Elucidation of the mechanism in organ-specific reguation of thyroid hormone deiodinase

• Project/Area Number: 16H06902
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Endocrinology
• Research Institution: Kyoto University
• Principal Investigator: Sakane Yoriko 京都大学, 医学研究科, 特定助教 (70781342)
• Research Collaborator: YAMAUCHI Ichiro
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 甲状腺ホルモン脱ヨード酵素 / 甲状腺ホルモン / 化合物ライブラリー / 甲状腺脱ヨード酵素 / 甲状腺 / 臓器特異的

Outline of Final Research Achievements

Based on our progress last year, we published two articles. In one article, we demonstrated that LXRα which was expressed mainly in the liver regulated the transcription of thyroid hormone deiodinase type 1 (D1) and also demonstrated the crosstalk between LXRα and thyroid hormone receptor on the promoter region of human D1 gene. In the other article, we demonstrated that growth hormon regulated the concentration of thyroid hormone levels and only in thyroid cell lines, growth hormon increased the activity of thyroid hormone deiodinase type 2.
In addition, to investigate novel regulators of the activity of thyroid hormone deiodinases we started to establish new promoter assay system using chemical library.

Research Products

• [Journal Article] Diverse metabolic effects of O-GlcNAcylation in the pancreas but limited effects in insulin-sensitive organs in mice. (2017)
  • Author(s): Ida S, Morino K, Sekine O, Ohashi N, Kume S, Chano T, Iwasaki K, Harada N, Inagaki N, Ugi S, Maegawa H.
  • Journal Title: Diabetologia Volume: 60 (9) Issue: 6 Pages: 1761-1769
  • DOI: 10.1371/journal.pone.0179213
  • Peer Reviewed / Open Access
• [Journal Article] Effects of growth hormone on thyroid function are mediated by type 2 iodothyronine deiodinase in humans (2017)
  • Author(s): Yamauchi Ichiro、Sakane Yoriko、Yamashita Takafumi、Hirota Keisho、Ueda Yohei、Kanai Yugo、Yamashita Yui、Kondo Eri、Fujii Toshihito、Taura Daisuke、Sone Masakatsu、Yasoda Akihiro、Inagaki Nobuya
  • Journal Title: Endocrine Volume: 59 Issue: 2 Pages: 353-363
  • DOI: 10.1007/s12020-017-1495-y
  • Peer Reviewed / Open Access