Elucidation of COMMD1 effect in HIV-1 latent infection establishment

• Project/Area Number: 16H07080
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Virology
• Research Institution: Kumamoto University
• Principal Investigator: Kudo Eriko 熊本大学, エイズ学研究センター, 特定事業研究員 (00779176)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: HIV-1 / HIV-1宿主因子 / 潜伏感染 / COMMD1 / 転写制御 / ウイルス

Outline of Final Research Achievements

The effect of HIV-1 host factors has identified in primary infection. However, the effect in latent infection establishment is unknown. COMMD1 inducible cell line suppressed HIV-1 replication and decreased integrated HIV-1 genome. HIV-1 latently infection establishment and reactivation effect were similar between COMMD1 high and low expressed cells. Moreover, -1kbp COMMD1 promoter construct has highest activity in both myeloid and lymphoid cells. Therefore, this study indicates that COMMD1 doesn’t have effect for HIV-1 latent infection establishment and transcriptional regulation in myeloid cells was regulated in a further upstream.

Research Products

• [Journal Article] Restoring PU.1 induces apoptosis and modulates viral transactivation via interferon-stimulated genes in primary effusion lymphoma (2017)
  • Author(s): Goto H、Kariya R、Kudo E、Okuno Y、Ueda K、Katano H、Okada S
  • Journal Title: Oncogene Volume: 36 Issue: 37 Pages: 5252-5262
  • DOI: 10.1038/onc.2017.138
  • Peer Reviewed
• [Journal Article] nhibition of autophagy by chloroquine induces apoptosis in primary effusion lymphoma in vitro and in vivo through induction of endoplasmic reticulum stress. (2016)
  • Author(s): Masud Alam M, Kariya R, Kawaguchi A, Matsuda K, Kudo E, Okada S.
  • Journal Title: Apoptosis. Volume: 10 Issue: 10 Pages: 1191-1201
  • DOI: 10.1007/s10495-016-1277-7
  • Peer Reviewed / Open Access
• [Journal Article] Inhibition of carbonic anhydrase potentiates bevacizumab treatment in cholangiocarcinoma. (2016)
  • Author(s): Vaeteewoottacharn K, Kariya R, Dana P, Fujikawa S, Matsuda K, Ohkuma K, Kudo E, Kraiklang R, Wongkham C, Wongkham S, Okada S.
  • Journal Title: Tumour Biol. Volume: 7 Issue: 7 Pages: 9023-9035
  • DOI: 10.1007/s13277-016-4785-8
  • Peer Reviewed
• [Presentation] Sp1によるHIV-1潜伏感染の維持因子COMMD1の転写制御 (2016)
  • Author(s): Eriko Kudo, Manabu Taura, Seiji Okada
  • Organizer: 第64回日本ウイルス学会学術集会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2016-10-23
• [Remarks] 岡田プロジェクト研究室ホームページ
  • URL: http://www.caids.kumamoto-u.ac.jp/data/okada/default.html