Development of animal models for anti-neurofascin 155 antibody-positive inflammatory demyelinating diseases by single chain antibodies

• Project/Area Number: 16H07051
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Neurology
• Research Institution: Kyushu University
• Principal Investigator: Hidenori Ogata 九州大学, 大学病院, 助教 (90778838)
• Research Collaborator: Ito Yuji 鹿児島大学, 理工学研究科, 教授 (60223195)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 慢性炎症性脱髄性多発根ニューロパチー / neurofascin 155 / 自己抗体 / フラグメント抗体 / ランビエ絞輪 / neurofascin155 / ファージディスプレイ / 慢性炎症性多発根ニューロパチー

Outline of Final Research Achievements

It has been reported that a subset of chronic inflammatory demyelinating polyneuropathy (CIDP) has autoantibodies against neurofascin 155 (NF155). However, pathogenicity of the antibodies remains to be elucidated. In this study, we aimed to isolate pathogenic single chain variable fragments against NF155 to elucidate pathomechanisms of anti-NF155 antibody-positive CIDP. We constructed an immunoglobulin V-gene phage-display library from the immunoglobulin repertoire of a patient with anti-NF155 antibody-positive CIDP. Then several antigen-specific clones were isolated using a cell panning method.

Research Products

• [Journal Article] Anti-neurofascin 155 antibody-related neuropathy (2018)
  • Author(s): Ogata Hidenori、Yamasaki Ryo
  • Journal Title: Clinical and Experimental Neuroimmunology Volume: 9 Issue: 1 Pages: 54-64
  • DOI: 10.1111/cen3.12444
  • Peer Reviewed
• [Journal Article] Parallel fluctuation of anti-neurofascin 155 antibody levels with clinico-electrophysiological findings in patients with chronic inflammatory demyelinating polyradiculoneuropathy (2018)
  • Author(s): Fujita Atsushi、Ogata Hidenori、Yamasaki Ryo、Matsushita Takuya、Kira Jun-ichi
  • Journal Title: Journal of the Neurological Sciences Volume: 384 Pages: 107-112
  • DOI: 10.1016/j.jns.2017.11.035
  • Peer Reviewed
• [Journal Article] ELectron microscopic abnormality and therapeutic efficacy in chronic inflammatory demyelinating polyneuropathy with anti-neurofascin155 immunoglobulin G4 antibody (2017)
  • Author(s): Kuwahara Motoi、Suzuki Hidekazu、Oka Nobuyuki、Ogata Hidenori、Yanagimoto Satoshi、Sadakane Shuji、Fukumoto Yuta、Yamana Masaki、Yuhara Yoshiko、Yoshikawa Keisuke、Morikawa Miyuki、Kawai Shigeru、Okazaki Masahiro、Tsujimoto Toru、Kira Jun-Ichi、Kusunoki Susumu
  • Journal Title: Muscle Nerve Volume: 57 Issue: 3 Pages: 498-502
  • DOI: 10.1002/mus.25757
  • Peer Reviewed
• [Journal Article] Paranodal dissection in chronic inflammatory demyelinating polyneuropathy with anti-neurofascin-155 and anti-contactin-1 antibodies. (2017)
  • Author(s): Koike H, Kadoya M, Kaida KI, Ikeda S, Kawagashira Y, Iijima M, Kato D, Ogata H, Yamasaki R, Matsukawa N, Kira JI, Katsuno M, Sobue G.
  • Journal Title: J Neurol Neurosurg Psychiatry. Volume: epub ahead of print Issue: 6 Pages: 465-473
  • DOI: 10.1136/jnnp-2016-314895
  • Peer Reviewed
• [Presentation] Proposal of diagnostic criteria for anti-neurofascin 155 antibody-associated neuropathy (2017)
  • Author(s): Ogata H, Yamasaki R, Matsushita T, Kadoya M, Kaida K, Matsui M, Kuwabara S, Kusunoki S, Kira JI.
  • Organizer: XXIII World Congress of Neurology
  • Int'l Joint Research
• [Presentation] 自己抗体陽性CIDPの臨床像 (2017)
  • Author(s): 緒方 英紀，山﨑 亮，吉良 潤一
  • Organizer: 第28回日本末梢神経学会学術集会
• [Presentation] Clinico-electrophysiological correlation with anti-neurofascin155 antibody levels in the antibody-positive chronic inflammatory demyelinating polyneuropathy (2017)
  • Author(s): Ogata H, Fujita A, Yamasaki R, Matsushita T, Kira JI.
  • Organizer: 2017 PNS Annual Meeting
  • Int'l Joint Research
• [Presentation] 抗neurofascin155抗体関連ニューロパチー暫定診断基準案の策定. (2017)
  • Author(s): 79)緒方英紀，山﨑亮，松下拓也，角谷真人，海田賢一，松井真，桑原聡，楠進，吉良潤一
  • Organizer: 第29回日本神経免疫学会学術集会.