The hepatosomes released from damaged hepatocytes will contribute the progression of ALD

• Project/Area Number: 16H06872
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Gastroenterology
• Research Institution: Mie University
• Principal Investigator: Eguchi Akiko 三重大学, 医学系研究科, 特任助教(研究担当) (00598980)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 肝臓 / 細胞外小胞 / 消化器 / 細胞間伝達 / 細胞・組織

Outline of Final Research Achievements

We revealed that damaged hepatocytes treated with alcohol released extracellular vesicles (EVs) and these EVs were contained the cellular composition from damaged hepatocytes using alcoholic liver disease mouse model. To investigate EV biological function, we isolated hepatic macrophages and incubated hapatic macrophages with EVs. EVs were internalized in the hepatic macrophages resulted in activation of hepatic macrophages. These resulted indicated that hepatocyte-derived EVs contributed the progression of alcoholic liver disease as cell-to-cell communicators. We will identify the EV composition to associate with hepatic macrophage activation and will develop new therapeutic approaches for inhibition of hepatic macrophage activation in future study.

Research Products

• [Presentation] Hepatocyte-derived EVs (extracecullar vesicles) in alcoholic steatohepatitis contain specific microRNA barcode and modulate macrophage phenotype (2017)
  • Author(s): Akiko Eguchi, Yoshiyuki Takei, Hidekazu Tsukamoto and Ariel E. Feldstein
  • Organizer: Asia-Pacific Society for Alcohol and Addiction Research
  • Int'l Joint Research