| Project/Area Number |
16H06759
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Otorhinolaryngology
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Akashi Ken 東京大学, 医学部附属病院, 助教 (90779331)
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| Research Collaborator |
YAMASOBA Tatsuya
ANDO Mizuo
YOSHIDA Masafumi
SAITO Yuki
FUKUOKA Osamu
MURAKAMI Yoshinori
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 血中循環腫瘍DNA / デジタルPCR / 頭頸部癌 / EBV関連上咽頭癌 / HPV関連中咽頭癌 / ctDNA / 頭頸部がん / バイオマーカー / ｃｔＤＮＡ / ｃｔDNA / HPV関連中咽頭がん / EBV関連上咽頭がん |
|---|
| Outline of Final Research Achievements |
The objective of this study was to examine whether ctDNA of virus-related head and neck cancer could be detected using droplet digital polymerase chain reaction (PCR), which enables quantification of nucleic acids with a higher accuracy, sensitivity, and precision than traditional real-time PCR.
We collected plasma from blood samples of 4 patients with EBV-related nasopharyngeal cancer and 18 patients with HPV-related oropharyngeal cancer. We extracted cell-free DNA from the plasma and detected ctDNA using droplet digital PCR targeting base sequences of EBV-DNA and HPV-DNA integrated into the DNA of cancer cells. We successfully detected ctDNA in the blood of 3 patients (75%) with EBV-related nasopharyngeal cancer and 10 patients (56%) with HPV-related oropharyngeal cancer.
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