a case study for myelomeningocele and anencephaly: clinical article (2017)
  • Author(s): Takao Tsurubuchi, Shunsuke Ichi, Kyu-won Shim, William Norkett., Elise Allender, Barbara Mania-Farnell, Tadanori Tomita, David G. McLone, Norman Ginsberg, Shekhar Mayanil, Eiichi Ishikawa, Akira Matsumura
  • Organizer: The 29th KSPN-JSPN joint meeting
  • Int'l Joint Research
• [Presentation] 脊髄髄膜瘤に伴う脊柱変形に関する臨床学的評価 (2017)
  • Author(s): 5.鶴淵隆夫、室井 愛、津田恭治、稲垣隆介、井原 哲、山本哲哉、松村 明
  • Organizer: 第76回日本脳神経外科学会