| Project/Area Number |
16H07187
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| Research Category |
Grant-in-Aid for Research Activity Start-up
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| Allocation Type | Single-year Grants |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2016-08-26 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 妊娠高血圧症候群 / 疾患バイオマーカー / 多元的病態評価 / ターゲットプロテオミクス / peptidomics / LC-MS/MS / serum biomarkers / preeclampsia / pregnancy |
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| Outline of Final Research Achievements |
We previously developed potential serum disease biomarkers (DBMs) of hypertensive disorder of pregnancy (HDP). Our methodology did not extend to PDA071 (cysteinyl α2-HS-glycoprotein341-367), due to difficulty to produce a specific antibody against the peptide. The aim of the present study was to establish an alternative PDA071 quantitation system using LC-MRM/MS, to explore the potential utility of PDA071 as a DBM for HDP. PDA071 was successfully extracted from serum using a heat denaturation method. Optimum conditions for quantitation via LC-MRM/MS were developed. Although the PDA071 alone did not significantly differ between patients and controls, 3D plotting of PDA039/044/071 peptide levels and construction of a Jackknife classification matrix were satisfactory in terms of HDP diagnostic precision. Combination analysis using both PDA071 and PDA039/044 levels allowed HDP diagnostic accuracy to be attained, and our method will be valuable in future pathophysiological studies of HDP.
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