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Construction of tethered lipid bilayer with domain and its application to membrane protein research

Research Project

Project/Area Number 16H06667
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Physical chemistry
Research InstitutionGunma University

Principal Investigator

MOTEGI TOSHINORI  群馬大学, 大学院理工学府, 助教 (00780602)

Project Period (FY) 2016-08-26 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords脂質二重膜 / 原子間力顕微鏡 / 蛍光顕微鏡 / 膜タンパク質 / 自己組織化単分子膜 / マイカ / 表面・界面物性 / 1分子計測(SMD) / 走査プローブ顕微鏡 / 自己組織化 / ナノバイオ / 平面脂質膜 / 脂質膜ドメイン / 表面修飾 / 生体分子 / 化学物理
Outline of Final Research Achievements

The artificial lipid membrane (SLB) formed on the substrate is useful for understanding the molecular structure of membrane proteins from its two-dimensional nature. On the other hand, structure deformation and lack of orientation due to contact with the substrate are obstacles in membrane protein research. It is necessary to reduce contact with the substrate and immobilize the membrane protein in a specific orientation. In this study, self-assembled monolayer (SAM) binding to a specific site of membrane protein was formed on flat mica, and SLB was formed thereon. It was confirmed that the orientation was aligned from the measurement of the molecular sizes of the membrane proteins with different extracellular domains on the top and bottom. Inactivation of membrane proteins can be reduced and structural analysis of membrane proteins with specific orientation has become possible.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • Research Products

    (16 results)

All 2018 2017 2016 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (12 results) (of which Int'l Joint Research: 3 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Substrate-Induced Structure and Molecular Dynamics in a Lipid Bilayer Membrane2017

    • Author(s)
      Motegi Toshinori、Yamazaki Kenji、Ogino Toshio、Tero Ryugo
    • Journal Title

      Langmuir

      Volume: 33 Issue: 51 Pages: 14748-14755

    • DOI

      10.1021/acs.langmuir.7b03212

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Formation of Cell Membrane Component Domains in Artificial Lipid Bilayer2017

    • Author(s)
      Tero Ryugo、Fukumoto Kohei、Motegi Toshinori、Yoshida Miyu、Niwano Michio、Hirano-Iwata Ayumi
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 17905-17905

    • DOI

      10.1038/s41598-017-18242-9

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Presentation] 表面修飾基板上 の人工脂質膜の物性評価と膜タンパク質再構成系への応用2018

    • Author(s)
      茂木俊憲、星野弘行 、坂本広太 、林 史夫 、園山正史
    • Organizer
      M&BE 応用物理学会 有機分子・バイオエレクトロニクス分科会研究会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Interaction of Protein and Lipid Molecules in the Supported Lipid Bilayer Membrane2018

    • Author(s)
      Toshinori Motegi
    • Organizer
      Gunma International Symposium 2018 on Membranes, 2D Matters and Device Applications
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 表面修飾基板上人工脂質膜の物性評価と膜タンパク質再構成系への応用2018

    • Author(s)
      星野弘行、坂本広太、林 史夫、花屋 実、園山正史、茂木俊憲
    • Organizer
      第65回応用物理学会春季学術講演会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Construction of Artificial Plasma Membrane Model and Elucidation of Functional Structure at Single Molecule Level2018

    • Author(s)
      茂木俊憲
    • Organizer
      NTT物性科学基礎研究所 分子生体機能研究グループ セミナー
    • Related Report
      2017 Annual Research Report
  • [Presentation] Interaction of Protein and Lipid Molecules in the Supported Lipid Bilayer Membrane2018

    • Author(s)
      Toshinori Motegi、Hitoshi Asakawa、Sayaka Matsui、Kazuha Shimizu、Hideki Amii、Toshiyuki Takagi、Toshiyuki Kanamori、Masashi Sonoyama
    • Organizer
      fourth edition of Scanning Probe Microscopy on Soft and Polymeric Materials (SPMonSPM 2018)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Formation of self-assembled monolayer composed of amino terminal phosphonic acid on mica substrate available to supported lipid bilayer system2017

    • Author(s)
      T. Motegi, H. Hoshino, M. Sonoyama
    • Organizer
      第64回応用物理学会春季学術講演会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2017-03-14
    • Related Report
      2016 Annual Research Report
  • [Presentation] 表面修飾した固体基板上人工脂質膜の物性評価と膜タンパク質系への応用2017

    • Author(s)
      星野弘行、坂本広太、林 史夫、花屋 実、園山正史、茂木俊憲
    • Organizer
      平成29年度日本化学会関東支部群馬地区研究交流発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 部分フッ素化脂質膜中蛍光プローブの運動性と膜構造との関連2017

    • Author(s)
      田丸翠允、茂木俊憲、網井秀樹、高木俊之、金森敏幸、園山正史
    • Organizer
      平成29年度日本化学会関東支部群馬地区研究交流発表会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 蛍光プローブの回転運動に基づいた部分フッ素化リン脂質二重膜の局所的流動性の評価2017

    • Author(s)
      田丸翠允、茂木俊憲、園山正史
    • Organizer
      第68回コロイドおよび界面化学討論会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Formation of self-assembled monolayer composed of amino terminal phosphonic acid on mica substrate available to supported lipid bilayer system2017

    • Author(s)
      Toshinori Motegi、Hiroyuki Hoshino、Masashi Sonoyama
    • Organizer
      第54回応用物理学会春季学術講演会 (English Session)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Investigation of fluorescence emission from dye-lipid in partially fluorinated lipid bilayer2016

    • Author(s)
      T. Motegi, R. Tero, T. Takagi, H. Takahashi, H. Amii, T. Kanamori, M. Sonoyama
    • Organizer
      第54回日本生物物理学会大会
    • Place of Presentation
      つくば国際会議場
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
  • [Presentation] アミノ末端を持つホスホン酸からなるマイカ基板上自己組織化単分子膜の形成2016

    • Author(s)
      星野弘行、茂木俊憲 、花屋 実 、白石壮志 、園山正史
    • Organizer
      2016年日本化学会関東支部群馬地区研究交流発表会
    • Place of Presentation
      高崎量子応用研究所-量子科学技術研究開発機構
    • Related Report
      2016 Annual Research Report
  • [Book] 表面修飾基板上の人工脂質膜の物性評価と膜タンパク質再構成系への応用, Molecular Electronics and Bioelectronics (応用物理学会 有機分子・バイオエレクトロニクス分科会誌)2018

    • Author(s)
      茂木俊憲、星野弘行、坂本広太、林 史夫、園山正史
    • Total Pages
      4
    • Publisher
      応用物理学会
    • Related Report
      2017 Annual Research Report
  • [Remarks] 群馬大学 理工学府 分子科学部門 生体分子科学研究室

    • URL

      http://biomolsci.chem-bio.st.gunma-u.ac.jp/~masashi/index.html

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-09-02   Modified: 2019-03-29  

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