Establishment of cell culture system for drug development using organ derived decellularized tissue
Project/Area Number |
16H06903
|
Research Category |
Grant-in-Aid for Research Activity Start-up
|
Allocation Type | Single-year Grants |
Research Field |
Digestive surgery
|
Research Institution | Kyoto University |
Principal Investigator |
Fukumitsu Ken 京都大学, 医学研究科, 特定病院助教 (70700516)
|
Project Period (FY) |
2016-08-26 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
|
Keywords | 再生医療 / 人工臓器 / 創薬 / 組織工学 |
Outline of Final Research Achievements |
hepatocellular carcinoma cell line was engrafted in decellularized liver scaffold and characterized as cancer cell. As a result, it was found that the signl of epithelial-mesenchymal-transition was activated in decellularized scaffold derived from fibrous liver compared with that derived from normal liver. Adding to this, the FAK signal was activated as a mechanism. In other words, it was shown that the extracellular matrix in fibrotic liver may provide an environment susceptible to epithelial mesenchymal transition to cancer cells. From the above, it was shown that the hardness and constituent elements of the extracellular matrix affect the malignancy and metastatic potential of the cancer cells.
|
Report
(3 results)
Research Products
(30 results)
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] A novel culture system preserving natural scaffolds of fibrotic livers accelerates progression of hepatocellular carcinoma cells2018
Author(s)
Yuya Miyauchi, Kentaro Yasuchika Yu Oshima, Hiroshi Kawamoto, Takahito Minami, Hidenobu Kojima, Ryoya Yamaoka, Takayuki Kawai, Satoshi Ogiso, Ken Fukumitsu, Takamichi Ishii, Shinji Uemoto
Organizer
The Asian Pacific Association for the Study of the Liver Single topic Conference on HCC
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-
[Presentation] A novel three-dimensional culture system, decellularized-tissue obtained from cirrhotic livers, enhances an epithelial-mesenchymal transition phenotype in HCC cells2017
Author(s)
Yuya Miyauchi, Kentaro Yasuchika, Ken Fukumitsu, Satoshi Ogiso, Hidenobu Kojima, Ryoya Yamaoka, Takayuki Kawai, Hokahiro Katayama, Elena Y Yoshitoshi, Sadahiko Kita, Katsutaro Yasuda, Naoya Sasaki, Takamichi Ishii, Junji Komori, Shinji Uemoto
Organizer
American college of Surgeons clinical congress
Related Report
Int'l Joint Research
-
-
-
-
-
-
-
-