| Project/Area Number |
16H07001
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Neurology
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| Research Institution | Hiroshima University |
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Principal Investigator |
Ito Hikaru 広島大学, 医歯薬保健学研究科(医), 助教 (50587392)
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| Research Collaborator |
Asakura Atsushi University of Minnesota, Department of Neurology, Stem Cell Institute, Associate Professor
Asakura Yoko University of Minnesota, Department of Neurology, Stem Cell Institute, Technical staff(Researcher2)
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 再生医療 / 骨格筋幹細胞 / 胚盤胞胚補完法 / 発生工学 / 筋ジストロフィー / サルコペニア / 骨格筋 / キメラ / 再生医学 / 老化 / 移植・再生医療 / 発生・分化 / 動物 / 細胞・組織 |
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| Outline of Final Research Achievements |
One attractive therapy is myogenic stem cell transplantation to provide dystrophin in Duchenne Muscular Dystrophy (DMD) patients. Recent work has reported the induced pluripotent stem cell (iPSC)-derived myogenic stem cell transplantation for therapeutic strategy for DMD. However, there is a limitation in this strategy, especially in low differentiation efficiency and poor reproducibility of iPSCs-derived myogenic stem cells. Therefore, in a blastocyst complementation method, the regeneration of an organ can be achieved by utilizing the defect in an organ which can be complemented by injecting patient-derived iPSCs into a non-human blastocyst.
In this study, we injected GFP (+) mouse iPSCs into skeletal muscle-less mouse blastocysts. iPSC-derived cells highly contributed to limb muscle of chimera. We also performed intramuscular injection of myogenic stem cells isolated from chimeric mice into mdx mice, and detected GFP (+) muscle fibers which also expressed donor-derived dystrophin.
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