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Analysis of the translocation mechanism of CKAP4 to exosome

Research Project

Project/Area Number 16H06944
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionOsaka University

Principal Investigator

Kimura Hirokazu  大阪大学, 医学系研究科, 特任助教(常勤) (60595370)

Project Period (FY) 2016-08-26 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
KeywordsCKAP4 / エクソソーム / DKK1 / クラスリン / エンドサイトーシス / Clathrin / がん / Dkk1
Outline of Final Research Achievements

In this study, translocation mechanism of CKAP4, a novel receptor of secreted protein DKK1, to exosome was analyzed. CKAP4 was specifically secreted with exosome from cancer cell lines. In addition, the amounts of CKAP4 on exosome were proportional to those of CKAP4 on the cell surface membrane. The secretion of CKAP4- containing exosome was mediated by DKK1- and Clathrin- dependent endocytosis rotes. These results suggest that CKAP4, which functions as a receptor of DKK1 on the cell surface membrane of cancer cells, is translocated to exosome, and CKAP4 in exosome may be a novel tumor marker.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • Research Products

    (5 results)

All 2018 2017 2016

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results)

  • [Journal Article] Activation of the Dickkopf1-CKAP4 pathway is associated with poor prognosis of esophageal cancer and anti-CKAP4 antibody may be a new therapeutic drug.2018

    • Author(s)
      Shinno N, Kimura H, Sada R, Takiguchi S, Mori M, Fumoto K, Doki Y, Kikuchi A.
    • Journal Title

      Oncogene

      Volume: 未確定 Issue: 26 Pages: 602-612

    • DOI

      10.1038/s41388-018-0179-2

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Dickkopf1-cytoskeleton-associated protein 4 axis creates a novel signalling pathway and may represent a molecular target for cancer therapy.2017

    • Author(s)
      Kikuchi A, Fumoto K, Kimura H.
    • Journal Title

      Br J Pharmacol

      Volume: 174(24) Issue: 24 Pages: 4651-4665

    • DOI

      10.1111/bph.13863

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Presentation] 食道扁平上皮癌の新規治療標的であるDKK1-CKAP4シグナル2017

    • Author(s)
      木村 公一
    • Organizer
      第76回癌学会学術総会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 膵癌の新規治療標的であるDkk1新規受容体CKAP42017

    • Author(s)
      木村 公一
    • Organizer
      第48回日本膵臓学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Dkk1-CKAP4 signaling pathway represents a novel therapeutic2016

    • Author(s)
      Hirokau Kimura
    • Organizer
      日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜
    • Year and Date
      2016-10-06
    • Related Report
      2016 Annual Research Report

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Published: 2016-09-02   Modified: 2019-03-29  

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