Identification of cell population accelating craniofacial fracture using multiple differentiation method with pluripotent stem cell

• Project/Area Number: 16H06762
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: The University of Tokyo
• Principal Investigator: Kanke Kosuke 東京大学, 医学部附属病院, 助教 (90779810)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 多能性幹細胞 / 骨芽細胞 / 顎顔面骨骨折

Outline of Final Research Achievements

This study designed　which　cell　population　contributes　to　bone　healing　in　craniofacial　fracture　with　ositeobleast induction using plurioptent stem cell. During induction, genes of Dermo1, Runx2, Sp7 and Bglap highly expressed at day 9, day Day 19 and Day 23, respectively. Genes of Runx2 ann Sp7 were expressed at the same levels of these of primary osteoblasts. 2.3kb Col1a1 was highly expressed as well. We are researching how these populations contirbute to craniofacial bone fracture healing.

Research Products

• [Journal Article] Three-dimensional system enabling the maintenance and directed differentiation of pluripotent stem cells under defined conditions (2017)
  • Author(s): Zujur D, Kanke K, Lichtler AC, Hojo H, Chung UI, Ohba S
  • Journal Title: Science Advanvces Volume: 3 Issue: 5
  • DOI: 10.1126/sciadv.1602875
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] In-hospital surgical treatment for haemorrhage after aesthetic mandibular osteotomy performed as an office-based day surgery: A case report. (2017)
  • Author(s): Kanke K, Abe T, Abe M, Mori Y, Hoshi K and Takato T.
  • Journal Title: Annals of Medicine and Surgery Volume: 24 Pages: 15-18
  • DOI: 10.1016/j.amsu.2017.10.001
  • Peer Reviewed / Open Access
• [Presentation] 頬部膨隆を呈した乳児期の歯原性粘液線維腫の一例 (2017)
  • Author(s): 菅家康介
  • Organizer: 第62回公益社団法人日本口腔外科学会総会・学術大会
• [Presentation] 全前脳胞症を伴う正中上唇裂における当科の周術期管理 (2017)
  • Author(s): 菅家康介
  • Organizer: 第29回一般社団法人日本小児口腔外科学会総会・学術大会
• [Presentation] Differentiation of hiPSCs into Osteoblasts by Using Small Molecule Inducers Under Fully Defined Xeno-free Conditions. (2016)
  • Author(s): Zujur DC, Kanke K, Chung UI, Ohba S
  • Organizer: ASBMR2016
  • Place of Presentation: Atlanta, USA, Georgia World Congress Center
  • Year and Date: 2016-09-16
  • Int'l Joint Research
• [Presentation] Three Dimensional Differentiation of Mouse Pluripotent Stem Cells into Osteogenic Cells under Defined Conditions. (2016)
  • Author(s): Zujur DC, Kanke K, Chung UI, Ohba S
  • Organizer: ASBMR2016
  • Place of Presentation: Atlanta, USA, Georgia World Congress Center
  • Year and Date: 2016-09-16
  • Int'l Joint Research
• [Presentation] サイナスリフト後の上顎臼歯部広範囲欠損症例に対しメッシュプレートを用いて骨増生術を施行した1例 (2016)
  • Author(s): 内山貴夫, 西條英人, 杉山円, 菅野勇樹, 菅家康介, 髙戸毅
  • Organizer: 第19回日本先進インプラント医療学会学術大会・総会
  • Place of Presentation: 一ツ橋記念講堂, 東京
  • Year and Date: 2016-09-04
• [Remarks] 東京大学医学部附属病院口腔顎顔面外科・矯正歯科のホームページ
  • URL: https://plaza.umin.ac.jp/~oralsurg/