Analysis of the brain mechanisms responsible for the estrogen-dependent female sexual behavior

• Project/Area Number: 16H06742
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Integrative animal science
• Research Institution: Nippon Medical School (2017); The University of Tokyo (2016)
• Principal Investigator: Watanabe Youki 日本医科大学, 医学部, 助教 (50781788)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: エストロジェン / ロードシス / ChiP-seq / 発情行動 / ERα / VMH / 腹内側核 / 中脳灰白質

Outline of Final Research Achievements

The present study aimed to investigate the brain mechanism mediating the estrogen-dependent female sexual behavior. Previous studies suggested that estrogen control the female-sexual behavior via estrogen-receptor alpha (ERα) in the hypothalamic ventromedial nucleus (VMH). Thus, we focused on the ERα signaling in the VMH in the female rats. We successfully got the gene lists of the ERα binding genes in VMH by genome-wide chromatin Immunoprecipitation sequencing analysis (ChiP-seq). We will clarify the function of these genes in terms of sexual behavior.

Research Products

• [Journal Article] Evaluation of heat stress response in crossbred dairy cows under tropical climate by analysis of heart rate variability (2018)
  • Author(s): Bun, C., Watanabe, Y., Uenoyama, Y., Inoue, N., Ieda, N., Matsuda, F., Tsukamura, H., Kuwahara, M., Maeda, K.-I., Ohkura, S. and Pheng, V.
  • Journal Title: Journal of Veterinary Medical Science Volume: 80 Issue: 1 Pages: 181-185
  • DOI: 10.1292/jvms.17-0368
  • NAID: 130006331058
  • ISSN: 0916-7250, 1347-7439
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Enhancement of the LH surge by male olfactory signals is associated with AVPV Kiss1 cell activation in female rats. (2017)
  • Author(s): Watanabe Y, Ikegami K, Ishigaki R, Ieda N, Uenoyama Y, Maeda KI, Tsukamura H. Inoue N.
  • Journal Title: J Neuroendocrinol Volume: - Issue: 8 Pages: 1-9
  • DOI: 10.1111/jne.12505
  • Peer Reviewed
• [Journal Article] Long -term neonatal estrogen exposure causes irreversible inhibition of LH pulses by suppressing arcuate kisspeptin expression via estrogen receptors α and β in female rodents. (2017)
  • Author(s): Minabe S., Ieda N., Watanabe Y., Inoue, N., Uenoyama Y., Maeda K.-I., and Tsukamura H.
  • Journal Title: Endocrinology Volume: 158(6) Issue: 9 Pages: 1-12
  • DOI: 10.1210/en.2016-1144
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Molecular and Epigenetic Mechanism Regulating Hypothalamic Kiss1 Gene Expression in Mammals. (2016)
  • Author(s): Uenoyama Y, Tomikawa J, Inoue N, Goto T, Minabe S, Ieda N, Nakamura S, Watanabe Y, Ikegami K, Matsuda F, Ohkura S, Maeda KI, Tsukamura H.
  • Journal Title: Neuroendocrinology Volume: 印刷中 Issue: 6 Pages: 640-649
  • DOI: 10.1159/000445207
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Possible Involvement of the Ependymal Cells in the Hindbrain glucose-sensing Mechanism Mediating the Glucoprivic Responses in Male Rats (2018)
  • Author(s): Marimo Sato, Shiori Minabe, Youki Watanabe, Fuko Matsuda and Kei-ichiro Maeda
  • Organizer: ENDO2018
  • Int'l Joint Research
• [Presentation] Augmentation of the LH surge by male olfactory signals is associated with AVPV Kiss1 cell activation in female rats. (2017)
  • Author(s): Watanabe Y, Ishigaki R, Ikegami K, Ieda N, Uenoyama Y, Maeda K-I, Tsukamura H, Inoue N.
  • Organizer: Kisspeptin 2017
  • Place of Presentation: Orland, USA
  • Year and Date: 2017-03-30
  • Int'l Joint Research
• [Presentation] Expression of Gpr101, receptor gene for GnRH metabolite, in KNDy neurons in female rats (2017)
  • Author(s): Nahoko Ieda, Shiori Minabe, Kana Ikegami, Youki Watanabe, Alisa Sugimoto, Naoko Inoue, Yoshihisa Uenoyama, Kei-ichiro Maeda and Hiroko Tsukamura
  • Organizer: 4th World Congress of Reproductive Biology 2017
  • Int'l Joint Research
• [Presentation] Involvement of the ependymal cells in the brain glucose-sensing mechanism to regulate the glucoprivic responses. (2017)
  • Author(s): Marimo Sato, Shiori Minabe, Youki Watanabe, Fuko Matsuda and Kei-ichiro Maeda
  • Organizer: 4th World Congress of Reproductive Biology 2017
  • Int'l Joint Research
• [Presentation] Evaluation of heat stress responses by analysis of heart rate variability in crossbred dairy cows under the tropical climate (2017)
  • Author(s): Chan Bun, Youki Watanabe, Satoshi Ohkura, Yoshihisa Uenoyama, Naoko Inoue, Nahoko Ieda, Fuko Matsuda, Hiroko Tsukamura, Masayoshi Kuwahara, Kei-Ichiro Maeda, and Vutha Pheng
  • Organizer: 4th World Congress of Reproductive Biology 2017
  • Int'l Joint Research
• [Presentation] Amino acid sequences of neuropeptides and their receptors regulating reproduction of sika deer (Cervus nippon) (2017)
  • Author(s): Takahiro Sakono, Shiori Minabe, Youki Watanabe, Fuko Matsuda, Takafumi Ishida and Kei-ichiro Maeda
  • Organizer: 4th World Congress of Reproductive Biology 2017
  • Int'l Joint Research
• [Presentation] 雄由来嗅覚刺激は雌ラット前腹側室周囲核キスペプチンニューロンを 活性化しLH分泌を増強する (2017)
  • Author(s): 渡辺雄貴, 池上花奈, 石垣蓮, 家田菜穂子, 上野山賀久, 前多敬一郎, 束村博子, 井上直子
  • Organizer: 内分泌代謝サマーセミナー
• [Presentation] 後脳上衣細胞のグルコースセンサー細胞としての可能性 (2017)
  • Author(s): 佐藤 真梨萌、美辺 詩織、渡辺 雄貴、松田 二子、前多 敬一郎
  • Organizer: 生殖内分泌学会
• [Presentation] オスラット由来の嗅覚刺激によるメスラット前腹側室周囲核 (AVPV) キスペプチンニューロンの活性化およびLH分泌の増強作用 (2016)
  • Author(s): 渡辺雄貴、井上直子、池上花奈、上野山賀久、束村博子
  • Organizer: 日本繁殖生物学会
  • Place of Presentation: 麻布大学（神奈川県相模原市）
  • Year and Date: 2016-09-11