Functional analysis of the novel apoptosis suppressor Apsup from the baculovirus Lymantria dispar multiple nucleopolyhedrovirus
Project/Area Number |
16H06866
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Insect science
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Research Institution | Nagoya University |
Principal Investigator |
Yamada Hayato 名古屋大学, 生命農学研究科, 助教 (70778258)
|
Project Period (FY) |
2016-08-26 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | バキュロウイルス / Apsup / アポトーシス / マイマイガ / 抗ウイルス応答 / 昆虫培養細胞 / 核多角体病ウイルス / APSUP / 昆虫 / 昆虫科学 / ウイルス学 |
Outline of Final Research Achievements |
We have previously identified a novel apoptosis suppressor Apsup from the baculovirus Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV). Apsup suppresses apoptosis by preventing proteolytic processing of L. dispar initiator caspase Ld-Dronc. Here, we performed mutation analyses of Apsup to determine functional domains in Apsup. Mutation analyses indicated that the N-terminal region in Apsup required for interacting with Ld-Dronc, and the C-terminal region in Apsup required for preventing the proteolytic processing of Ld-Dronc. Our findings provide clues to understand the mechanisms by which Apsup suppresses apoptosis.
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Report
(3 results)
Research Products
(14 results)