Identification and validation of the angiogenic adipose-derived stem cells among patients with cardiovasciular diseases
| Project/Area Number |
16H06828
|
|---|
| Research Category |
Grant-in-Aid for Research Activity Start-up
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Cardiovascular medicine
|
|---|
| Research Institution | Kanazawa University |
|---|
Principal Investigator |
Oto Inoue 金沢大学, 附属病院, 医員 (00781147)
|
|---|
| Project Period (FY) |
2016-08-26 – 2018-03-31
|
| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
|---|
| Keywords | 脂肪組織由来間葉系幹細胞 / 血管新生 / インスリン抵抗性 / 脂肪組織慢性炎症 / 再生医学 |
|---|
| Outline of Final Research Achievements |
Adipose-derived stem cells (ADSCs) have been emerged as an attractive option for regenerative therapy. ADSCs are composed of various subsets and the CD271+ subset is reported to be more primitive and proliferative. ADSCs were isolated from subcutaneous adipose tissue of 20 patients undergoing elective cardiac surgery. The percentages of CD271+ subset in CD45-CD34+ ADSCs were measured by flowcytometer. According to the CD271 frequency, ADSCs samples were divided into two groups (“high” and “low” groups). Nude mice were subjected to hindlimb ischemia and 1 million of ADSCs) were injected into the ischemic muscles. At day 14, neovascularization was evaluated by laser Doppler analysis and histological analysis using fluorescent-labeled lectin perfusion in duplicate. Intramuscular injection of ADSCs of the “high” group demonstrated enhanced neovascularization compared to the “low” group in laser Doppler analysis and also in histological analysis.
|
Report
(3 results)
Research Products
(8 results)
