| Project/Area Number |
16H07177
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Kidney internal medicine
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| Research Institution | Keio University |
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Principal Investigator |
Hiratsuka Ken 慶應義塾大学, 医学部(信濃町), 助教 (80594481)
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| Research Collaborator |
KO Minoru 慶應義塾大学, 医学部, 教授 (50631199)
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | ヒトES細胞 / 転写調節因子 / リプログラミング / 腎臓再生 / 多能制幹細胞 / 転写因子 / 再生医療 / 腎尿細管細胞 / ヒト多能性幹細胞 / 肝細胞 / 尿細管細胞 |
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| Outline of Final Research Achievements |
We utilized the transcription factor (TF)-inducible human ES lines, analyzed correlation of gene expression response to the induction of human TFs with tissue-specific gene expression in silico, and identified candidate TFs for differentiating towards renal lineages. Based on in-silico analysis, we have identified a set of four TFs that induce nephron progenitor cells (NPCs) and another set of four TFs that help differentiate toward pretubular aggregate-like cells. Two days after the transfection of the first set of four TFs together into hESCs, NPCs were induced efficiently (~92%). Subsequent administration of the second set of four TFs transfection induced differentiation into pretubular aggregate-like cells. On day 14, epithelial characteristic changes and multi-segmented kidney structures containing podocyte, proximal tubules, and distal tubules were observed in 3D cultures. RNA-Seq analysis also showed the global expression profile closely resembled human kidney profile.
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