| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Outline of Final Research Achievements |
Adipose-derived stem cells (ADSCs) have been emerged as an attractive option for regenerative therapy. ADSCs are composed of various subsets and the CD271+ subset is reported to be more primitive and proliferative. ADSCs were isolated from subcutaneous adipose tissue of 20 patients undergoing elective cardiac surgery. The percentages of CD271+ subset in CD45-CD34+ ADSCs were measured by flowcytometer. According to the CD271 frequency, ADSCs samples were divided into two groups (“high” and “low” groups). Nude mice were subjected to hindlimb ischemia and 1 million of ADSCs) were injected into the ischemic muscles. At day 14, neovascularization was evaluated by laser Doppler analysis and histological analysis using fluorescent-labeled lectin perfusion in duplicate. Intramuscular injection of ADSCs of the “high” group demonstrated enhanced neovascularization compared to the “low” group in laser Doppler analysis and also in histological analysis.
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