The role of Heme Oxygenase-1 in neonatal sepsis using preterm mouse model
Project/Area Number |
16H06971
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Embryonic/Neonatal medicine
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Research Institution | Kobe University |
Principal Investigator |
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Project Period (FY) |
2016-08-26 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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Keywords | neonatal sepsis / Heme oxygenase-1 / 早産児マウス敗血症モデル / mouse model / preterm sepsis / cytokine / immunity / inflammation / 新生児 / 早産児敗血症 / 動物モデル / Heme Oxygenase / 糞便懸濁液 / ノックアウトマウス / 非外科的敗血症モデル / ヘムオキシゲナーゼ / 感染症 / トランスレーショナルリサーチ / 敗血症 / 早産児 / マウスモデル / HO-1 / 新生児敗血症 / cecal slurry / 敗血症モデル / ヘムオキシゲナーゼ-1 / 敗血症動物モデル / Cecal slurry / サイトカイン |
Outline of Final Research Achievements |
To induce sepsis, the cecal slurry (CS) model was applied to 4d-old mouse pups. In brief, adult cecums were harvested, and their contents were diluted with PBS-glycerol for CS stock preparations. HO-1 heterozygote (Het, HO-1+/-) and wild-type (WT) mice were administered CS IP at a dose of 2.0-mg/g (LD40 for WT mice, Fujioka et al, Shock 47:242, 2017), and then monitored for survival. To study the protective role of HO-1 induction, 30-micro mol heme/kg was given to 3d-old pups of both genotypes 24h prior to sepsis induction. Treatment with 2.0-mg CS/g caused a significantly higher mortality in Het (85.0%) than WT (40.9%, p<0.01) pups. Most importantly, induction of HO-1 by heme significantly reduced mortality in both WT (6.3%) and Het (23.5%) pups. In addition, expression profiles measured using PCR arrays showed significant increases in cytokines in both genotypes 6h post-sepsis induction. This increase was generally attenuated in pups of both genotypes pre-treated with heme.
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Report
(3 results)
Research Products
(61 results)
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[Journal Article] A genome-wide association study identifies only two ancestry specific variants associated with spontaneous preterm birth.2018
Author(s)
Rappoport N, Toung J, Hadley D, Wong RJ, Fujioka K, Jelliffe-Pawlowski LL, Gould JB, Darmstadt GL, Wang X, Bustamante CD, Snyder MP, Ziv E, Patsopoulos NA, Muglia LJ, Burchard E, Shaw GM, O'Brodovich HM, Stevenson DK, Butte AJ, Sirota M, et al.
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Journal Title
Sci Rep.
Volume: 8(1)
Issue: 1
Pages: 226-226
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Nontypeable Haemophilus Influenzae Sepsis in a Term Neonate2018
Author(s)
SAKI OKUBO, KAZUMICHI FUJIOKA, MEGUMI YAMANE, MARIKO ASHINA, SACHIYO FUKUSHIMA, TOSHIHIKO IKUTA, SHOHEI OHYAMA, KEIJI YAMANA, AKIKO KOBAYASHI, KAZUMOTO IIJIMA, ICHIRO MORIOKA
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Journal Title
Kobe Journal of Medical Sciences
Volume: 4
Pages: 105-108
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] A Case of Congenital Complete Atrioventricular Block Treated with Transdermal Tulobuterol2018
Author(s)
TOSHIHIKO IKUTA, KAZUMICHI FUJIOKA, YUMI SATO, MARIKO ASHINA, SACHIYO FUKUSHIMA, SHOHEI OHYAMA, SAKI OKUBO, KEIJI YAMANA, MAYUMI MORIZANE, KENJI TANIMURA, MASASHI DEGUCHI, KAZUMOTO IIJIMA, ICHIRO MORIOKA, HIDETO YAMADA
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Journal Title
Kobe Journal of Medical Sciences
Volume: 4
Pages: 109-112
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Involvement of WNT Signaling in the Regulation of Gestational Age-Dependent Umbilical Cord-Derived Mesenchymal Stem Cell Proliferation.2017
Author(s)
7: Iwatani S, Shono A, Yoshida M, Yamana K, Thwin KKM, Kuroda J, Kurokawa D, Koda T, Nishida K, Ikuta T, Fujioka K, Mizobuchi M, Taniguchi-Ikeda M, Morioka I, Iijima K, Nishimura N.
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Journal Title
Stem Cells Int.
Volume: -
Pages: 1-16
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Gestational Age-Dependent Increase of Survival Motor Neuron Protein in Umbilical Cord-Derived Mesenchymal Stem Cells.2017
Author(s)
Iwatani S, Harahap NIF, Nurputra DK, Tairaku S, Shono A, Kurokawa D, Yamana K, Thwin KKM, Yoshida M, Mizobuchi M, Koda T, Fujioka K, Taniguchi-Ikeda M, Yamada H, Morioka I, Iijima K, Nishio H, Nishimura N.
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Journal Title
Front Pediatr.
Volume: -
Pages: 194-194
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Evaluation of BiliCare transcutaneous bilirubin device in Japanese newborns.2017
Author(s)
Yamana K, Morioka I, Kurokawa D, Fukushima S, Nishida K, Ohyama S, Nishimura N, Nozu K, Taniguchi-Ikeda M, Nagase H, Fujioka K, Iwatani S, Nakamura H, Iijima K.
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Journal Title
Pediatr Int.
Volume: 59(10)
Issue: 10
Pages: 1058-1063
DOI
Related Report
Peer Reviewed
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[Presentation] BRAIN MAGNETIC RESONANCE IMAGING FINDINGS IN IN INFANTS WITH CONGENITAL CYTOMEGALOVIRUS INFECTION EARLY IN LIFE2017
Author(s)
Nishida K, Iwatani S, Kurokawa D, Yamana K, Ohyama S, Fujioka K, Nagase H, Sugioka Y, Nishii T, Iijima K, Morioka I
Organizer
Pediatric Academic Societies Meeting
Related Report
Int'l Joint Research
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[Presentation] POSTNATAL AGE-SPECIFIC REFERENCE RANGES OF SERUM PROCALCITONIN LEVELS IN JAPANESE NEWBORN INFANTS2017
Author(s)
Morioka I, Iwatani S, Ohyama S, Yamana K, Nishida K, Kurokawa D, Fujioka K, Fukuzumi N, Sato I, Osawa K, Iijima K
Organizer
Pediatric Academic Societies Meeting
Related Report
Int'l Joint Research
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[Presentation] A new evaluation index for left-right unequal ventriculomegaly in very low birth weight infants: Association with walking disabilities at 1.6 years of corrected age2017
Author(s)
Ikuta T, Mizobuchi M, Katayama Y, Yoshimoto S, Ioroi T, Yamane M, Morisawa T, Takatera A, Ueda M, Shibata A, Maeyama K, Fujioka K, Nishimura N, Iijima K, Morioka I.
Organizer
The 2nd Congress of joint European Neonatal Society
Related Report
Int'l Joint Research
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