Establishment of multi-dimensional analysis for monitoring pathophysiological status of pregnancy-induced hypertension
| Project/Area Number |
16H07187
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 妊娠高血圧症候群 / 疾患バイオマーカー / 多元的病態評価 / ターゲットプロテオミクス / peptidomics / LC-MS/MS / serum biomarkers / preeclampsia / pregnancy |
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| Outline of Final Research Achievements |
We previously developed potential serum disease biomarkers (DBMs) of hypertensive disorder of pregnancy (HDP). Our methodology did not extend to PDA071 (cysteinyl α2-HS-glycoprotein341-367), due to difficulty to produce a specific antibody against the peptide. The aim of the present study was to establish an alternative PDA071 quantitation system using LC-MRM/MS, to explore the potential utility of PDA071 as a DBM for HDP. PDA071 was successfully extracted from serum using a heat denaturation method. Optimum conditions for quantitation via LC-MRM/MS were developed. Although the PDA071 alone did not significantly differ between patients and controls, 3D plotting of PDA039/044/071 peptide levels and construction of a Jackknife classification matrix were satisfactory in terms of HDP diagnostic precision. Combination analysis using both PDA071 and PDA039/044 levels allowed HDP diagnostic accuracy to be attained, and our method will be valuable in future pathophysiological studies of HDP.
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Quantitative measurement of a candidate serum biomarker peptide derived from α2-HS-glycoprotein, and a preliminary trial of multidimensional peptide analysis in females with pregnancy-induced hypertension.2018
Author(s)
Hamamura K, Yanagida M, Ishikawa H, Banzai M, Yoshitake H, Nonaka D, Tanaka K, Sakuraba M, Miyakuni Y, Takamori K, Nojima M, Yoshida K, Fujiwara H, Takeda S, Araki Y.
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Journal Title
Ann Clin Biochem
Volume: 55
Issue: 2
Pages: 287-295
DOI
Related Report
Peer Reviewed
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