Epigenetic dynamics during mouse oogenesis

• Project/Area Number: 16H07044
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Genome biology
• Research Institution: Kyushu University
• Principal Investigator: Ishiuchi Takashi 九州大学, 生体防御医学研究所, 助教 (80612100)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: クロマチン / エピジェネティクス / 卵子形成 / エピゲノム

Outline of Final Research Achievements

In this study, we aimed to reveal the chromatin status during oogenesis. As it was reported previously that ATAC-seq method is useful to profile open chromatin regions with limited cell number, we focused on this technique. We tried to optimize the method. We examined the suitable method to dissociate Tn5 transposase during sample preparation. We believe that such optimization will be important to obtain stable results from limited cell number samples.