Reconstruction of gametic sexual dimorphism on the basis of transcriptomic profile analysis of volvocine algae

• Project/Area Number: 16H06734
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Evolutionary biology
• Research Institution: Kyoto University
• Principal Investigator: HAMAJI TAKASHI 京都大学, 理学研究科, 特定研究員 (10784556)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 異型配偶 / 卵生殖 / 同型配偶 / クラミドモナス / ボルボックス / 進化 / 転写因子 / トランスクリプトーム / 遺伝学 / 緑藻 / 有性生殖 / 雌雄性 / 植物

Outline of Final Research Achievements

Anisogamy emerged in volvocine algae within 200 million years from ancestral isogamous algae. Therefore the comparative omics approaches in volvocine algae should clarify why and how the sexual dimorphism appeared evolutionarily. The present research showed that sex determining regions of anisogamous Eudorina and isogamous Yamagishiella were relatively compact and only two sex-specifically encoded genes: MID in male/minus and FUS1 in female/plus.

Academic Significance and Societal Importance of the Research Achievements

この成果によって、二億年という短い期間で多様化した近縁なボルボックス系列緑藻系統の間で、祖先的な同型配偶から派生的な異型配偶そして卵生殖の生物それぞれの性決定領域の構造が明らかにされたのである。近縁な系統の間での同型配偶・異型配偶・卵生殖の性決定領域の構造の比較は、真核生物の広大な系統の中でも初めてのものであり、ボルボックス系列緑藻で初めて可能となったものであると言える。

Research Products

• [Int'l Joint Research] ウェスタンオンタリオ大学(カナダ)
• [Int'l Joint Research] ドナルド・ダンフォース植物科学センター/アリゾナ大学/カンザス州立大学(米国)
• [Int'l Joint Research] ウェスタンオンタリオ大学(カナダ)
• [Journal Article] Exploring the Limits and Causes of Plastid Genome Expansion in Volvocine Green Algae (2018)
  • Author(s): Gaouda Hager, Hamaji Takashi, Yamamoto Kayoko, Kawai-Toyooka Hiroko, Suzuki Masahiro, Noguchi Hideki, Minakuchi Yohei, Toyoda Atsushi, Fujiyama Asao, Nozaki Hisayoshi, Smith David Roy
  • Journal Title: Genome Biology and Evolution Volume: 10 Issue: 9 Pages: 2248-2254
  • DOI: 10.1093/gbe/evy175
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ゲノム編集の時代における「緑の酵母」クラミドモナスの復権. (2018)
  • Author(s): 浜地貴志, 西村芳樹
  • Journal Title: 生物工学会誌 Volume: 96 Pages: 475-475
  • Open Access
• [Journal Article] Establishment of a clonal culture of unicellular conjugating algae. (2018)
  • Author(s): Tsuchikane Y, Hamaji T, Ota K, Kato S
  • Journal Title: Journal of Visualized Experiments: JoVE Volume: 137 Issue: 137
  • DOI: 10.3791/57761
  • Peer Reviewed / Open Access
• [Journal Article] Anisogamy evolved with a reduced sex-determining region in volvocine green algae (2018)
  • Author(s): Takashi Hamaji, Hiroko Kawai-Toyooka, Haruka Uchimura, Masahiro Suzuki, Hideki Noguchi, Yohei Minakuchi, Atsushi Toyoda, Asao Fujiyama, Shin-ya Miyagishima, James G. Umen, Hisayoshi Nozaki
  • Journal Title: Communications Biology Volume: 1 Issue: 1 Pages: 17-17
  • DOI: 10.1038/s42003-018-0019-5
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Genetic basis for soma is present in undifferentiated volvocine green algae (2017)
  • Author(s): Grochau-Wright ZI, Hanschen ER, Ferris PJ, Hamaji T, Nozaki H, Olson BJSC, Michod RE
  • Journal Title: Journal of Evolutionary Biology Volume: 30 Issue: 6 Pages: 1205-1218
  • DOI: 10.1111/jeb.13100
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Multiple Independent Changes in Mitochondrial Genome Conformation in Chlamydomonadalean Algae. (2017)
  • Author(s): Takashi Hamaji, Hiroko Kawai Toyooka, Atsushi Toyoda, Yohei Minakuchi, Masahiro Suzuki, Asao Fujiyama, Hisayoshi Nozaki and David Roy Smith.
  • Journal Title: Genome Biol Evol. Volume: 9 Issue: 4 Pages: 993-999
  • DOI: 10.1093/gbe/evx060
  • NAID: 120006358595
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Molecular evolutionary analysis of a gender-limited MID ortholog from the homothallic species Volvox africanus with male and monoecious spheroids (2017)
  • Author(s): Kayoko Yamamoto, Hiroko Kawai-Toyooka, Takashi Hamaji, Yuki Tsuchikane, Toshiyuki Mori, Fumio Takahashi, Hiroyuki Sekimoto, Patrick J. Ferris, Hisayoshi Nozaki
  • Journal Title: PLoS One Volume: 12 Issue: 6 Pages: e0180313-e0180313
  • DOI: 10.1371/journal.pone.0180313
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Volvox属Merrillosphaera節における性染色体領域の拡大 (2019)
  • Author(s): 山本荷葉子, 浜地貴志, 豊岡博子, 野口英樹, 水口洋平, 豊田敦, 野崎久義
  • Organizer: 日本藻類学会第43回大会（京都）
• [Presentation] 葉緑体核様体のかたちを司るDNAリガーゼの同定 (2019)
  • Author(s): 西村芳樹，小林優介，浜地貴志，鹿内利治
  • Organizer: 第60回日本植物生理学会年会（名古屋）
• [Presentation] 雌雄異株種・同株種ボルボックスの性染色体領域・相同領域における比較ゲノム解析 (2018)
  • Author(s): 山本荷葉子, 浜地貴志, 豊岡博子, 新垣陽子, 野口英樹, 豊田敦, 水口洋平, 野崎久義
  • Organizer: 日本植物学会大会第82回大会（広島）
• [Presentation] 母性遺伝メカニズムの解明に向けたクラミドモナス有性生殖のゲノム編集アプローチ (2018)
  • Author(s): 浜地貴志, 山岡尚平, 鹿内利治, 西村芳樹
  • Organizer: 日本植物学会大会第82回大会（広島）
• [Presentation] ボルボックス系列異型配偶ユードリナにおける配偶子誘導要因の解析 (2018)
  • Author(s): 豊岡博子, 豊岡博子, 浜地貴志, 西村芳樹, 宮城島進也, 箕浦高子, 野崎久義
  • Organizer: 日本植物学会大会第82回大会（広島）
• [Presentation] 葉緑体核様体ネットワークの動態制御機構 (2018)
  • Author(s): 西村芳樹, 浜地貴志, 小林優介, 鹿内利治
  • Organizer: 日本植物学会大会第82回大会（広島）
• [Presentation] Identification and Characterization of Gamete Adhesion Factor FUS1 Orthologs in Isogamous Yamagishiella and Anisogamous Eudorina (2017)
  • Author(s): Hiroko Kawai-Toyooka, Takashi Hamaji, Haruka Uchimura, Masahiro Suzuki, Hideki Noguchi, Yohei Minakuchi, Atsushi Toyoda, Asao Fujiyama, Shin-ya Miyagishima, Hisayoshi Nozaki
  • Organizer: The Fourth International Volvox Conference
  • Int'l Joint Research
• [Presentation] Anisogamy evolved with the reduced sex-determining region (2017)
  • Author(s): Takashi Hamaji, Hiroko Kawai-Toyooka, Haruka Uchimura, Masahiro Suzuki, Hideki Noguchi, Yohei Minakuchi, Atsushi Toyoda, Asao Fujiyama, Shin-ya Miyagishima, James Umen, and Hisayoshi Nozaki
  • Organizer: The Fourth International Volvox Conference
  • Int'l Joint Research
• [Presentation] Mating type locus-like regions in the homothallic species Volvox africanus (2017)
  • Author(s): Kayoko Yamamoto, Takashi Hamaji, Hiroko Kawai-Toyooka, Hideki Noguchi, Yohei Minakuchi, Atsushi Toyoda, and Hisayoshi Nozaki
  • Organizer: The Fourth International Volvox Conference
  • Int'l Joint Research
• [Presentation] The volvocales genomes project (2017)
  • Author(s): Antariksh Tyagi, Takashi Hamaji, Hiroko Kawai-Toyooka, Masahiro Suzuki, Hideki Noguchi, Yohei Minakuchi, Atsushi Toyoda, Asao Fujiyama, Hisayoshi Nozaki, Bradley J.S.C. Olson
  • Organizer: The Fourth International Volvox Conference
  • Int'l Joint Research
• [Presentation] de novoゲノム配列決定で明らかになったボルボックス科緑藻におけるミトコンドリアDNA形状の進化過程 (2017)
  • Author(s): 浜地 貴志、豊岡 博子、豊田 敦、水口 洋平、鈴木 雅大、藤山 秋佐夫、野崎 久義、SMITH, David Roy
  • Organizer: 日本植物学会第81回大会（野田）
• [Presentation] ボルボックス系列緑藻を用いた異型配偶化の解析：縮小した性決定領域を持つ異型配偶ユードリナ (2017)
  • Author(s): 豊岡 博子、浜地 貴志、他、野崎 久義
  • Organizer: 日本植物学会第81回大会（野田）
• [Presentation] ボルボックス系列緑藻の性決定ゲノム領域と性の進化 (2017)
  • Author(s): 浜地 貴志
  • Organizer: 第二回・性と生殖の懇談会
  • Invited
• [Presentation] Evolution of volvocine mating-type/gender-specific genes deduced from de novo genome sequencing of isogamous Yamagishiella and anisogamous Eudorina (2016)
  • Author(s): Hiroko Kawai-Toyooka, Takashi Hamaji, Haruka Uchimura, Masahiro Suzuki, Atsushi Toyoda, Hideki Noguchi, Yohei Minakuchi, Asao Fujiyama, Shin-ya Miyagishima, Hisayoshi Nozaki
  • Organizer: 17th International Conference on the Cell & Molecular Biology of Chlamydomonas
  • Int'l Joint Research
• [Presentation] Identification and characterization of a cis-regulatory element for zygotic gene expression in Chlamydomonas reinhardtii (2016)
  • Author(s): Takashi Hamaji, David Lopez, Matteo Pellegrini, James Umen
  • Organizer: 17th International Conference on the Cell & Molecular Biology of Chlamydomonas
  • Int'l Joint Research
• [Presentation] ボルボックス系列緑藻のゲノム進化学で追う多細胞性と雌雄性の起源 (2016)
  • Author(s): 浜地 貴志
  • Organizer: 日本進化学会第18回東京大会
  • Invited
• [Presentation] ボルボックス雌雄異体・同体種の進化の分子遺伝学的基盤の解明をめざして (2016)
  • Author(s): 山本荷葉子, 森稔幸, 浜地貴志, 豊岡博子, 野崎久義
  • Organizer: 日本植物学会第80回大会（沖縄）
  • Invited
• [Presentation] ボルボックス系列緑藻における雌雄性獲得前後での性決定領域の比較ゲノム解析 (2016)
  • Author(s): 浜地貴志, 豊岡博子, 内村悠, 鈴木雅大, 豊田敦, 野口英樹, 水口洋平, 藤山秋佐夫, 宮城島進也, 野崎久義
  • Organizer: 日本植物学会第80回大会（沖縄）
• [Remarks] 最初のオスとメスを生み出した性染色体領域を全ゲノム解読から解明
  • URL: http://www.s.u-tokyo.ac.jp/ja/info/5786/
• [Remarks] A small blueprint of “Adam” unveiled
  • URL: https://natureecoevocommunity.nature.com/users/76336-hisayoshi-nozaki/posts/30614-a-small-blueprint-of-adam-unveiled-based-on-de-novo-assemblies-of-four-nuclear-whole-genomes