Research for molecular mechanism of cardiac fibrosis

Research Project

Project/Area Number	16H06748
Research Category	Grant-in-Aid for Research Activity Start-up
Allocation Type	Single-year Grants
Research Field	General internal medicine(including psychosomatic medicine)
Research Institution	The University of Tokyo
Principal Investigator	Koyama Katsuhiro 東京大学, 医学部附属病院, 特任助教 (70780526)
Project Period (FY)	2016-08-26 – 2018-03-31
Project Status	Completed (Fiscal Year 2017)
Budget Amount *help	¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000) Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000) Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords	心臓線維化 / I型コラーゲン / 低酸素誘導因子 / 線維化 / 心筋線維芽細胞
Outline of Final Research Achievements	We have succeeded in establishing cardiac fibroblast cell line from Col1a1-GFP transgenic mice, which express green fluorescent protein (GFP) under the control of Collagen1a1 promoter. Additionally we have found that lactic acid increases Collagen 1a1 transcription in mRNA level in the established cell line. There was an increase in nuclear transcription factor HIF1a protein level in Western blot analysis which suggest Col1a1 transcription level is controlled under HIF1a protein level.

Report

(3 results)