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Elucidation of the role of NADPH oxidase in cartilage degenerative diseases

Research Project

Project/Area Number 16H07195
Research Category

Grant-in-Aid for Research Activity Start-up

Allocation TypeSingle-year Grants
Research Field Prosthodontics/ Dental materials science and
Research InstitutionShowa University

Principal Investigator

INOUE SAKIE  昭和大学, 歯学部, 助教 (20783252)

Project Period (FY) 2016-08-26 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords軟骨 / 軟骨変性疾患 / NADPHオキシダーゼ / 軟骨細胞 / 細胞外マトリックス / NADPH-オキシダーゼ
Outline of Final Research Achievements

In this study, we found that NADPH oxidase (NOX)-2 activity-dependent acidification induced activation of hyaluronidase in chondrocyte cultures, which caused reduction in extracellular matrix. In rheumatoid arthritis, bone resorption progresses in addition to cartilage degradation. Hence, we studied the molecular mechanism of bone resorption occurs in an NO- and ROS-dependent manner. We found that 8-nitro-cGMP, a novel downstream signaling molecule of NO and ROS, was produced by osteoclasts and its progenitor cells in an NADPH oxidase-dependent manner after stimulation by inflammatory cytokines. We also found that 8-nitro-cGMP promoted osteoclast differentiation.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Annual Research Report
  • Research Products

    (7 results)

All 2018 2017 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 2 results,  Acknowledgement Compliant: 1 results) Presentation (3 results) Remarks (2 results)

  • [Journal Article] 8-Nitro-cGMP is a promoter of osteoclast differentiation induced by RANKL2018

    • Author(s)
      Kaneko K.、Miyamoto Y.、Tsukuura R.、Sasa K.、Akaike T.、Fujii S.、Yoshimura K.、Nagayama K.、Hoshino M.、Inoue S.、Maki K.、Baba K.、Chikazu D.、Kamijo R.
    • Journal Title

      Nitric Oxide

      Volume: 72 Pages: 46-51

    • DOI

      10.1016/j.niox.2017.11.006

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Extracellular matrix loss in chondrocytes after exposure to interleukin-1β in NADPH oxidase-dependent manner.2017

    • Author(s)
      Funato S, Yasuhara R, Yoshimura K, Miyamoto Y, Kaneko K, Suzawa T, Chikazu D, Mishima K, Baba K, Kamijo R
    • Journal Title

      Cell Tissue Res

      Volume: 368 Issue: 1 Pages: 135-144

    • DOI

      10.1007/s00441-016-2551-2

    • Related Report
      2016 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Presentation] インターロイキン-1β刺激後の軟骨細胞様ATDC5細胞におけるNADPHオキシダーゼに依存した細胞外基質の減少2017

    • Author(s)
      船登咲映、安原理佳、宮本洋一、吉村健太郎、美島健二、馬場一美、上條竜太郎
    • Organizer
      第71回日本口腔科学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] NADPHオキシダーゼ依存的軟骨マトリックスの喪失2017

    • Author(s)
      笹 清人、吉村 健太郎、宮本 洋一、船登 咲映、安原理佳、美島健二、馬場一美、上條竜太郎
    • Organizer
      第70回日本酸化ストレス学会学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] NADPHオキシダーゼ-2に依存した軟骨基質の減少2017

    • Author(s)
      宮本洋一、船登咲映、安原理佳、吉村健太郎、金子児太郎、近津大地、美島健二、馬場一美、上條竜太郎
    • Organizer
      第3回日本骨免疫学会
    • Related Report
      2017 Annual Research Report
  • [Remarks] 昭和大学歯学部口腔生化学講座

    • URL

      http://www10.showa-u.ac.jp/~oralbio/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 昭和大学歯学部歯科補綴学講座

    • URL

      http://prostho.showa-u.ac.jp/

    • Related Report
      2017 Annual Research Report

URL: 

Published: 2016-09-02   Modified: 2019-03-29  

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