| Project/Area Number |
16H06843
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Gifu University |
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Principal Investigator |
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | プロキラルジオール / 不斉酸化 / ヒドロキシアルカン酸 / アルコール脱水素酵素 / プロテオーム解析 / 微生物スクリーニング / 不斉酸化酵素 / 非天然アミノ酸 |
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| Outline of Final Research Achievements |
In the first year, we found microbes A and B asymmetrically oxidizing 2,2-diethyl-1,3-propanediol. As a result of optical purity analysis, strain A synthesized (R)-hydroxypropanoate at 65%ee, and strain B synthesized (S)-hydroxypropanoate at 33%ee.
In the second year, we aimed to isolate and characterize the genes coding asymmetrically oxidizing enzyme using heterologous expression system. Several candidate genes were selected through both proteome and whole-genome analysis, and were heterologously expressed. As a result of optical purity analysis, three recombinants exhibited (R)-selectivity. One of the recombinant expressing an alcohol dehydrogenase synthesized (R)-hydroxypropanoate at 90%ee.
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