| Project/Area Number |
16H07121
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
Nakamura Tsukasa 京都府立医科大学, 医学(系)研究科(研究院), 助教 (30777959)
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 制御性T細胞 / 骨髄由来抑制細胞 / 臓器移植 / MDSCs / 免疫寛容 |
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| Outline of Final Research Achievements |
Background: Myeloid-derived suppressor cells (MDSCs) maintain host immunity: regulating rejection. On the other hand, CD8+ Tregs also play a key role in preventing rejection. However, the relationship between MDSCs and CD8+ Tregs is unclear. Here, the results revealed that MDSCs have a potential to recruit CD8+/IL-10+ Tregs. Methods: MDSCs were isolated from bone-marrow culture with GM-CSF, M-CSF, and dexamethasone (MDSCs-Dex). In murine heterotopic cardiac transplantation, MDSCs were transferred through the tail vein. Results: Flow cytometric analyses showed that MDSCs-Dex led to significant recruitment of CD8+/IL-10+ Tregs (3.3±2.1% vs 10.2±3.6%, p<0.05). In a MDSCs transfer model, pathological findings also confirmed accumulation of CD8+/PD-1+ Tregs in an allograft. Conclusion:MDSCs might result in recruitment of CD8+/IL-10+ Tregs. These results suggested that the synergistic effect between MDSCs and CD8+ Tregs developed a tendency to immunological tolerance.
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