Establishment of the model of intestinal and gastric type gastric cancer using CRISPR/Cas9 and organoid
Project/Area Number |
16H06999
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Research Category |
Grant-in-Aid for Research Activity Start-up
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Allocation Type | Single-year Grants |
Research Field |
Experimental pathology
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Research Institution | Hiroshima University |
Principal Investigator |
Sakamoto Naoya 広島大学, 医歯薬保健学研究科(医), 助教 (20571798)
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Project Period (FY) |
2016-08-26 – 2018-03-31
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Project Status |
Completed (Fiscal Year 2017)
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Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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Keywords | オルガノイド / CRISPR/Cas9 / ゲノム編集 / 胃がん / 個別化医療 / 癌 / 胃癌 / ゲノム |
Outline of Final Research Achievements |
We have established the organoids from intestinal metaplasia and fundic/pyloric gland by using the selection of culture condition and FACS, and then try to introduce several genomic abnormalities that are characteristic especially in gastric cancer (GC) using CRISPR/Cas9 system. We also generated the organoids from GC cases with intestinal and gastric mucin phenotype, respectively. It was found that GC organoids with intestinal mucin phenotype showed more significant resistance against 5-FU than GC organoids with gastric mucin phenotype.
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Report
(3 results)
Research Products
(18 results)
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[Journal Article] Overexpression of PCDHB9 promotes peritoneal metastasis and correlates with poor prognosis in patients with gastric cancer.2017
Author(s)
Mukai S, Oue N, Oshima T, Imai T, Sekino Y, Honma R, Sakamoto N, Sentani K, Kuniyasu H, Egi H, Tanabe K, Yoshida K, Ohdan H, Yasui W.
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Journal Title
J Pathol
Volume: 243
Issue: 1
Pages: 100-110
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] BRAFV600E cooperates with CDX2 inactivation to promote serrated colorectal tumorigenesis.2017
Author(s)
Sakamoto N, Feng Y, Stolfi C, Kurosu Y, Green M, Lin J, Green ME, Sentani K, Yasui W, McMahon M, Hardiman KM, Spence JR, Horita N, Greenson JK, Kuick R, Cho KR, Fearon ER.
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Journal Title
Elife
Volume: 6
Pages: 6-6
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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