The administration of ghrelin improved hepatocellular injury following parenteral feeding in a rat model of short bowel syndrome

• Project/Area Number: 16H07090
• Research Category: Grant-in-Aid for Research Activity Start-up
• Allocation Type: Single-year Grants
• Research Field: Pediatric surgery
• Research Institution: Kagoshima University
• Principal Investigator: onishi shun 鹿児島大学, 附属病院, 医員 (10614638)
• Project Period (FY): 2016-08-26 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 腸管不全関連肝障害 / グレリン / 短腸症候群 / 経静脈栄養 / 腸管順応 / 消化管ホルモン / 腸肝不全関連肝障害

Outline of Final Research Achievements

Purpose: Long-term parenteral nutrition following massive bowel resection causes liver dysfunction, such as intestinal failure-associated liver disease (IFALD).The aim of this study was to evaluate the effects of the administration of ghrelin in the liver in a parenterally fed rat model of short bowel syndrome (SBS).Methods: Rats underwent jugular vein catheterization, and were divided into 3 groups: 90% small bowel resection (90% SBR) and TPN (SBS/TPN group), 90% SBR and TPN plus ghrelin (SBS/TPN/ghrelin group), sham operation with normal chow (sham group). Ghrelin was administered continuously. Result: The AST and LDH levels significantly increased and the accumulation of lipids in the liver was observed in the TPN/SBS group. The accumulation of lipids in the liver of the rats in the SBS/TPN group was attenuated by the administration of ghrelin. Conclusion: The administration of ghrelin has a therapeutic potential for IFALD.

Research Products

• [Journal Article] The bowel function and quality of life of Hirschsprung disease patients who have reached 18 years of age or older ? the long-term outcomes after undergoing the transabdominal soave procedure (2017)
  • Author(s): Onishi Shun、Nakame Kazuhiko、Kaji Tatsuru、Kawano Masato、Moriguchi Tomoe、Sugita Koshiro、Yano Keisuke、Nomura Mioko、Yamada Koji、Yamada Waka、Masuya Ryuta、Kawano Takafumi、Machigashira Seiro、Mukai Motoi、Ieiri Satoshi
  • Journal Title: Journal of Pediatric Surgery Volume: 52 Issue: 12 Pages: 2001-2005
  • DOI: 10.1016/j.jpedsurg.2017.08.036
  • Peer Reviewed / Open Access
• [Journal Article] Long-term outcome of bowel function for 110 consecutive cases of Hirschsprung's disease: Comparison of the abdominal approach with transanal approach over 30 years in a single institution : Is the transanal approach truly beneficial for bowel function? (2016)
  • Author(s): Onishi S, Nakame K, Yamada K, Yamada W, Kawano T, Mukai M, Kaji T, Ieiri S
  • Journal Title: J Pediatr Surg Volume: 51(12) Issue: 12 Pages: 2010-2014
  • DOI: 10.1016/j.jpedsurg.2016.09.029
  • Peer Reviewed / Open Access
• [Journal Article] The administration of ghrelin improved hepatocellular injury following parenteral feeding in a rat model of short bowel syndrome. (2016)
  • Author(s): Onishi S, Kaji T, Yamada Y, Moriguchi T, Sugita K, Yamada K, Kawano T, Nakame K, Mukai M, Yamada S, Yoshioka T, Tanimoto A, Ieiri S
  • Journal Title: Pediatr Surg Int Volume: 32(12) Issue: 12 Pages: 1165-1171
  • DOI: 10.1007/s00383-016-3975-1
  • Peer Reviewed / Open Access
• [Journal Article] Ghrelin Improves Intestinal Mucosal Atrophy During Parenteral Nutrition: An Experimental Study (2016)
  • Author(s): Yamada W, Kaji T, Onishi S, Nakame K, Yamada K, Kawano T, Mukai M, Souda M, Yoshioka T, Tanimoto A, Ieiri S
  • Journal Title: J Pediatr Surg Volume: 51(12) Issue: 12 Pages: 2039-2043
  • DOI: 10.1016/j.jpedsurg.2016.09.035
  • Peer Reviewed / Open Access
• [Journal Article] Efficacy of Ethanol locks to reduce the incidence of catheter-related bloodstream infections for home parenteral nutrition pediatric patients. Comparison of therapeutic treatment with prophylactic treatment- (2016)
  • Author(s): Kawano T, Kaji T, Ohnishi S, Yamada K, Yamada W, Nakame K, Mukai M, Ieiri S
  • Journal Title: Pediatric Surgery International Volume: 32(9) Issue: 9 Pages: 863-867
  • DOI: 10.1007/s00383-016-3933-y
  • Peer Reviewed / Open Access
• [Presentation] 短腸症候群に対するグレリンを用いた残存腸管 順応と肝障害の克服-モデルラットによる実験的研究.Ghrelin improves Intestinal Mucosal Atrophy and Liver Damage in Short Bowel Syndrome Model Rats. (2017)
  • Author(s): 大西 峻,加治 建,森口智江,杉田光士郎,山田耕嗣,山田和歌,桝屋隆太,川野孝文,町頭成郎,中目和彦,向井 基,家入里志
  • Organizer: 第54回日本小児外科学会学術集会
• [Presentation] GHRELIN IMPROVES HEPATOCELLULAR INJURY FOLLOWING SHORT BOWEL SYNDROME IN A PARENTERAL NUTRITION RAT MODEL (2016)
  • Author(s): Shun Onishi
  • Organizer: 29th International Symposium on Pediatric Surgical Research
  • Place of Presentation: Frankfurt（Germany）
  • Year and Date: 2016-09-08
  • Int'l Joint Research