Screening of microorganisms metabolizing lipids aerobically under inhibition of be-ta oxidation and construction of oxidized lipid library
| Project/Area Number |
16H06893
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Applied microbiology
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Research Collaborator |
Kishino Shigenobu 京都大学, 大学院農学研究科, 助教 (40432348)
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 酸化脂質 / 水酸化脂肪酸 / 共役脂肪酸 / 応用微生物 / 脂質 / 微生物 / 機能性脂質 / リポキシゲナーゼ / P450 |
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| Outline of Final Research Achievements |
Oxidative lipid metabolites are expected to have physiological functions. Expansion of lipid library was tried by two methods, 'conversion of original fatty acids by lipoxygenase and P450' and 'screening of microorganisms converting fatty acid oxidatively under inhibition of beta-oxidation. As a result of screening, the microorganism producing novel hydroxy fatty acid was obtained. In addition, it was revealed that hydroxy and oxo fatty acids from lactic acid bacteria were converted to novel epoxy fatty acids by P450 BM-3.
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Report
(3 results)
Research Products
(3 results)
