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Structural transition of de novo proteins and reconstruction of protein nanobuilding block complexes.

Research Project

Project/Area Number 16H06837
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Bio-related chemistry
Research InstitutionInstitute for Molecular Science (2017)
Shinshu University (2016)

Principal Investigator

KOBAYASHI Naoya  分子科学研究所, 協奏分子システム研究センター, 特任研究員 (60781945)

Project Period (FY) 2016-08-26 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords人工蛋白質 / 蛋白質ナノブロック / 自己組織化 / 環境変化応答 / ダイナミクス解析 / 環境応答
Outline of Final Research Achievements

The purposes of this research are to design environmental change responsive artificial proteins which dynamically changes the structure between monomer and dimer, to investigate association state of the proteins in response to environmental change, and to analyze the dynamics of self-assembled nanostructure formation by applying these environmental change responsive artificial proteins as protein nanobuilding blocks.
In particular, we aim to create and control protein nanostructures responding to environmental changes by development and analysis of artificial proteins and protein nanoblocks responsive to pH changes.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • Research Products

    (15 results)

All 2018 2017 2016

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 2 results,  Open Access: 4 results) Presentation (10 results) (of which Int'l Joint Research: 4 results,  Invited: 5 results)

  • [Journal Article] Self-Assembling Supramolecular Nanostructures Constructed from de Novo Extender Protein Nanobuilding Blocks2018

    • Author(s)
      Kobayashi Naoya、Inano Kouichi、Sasahara Kenji、Sato Takaaki、Miyazawa Keisuke、Fukuma Takeshi、Hecht Michael H、Song Chihong、Murata Kazuyoshi、Arai Ryoichi
    • Journal Title

      ACS Synthetic Biology

      Volume: 7 Issue: 5 Pages: 1381-1394

    • DOI

      10.1021/acssynbio.8b00007

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Design and construction of self-assembling supramolecular protein complexes using artificial and fusion proteins as nanoscale building blocks2017

    • Author(s)
      Naoya Kobayashi, Ryoichi Arai
    • Journal Title

      Current Opinion in Biotechnology

      Volume: 46 Pages: 57-65

    • DOI

      10.1016/j.copbio.2017.01.001

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 二量体形成新規人工タンパク質を用いたタンパク質ナノブロック開発による自己組織化ナノ構造複合体の創製2016

    • Author(s)
      小林直也,新井亮一
    • Journal Title

      分子研レターズ

      Volume: 73 Pages: 30-31

    • Related Report
      2016 Annual Research Report
    • Open Access
  • [Journal Article] 人工蛋白質で「かたち」をつくろう―ブロック遊びしようよ!―2016

    • Author(s)
      小林直也,新井亮一
    • Journal Title

      生物工学会誌

      Volume: 94 Pages: 270-270

    • Related Report
      2016 Annual Research Report
    • Open Access
  • [Journal Article] バイナリーパターン配列デザインによるデノボ蛋白質の創出と蛋白質ナノブロックによる超分子複合体の創生2016

    • Author(s)
      小林直也,木村尚弥,新井亮一
    • Journal Title

      生物工学会誌

      Volume: 94 Pages: 485-488

    • Related Report
      2016 Annual Research Report
    • Open Access
  • [Presentation] pH-dependent structural transition of self-assembling nanostructures constructed from protein nanobuilding blocks using a dimeric de novo protein2017

    • Author(s)
      Naoya Kobayashi, Takuya Sengoku, Takaaki Sato, Ryoichi Arai
    • Organizer
      Okazaki Conference 2017 on Grand Challenges in Small-angle Scattering
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市)
    • Year and Date
      2017-03-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Self-assembling supramolecular nanostructures created from de novo protein nanobuilding blocks2017

    • Author(s)
      Naoya Kimura, Naoya Kobayashi, Ryoichi Arai
    • Organizer
      Okazaki Conference 2017 on Grand Challenges in Small-angle Scattering, Okazaki
    • Place of Presentation
      岡崎コンファレンスセンター(愛知県岡崎市)
    • Year and Date
      2017-03-18
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] タンパク質ナノブロックによる動的秩序構造形成2017

    • Author(s)
      木村尚弥、小林直也、新井亮一
    • Organizer
      日本化学会第97春季年会
    • Place of Presentation
      慶應義塾大学日吉キャンパス(神奈川県横浜市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] Dynamical Ordering Nanostructures Constructed from Protein Nanobuilding Blocks2017

    • Author(s)
      Naoya Kimura, Naoya Kobayashi, Ryoichi Arai
    • Organizer
      The 5th International Symposium of Dynamical Ordering of Biomolecular Systems for Creation of Integrated Functions
    • Place of Presentation
      東京大学(東京都目黒区)
    • Year and Date
      2017-01-21
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] pH-dependent structural transition of a dimeric de novo protein WA20 and reconstruction of protein nanobuilding block complexes2017

    • Author(s)
      Naoya Kobayashi, Takuya Sengoku, Takaaki Sato, Ryoichi Arai
    • Organizer
      第17回日本蛋白質科学会年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 人工蛋白質ナノブロックによる多様な自己組織化超分子ナノ構造複合体:ネオバイオ超分子の創生を目指して2016

    • Author(s)
      小林直也、木村尚弥、新井亮一
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-11-30
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] 人工タンパク質をブロックに見立てた超分子ナノ構造複合体の設計構築2016

    • Author(s)
      小林直也、木村尚弥、新井亮一
    • Organizer
      第54回日本生物物理学会年会
    • Place of Presentation
      つくば国際会議場(茨城県つくば市)
    • Year and Date
      2016-11-25
    • Related Report
      2016 Annual Research Report
    • Invited
  • [Presentation] De Novo Protein Nano-Building Block Approach for “Synthetic Structural Biology” to Create Artificial Supramolecular Protein Complexes2016

    • Author(s)
      Ryoichi Arai, Naoya Kimura, Naoya Kobayashi, Michael H. Hecht
    • Organizer
      THE 42nd NAITO CONFERENCE ON In the Vanguard of Structural Biology: Revolutionizing Life Sciences
    • Place of Presentation
      シャトレーゼ ガトーキングダム サッポロ(北海道札幌市)
    • Year and Date
      2016-10-04
    • Related Report
      2016 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 蛋白質ナノブロック用超安定化人工蛋白質SUWA (Super WA20)の特性解析及び構造解析2016

    • Author(s)
      木村尚弥、小林直也、新井亮一
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場・ANAクラウンプラザホテル(富山県富山市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
  • [Presentation] Self-assembling supramolecular nano-architectures created from de novo protein nano-building blocks2016

    • Author(s)
      Naoya Kimura, Naoya Kobayashi, Ryoichi Arai
    • Organizer
      第68回日本生物工学会大会
    • Place of Presentation
      富山国際会議場・ANAクラウンプラザホテル(富山県富山市)
    • Year and Date
      2016-09-28
    • Related Report
      2016 Annual Research Report
    • Invited

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Published: 2016-09-02   Modified: 2019-03-29  

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